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ileS:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

IleS

Synonyms

isoleucyl-tRNA synthetase[1], B0026[2][1], IlvS[2][1], IleS[2][1]

Product description

ILES[2][3]

Isoleucine--tRNA ligase[4]

EC number (for enzymes)

Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0005515

protein binding

PMID:15690043[5]

IPI: Inferred from Physical Interaction

UniProtKB:P0A6N1

F

Seeded from EcoCyc [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002301
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0547

F

Seeded from EcoCyc [6]

complete

GO:0004812

aminoacyl-tRNA ligase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0030

F

Seeded from EcoCyc [6]

complete

GO:0004822

isoleucine-tRNA ligase activity

GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002301
EC:6.1.1.5
HAMAP:MF_02002

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002301
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0067
HAMAP:MF_02002

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005737

cytoplasm

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002301
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [7]

complete

GO:0006412

translation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002301
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0648

P

Seeded from EcoCyc [6]

complete

GO:0006418

tRNA aminoacylation for protein translation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155

P

Seeded from EcoCyc [6]

complete

GO:0006428

isoleucyl-tRNA aminoacylation

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002301
HAMAP:MF_02002

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0008270

zinc ion binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR010663
SP_KW:KW-0862

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0016874

ligase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc [6]

complete

GO:0046677

response to antibiotic

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc [6]

complete

GO:0046872

metal ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc [6]

complete

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010663

F

Seeded from EcoCyc [6]

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

yccE

PMID:16606699[8]

Experiment(s):EBI-1135335

Protein

dnaJ

PMID:16606699[8]

Experiment(s):EBI-1135335

Protein

glpD

PMID:16606699[8]

Experiment(s):EBI-1135335

Protein

tufA

PMID:15690043[5]

Experiment(s):EBI-889355

Protein

hyaD

PMID:15690043[5]

Experiment(s):EBI-886272

Protein

ppk

PMID:15690043[5]

Experiment(s):EBI-886272

Protein

rbsB

PMID:15690043[5]

Experiment(s):EBI-886272

Protein

allR

PMID:15690043[5]

Experiment(s):EBI-886272

Protein

yjgL

PMID:15690043[5]

Experiment(s):EBI-886272

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc

MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT FILHDGPPYA
NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL PIELKVEQEY GKPGEKFTAA
EFRAKCREYA ATQVDGQRKD FIRLGVLGDW SHPYLTMDFK TEANIIRALG KIIGNGHLHK
GAKPVHWCVD CRSALAEAEV EYYDKTSPSI DVAFQAVDQD ALKAKFAVSN VNGPISLVIW
TTTPWTLPAN RAISIAPDFD YALVQIDGQA VILAKDLVES VMQRIGVTDY TILGTVKGAE
LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDYVIGQKYG LETANPVGPD
GTYLPGTYPT LDGVNVFKAN DIVVALLQEK GALLHVEKMQ HSYPCCWRHK TPIIFRATPQ
WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA RIESMVANRP DWCISRQRTW GVPMSLFVHK
DTEELHPRTL ELMEEVAKRV EVDGIQAWWD LDAKEILGDE ADQYVKVPDT LDVWFDSGST
HSSVVDVRPE FAGHAADMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG
RKMSKSIGNT VSPQDVMNKL GADILRLWVA STDYTGEMAV SDEILKRAAD SYRRIRNTAR
FLLANLNGFD PAKDMVKPEE MVVLDRWAVG CAKAAQEDIL KAYEAYDFHE VVQRLMRFCS
VEMGSFYLDI IKDRQYTAKA DSVARRSCQT ALYHIAEALV RWMAPILSFT ADEVWGYLPG
EREKYVFTGE WYEGLFGLAD SEAMNDAFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV
TLYAEPELSA KLTALGDELR FVLLTSGATV ADYNDAPADA QQSEVLKGLK VALSKAEGEK
CPRCWHYTQD VGKVAEHAEI CGRCVSNVAG DGEKRKFA
Length

938

Mol. Wt

104.297 kDa

pI

5.8 (calculated)

Extinction coefficient

175,670 - 177,420 (calc based on 33 Y, 23 W, and 14 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P00956



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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:ILES-MONOMER

UniProt (EcoliWiki Page)

UniProt:P00956

PFAM (EcoliWiki Page)

PFAM:PF00133

RefSeq (EcoliWiki Page)

RefSeq:NP_414567


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 5.5 5.6 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. 7.0 7.1 7.2 7.3 7.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. 8.0 8.1 8.2 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page

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