EcoliHub logo

Try the new EcoliHub and let us know what you'd like to see.

hslV:On One Page

From EcoliWiki

Jump to: navigation, search
Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]



See Help:Quickview for help with entering information in the Quickview table.

Standard Name

hslV

Gene Synonym(s)

ECK3924, b3932, JW3903, htpO, clpQ, yiiC[1]

Product Desc.

peptidase component of the HslVU protease[2][3];

Component of HslV hexamer[3]; HslVU protease[3]

Heat-inducible ATP-dependent protease HslVU, protease subunit; involved in the degradation of misfolded proteins[4]

Product Synonyms(s)

peptidase component of the HslUV protease[1], B3932[2][1], ClpQ[2][1], YiiC[2][1], HtpO[2][1], HslV[2][1]

Function from GO

Cellular Component

Biological Process

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hslVU[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(hslV)    Textpresso(hslV)

edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]





Gene

[back to top]


Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hslV

Mnemonic

heat shock locus Heat-shock locus

Synonyms

ECK3924, b3932, JW3903, htpO, clpQ, yiiC[1]

edit table

Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

88.79 minutes 

MG1655: 4120310..4119780
gbrowse_img.php?coord=4119780..4120310&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4101770..4101240
gbrowse_img.php?coord=4101240..4101770&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3514394..3514924
gbrowse_img.php?coord=3514394..3514924&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4220007..4219477
gbrowse_img.php?coord=4219477..4220007&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 4009449..4009979
gbrowse_img.php?coord=4009449..4009979&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

edit table

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
edit table

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔhslV (Keio:JW3903)

deletion

deletion

PMID:16738554

Shigen
CGSC10818[5]

ΔhslV720::kan

PMID:16738554

CGSC:101424


edit table

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW3903

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACAACTATAGTAAGCGTACG

Primer 2:CCCGCTTTGTAGCTTAATTCTTC

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[5]

est. P1 cotransduction: 7% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[5]

est. P1 cotransduction: 42% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11676

EchoBASE (EcoliWiki Page)

EchoBASE:EB1627

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3932

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4885

EcoGene (EcoliWiki Page)

EcoGene:EG11676

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HSLV

CGSC (EcoliWiki Page)

CGSC:34160

ASAP (EcoliWikiPage)

ASAP:ABE-0012845


edit table

Notes

Links

[back to top]



Product(s)

[back to top]



Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HslV

Synonyms

peptidase component of the HslUV protease[1], B3932[2][1], ClpQ[2][1], YiiC[2][1], HtpO[2][1], HslV[2][1]

Product description

peptidase component of the HslVU protease[2][3];

Component of HslV hexamer[3]; HslVU protease[3]

Heat-inducible ATP-dependent protease HslVU, protease subunit; involved in the degradation of misfolded proteins[4]

EC number (for enzymes)
edit table

Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0004298

threonine-type endopeptidase activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001353
SP_KW:KW-0888

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0005839

proteasome core complex

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001353

C

Seeded from EcoCyc [7]

complete

GO:0006511

ubiquitin-dependent protein catabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001353

P

Seeded from EcoCyc [7]

complete

GO:0006950

response to stress

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc [7]

complete

GO:0008233

peptidase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0645

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [7]

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0006508

proteolysis

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006457

protein folding

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009050

glycopeptide catabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0000910

cytokinesis

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of HslV hexamer

could be indirect

Protein

deaD

PMID:15690043

Experiment(s):EBI-880750

Protein

hslU

PMID:15690043

Experiment(s):EBI-880750, EBI-886932

Protein

rplB

PMID:15690043

Experiment(s):EBI-880750

Protein

tpiA

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplC

PMID:15690043

Experiment(s):EBI-880750, EBI-886859

Protein

yggN

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplD

PMID:15690043

Experiment(s):EBI-880750, EBI-886859

Protein

yghT

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplI

PMID:15690043

Experiment(s):EBI-880750

Protein

prs

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplJ

PMID:15690043

Experiment(s):EBI-880750

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplS

PMID:15690043

Experiment(s):EBI-880750

Protein

rplV

PMID:15690043

Experiment(s):EBI-880750, EBI-886859

Protein

rpsG

PMID:15690043

Experiment(s):EBI-880750

Protein

rpsM

PMID:15690043

Experiment(s):EBI-880750

Protein

tufA

PMID:15690043

Experiment(s):EBI-880750

Protein

rluB

PMID:15690043

Experiment(s):EBI-880750

Protein

accA

PMID:15690043

Experiment(s):EBI-886859

Protein

accB

PMID:15690043

Experiment(s):EBI-886859

Protein

accC

PMID:15690043

Experiment(s):EBI-886859

Protein

accD

PMID:15690043

Experiment(s):EBI-886859

Protein

ccmB

PMID:15690043

Experiment(s):EBI-886859

Protein

gabP

PMID:15690043

Experiment(s):EBI-886859

Protein

rplL

PMID:15690043

Experiment(s):EBI-886859

Protein

rplU

PMID:15690043

Experiment(s):EBI-886859

Protein

rplX

PMID:15690043

Experiment(s):EBI-886859

Protein

rpmC

PMID:15690043

Experiment(s):EBI-886859

Protein

rpmG

PMID:15690043

Experiment(s):EBI-886859

Protein

rpsP

PMID:15690043

Experiment(s):EBI-886859

Protein

yihI

PMID:15690043

Experiment(s):EBI-886859

Protein

yhjD

PMID:15690043

Experiment(s):EBI-886859

Protein

Subunits of HslVU protease

could be indirect


edit table

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
edit table

Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MTTIVSVRRN GHVVIAGDGQ ATLGNTVMKG NVKKVRRLYN DKVIAGFAGG TADAFTLFEL
FERKLEMHQG HLVKAAVELA KDWRTDRMLR KLEALLAVAD ETASLIITGN GDVVQPENDL
IAIGSGGPYA QAAARALLEN TELSAREIAE KALDIAGDIC IYTNHFHTIE ELSYKA
Length

176

Mol. Wt

19.092 kDa

pI

6.4 (calculated)

Extinction coefficient

11,460 - 11,585 (calc based on 4 Y, 1 W, and 1 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed (Probable)

UniProt:P0A7B8


edit table


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11676-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0A7B8

PFAM (EcoliWiki Page)

PFAM:PF00227

RefSeq (EcoliWiki Page)

RefSeq:NP_418367


edit table

Notes

Links

[back to top]




Expression

[back to top]



Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hslVU

edit table
Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


gbrowse_img.php?coord=4120290..4120330&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b3932 (EcoliWiki Page)

NCBI GEO profiles for hslV

microarray

GenExpDB:b3932 (EcoliWiki Page)

Summary of data for hslV from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (4119788..4120017) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ04; Well:E2[9]

edit table

Notes

Accessions Related to hslV Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11676

EchoBASE (EcoliWiki Page)

EchoBASE:EB1627

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3932

EcoGene (EcoliWiki Page)

EcoGene:EG11676


edit table

Notes

Links

[back to top]



Evolution

[back to top]



Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Dictyostelium discoideum

  • DDB0190148 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

HSLV

From SHIGELLACYC

E. coli O157

HSLV

From ECOO157CYC

edit table


Do-It-Yourself Web Tools

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00227


edit table

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

[back to top]


Retrieved from "http://ecoliwiki.net/colipedia/index.php/hslV:On_One_Page"
Personal tools