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hyfI:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hyfI

Mnemonic

Hydrogenase

Synonyms

ECK2485, b2489, JW5805[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

56.23 minutes 

MG1655: 2608728..2609486
gbrowse_img.php?coord=2608728..2609486&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2531526..2532284
gbrowse_img.php?coord=2531526..2532284&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2609362..2610120
gbrowse_img.php?coord=2609362..2610120&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2700493..2701251
gbrowse_img.php?coord=2700493..2701251&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2494533..2495291
gbrowse_img.php?coord=2494533..2495291&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔhyfI (Keio:JW5805)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11588[3]

ΔhyfI737::kan

PMID:16738554[2]

CGSC:101494


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW5805

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGTCCAGTGCTTACACAACA

Primer 2:CCGTCATGTAACCCCCGGAGTAC

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[3]

est. P1 cotransduction: 59% [5]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[3]

est. P1 cotransduction: 7% [5]
Synonyms:zff-208::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7306

EchoBASE (EcoliWiki Page)

EchoBASE:EB3969

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2489

EcoGene (EcoliWiki Page)

EcoGene:EG14217

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HYFI

ASAP (EcoliWikiPage)

ASAP:ABE-0008198


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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