E. coli is indeed the coolest thing on the planet - Carl Zimmer
hsdS:Quickview
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| References | Suggestions |
| Gene Name | |
|---|---|
| Product(s) |
HsdS product[1]: specificity determinant for hsdM and hsdR product, B4348 product, Hss product, Hsp product |
| Description |
specificity determinant for hsdM and hsdR[2][3] Component of EcoKI restriction-modification system[3] |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| edit table | |
See Help:Quickview for help with entering information in the Quickview table.
Notes
The restriction-modification complex EcoKI is comprised of three polypeptide subunits coded for by the genes hsdS, hsdM, and hsdR. HsdS is the polypeptide that directly interacts with DNA through the use of two target recognition domain (TRD) sequences first isolated by Fuller-Pace and Murray.[4] HsdS recognizes and binds to the nucleotide sequence 5' AAC(N)6GTGC. Fuller-Pace and Murray determined this sequence of the TRDs in hsdS after recombining two homologous hsdS genes from a family of restriction-modification complexes.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ Fuller-Pace FV & Murray NE (1986) Two DNA recognition domains of the specificity polypeptides of a family of type I restriction enzymes. Proc Natl Acad Sci U S A 83: 9368-72 PubMed EcoliWiki page
