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hyfI:On One Page

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Standard Name

hyfI

Gene Synonym(s)

ECK2485, b2489, JW5805[1]

Product Desc.

hydrogenase 4, small subunit[2][3];

Component of hydrogenase 4[3]

Putative hydrogenase 4 component I; membrane protein of Hyf electron transfer complex of FHL system[4]

Product Synonyms(s)

hydrogenase 4, Fe-S subunit[1], B2489[2][1], HyfI[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hyfABCDEFGHIJR-focB[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(hyfI)    Textpresso(hyfI)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hyfI

Mnemonic

Hydrogenase

Synonyms

ECK2485, b2489, JW5805[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

56.23 minutes 

MG1655: 2608728..2609486
gbrowse_img.php?coord=2608728..2609486&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2531526..2532284
gbrowse_img.php?coord=2531526..2532284&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2609362..2610120
gbrowse_img.php?coord=2609362..2610120&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2700493..2701251
gbrowse_img.php?coord=2700493..2701251&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2494533..2495291
gbrowse_img.php?coord=2494533..2495291&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔhyfI (Keio:JW5805)

deletion

deletion

PMID:16738554

Shigen
CGSC11588[5]

ΔhyfI737::kan

PMID:16738554

CGSC:101494


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW5805

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTCCAGTGCTTACACAACA

Primer 2:CCGTCATGTAACCCCCGGAGTAC

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[5]

est. P1 cotransduction: 59% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[5]

est. P1 cotransduction: 7% [6]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7306

EchoBASE (EcoliWiki Page)

EchoBASE:EB3969

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2489

EcoGene (EcoliWiki Page)

EcoGene:EG14217

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HYFI

ASAP (EcoliWikiPage)

ASAP:ABE-0008198


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HyfI

Synonyms

hydrogenase 4, Fe-S subunit[1], B2489[2][1], HyfI[2][1]

Product description

hydrogenase 4, small subunit[2][3];

Component of hydrogenase 4[3]

Putative hydrogenase 4 component I; membrane protein of Hyf electron transfer complex of FHL system[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0005506

iron ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc [7]

complete

GO:0006120

mitochondrial electron transport, NADH to ubiquinone

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006137
InterPro:IPR006138

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0008137

NADH dehydrogenase (ubiquinone) activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006137
InterPro:IPR006138

F

Seeded from EcoCyc [7]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc [7]

complete

GO:0016651

oxidoreductase activity, acting on NADH or NADPH

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014406

F

Seeded from EcoCyc [7]

complete

GO:0046872

metal ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc [7]

complete

GO:0051536

iron-sulfur cluster binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0411

F

Seeded from EcoCyc [7]

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR014406
SP_KW:KW-0004

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0055114

oxidation reduction

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc [7]

complete

GO:0009061

anaerobic respiration

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of hydrogenase 4

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MSPVLTQHVS QPITLDEQTQ KMKRHLLQDI RRSAYVYRVD CGGCNACEIE IFAAITPVFD
AERFGIKVVS SPRHADILLF TGAVTRAMRM PALRAYESAP DHKICVSYGA CGVGGGIFHD
LYSVWGGSDT IVPIDVWIPG CPPTPAATIH GFAVALGLLQ QKIHAVDYRD PTGVTMQPLW
PQIPPSQRIA IEREARRLAG YRQGREICDR LLRHLSDDPT GNRVNTWLRD ADDPRLNSIV
QQLFRVLRGL HD
Length

252

Mol. Wt

28.1 kDa

pI

8.1 (calculated)

Extinction coefficient

32,430 - 33,305 (calc based on 7 Y, 4 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:MONOMER0-151

UniProt (EcoliWiki Page)

UniProt:P77668

PFAM (EcoliWiki Page)

PFAM:PF01058

RefSeq (EcoliWiki Page)

RefSeq:NP_416984


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hyfABCDEFGHIJR-focB

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2608708..2608748&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2489 (EcoliWiki Page)

NCBI GEO profiles for hyfI

microarray

GenExpDB:b2489 (EcoliWiki Page)

Summary of data for hyfI from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to hyfI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7306

EchoBASE (EcoliWiki Page)

EchoBASE:EB3969

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2489

EcoGene (EcoliWiki Page)

EcoGene:EG14217


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

HYFI

From SHIGELLACYC

E. coli O157

HYFI

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01058


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

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