EcoliHub logo

Try the new EcoliHub and let us know what you'd like to see.

ilvC:On One Page

From EcoliWiki

Jump to: navigation, search
Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]



See Help:Quickview for help with entering information in the Quickview table.

Standard Name

ilvC

Gene Synonym(s)

ECK3766, b3774, JW3747[1]

Product Desc.

Ketol-acid reductoisomerase[2]

Product Synonyms(s)

ketol-acid reductoisomerase, NAD(P)-binding[1], B3774[3][1], IlvC[3][1]

Function from GO

Molecular Function

Cellular Component

Biological Process

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ilvC[3], OP00127

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(ilvC)    Textpresso(ilvC)

edit table

See Help:Quickview for help with entering information in the Quickview table.

Notes

[back to top]





Gene

[back to top]


Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ilvC

Mnemonic

Isoleucine-valine (requirement)

Synonyms

ECK3766, b3774, JW3747[1]

edit table

Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

85.26 minutes 

MG1655: 3955993..3957468
gbrowse_img.php?coord=3955993..3957468&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3919772..3921247
gbrowse_img.php?coord=3919772..3921247&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3678711..3677236
gbrowse_img.php?coord=3677236..3678711&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4054913..4056388
gbrowse_img.php?coord=4054913..4056388&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 3845662..3847137
gbrowse_img.php?coord=3845662..3847137&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

edit table

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
edit table

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔilvC (Keio:JW3747)

deletion

deletion

PMID:16738554

Shigen
CGSC10735[4]

ilvC::Tn5KAN

PMID:12519969

ASAP

At position 992 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB21448

ilvC7

CGSC:5079

ilvC49

CGSC:9330

ilvC44

CGSC:13346

ΔilvC725::kan

PMID:16738554

CGSC:101546


edit table

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW3747

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTAACTACTTCAATACACT

Primer 2:CCACCCGCAACAGCAATACGTTT

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[4]

est. P1 cotransduction: 91% [5]
Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[4]

est. P1 cotransduction: 8% [5]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10495

EchoBASE (EcoliWiki Page)

EchoBASE:EB0490

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3774

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4696

EcoGene (EcoliWiki Page)

EcoGene:EG10495

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ILVC

CGSC (EcoliWiki Page)

CGSC:607

ASAP (EcoliWikiPage)

ASAP:ABE-0012331


edit table

Notes

Links

[back to top]



Product(s)

[back to top]



Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

IlvC

Synonyms

ketol-acid reductoisomerase, NAD(P)-binding[1], B3774[3][1], IlvC[3][1]

Product description

Ketol-acid reductoisomerase[2]

EC number (for enzymes)
edit table

Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0004455

ketol-acid reductoisomerase activity

PMID:2653423

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005515

protein binding

PMID:16606699

IPI: Inferred from Physical Interaction

UniProtKB:P16918

F

Seeded from EcoCyc [7]

complete

GO:0005829

cytosol

PMID:2653423

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [7]

complete

GO:0009097

isoleucine biosynthetic process

PMID:4562389

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0009099

valine biosynthetic process

PMID:2653423

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0004455

ketol-acid reductoisomerase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR000506
InterPro:IPR014359
EC:1.1.1.86
HAMAP:MF_00435

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0009097

isoleucine biosynthetic process

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00435

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0009099

valine biosynthetic process

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00435

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0050661

NADP or NADPH binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014359

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[8].

required fields missing

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [7]

complete

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [7]

complete

GO:0008152

metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc [7]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR013116
SP_KW:KW-0028

P

Seeded from EcoCyc [7]

complete

GO:0009082

branched chain family amino acid biosynthetic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000506
InterPro:IPR013023
InterPro:IPR014359
SP_KW:KW-0100

P

Seeded from EcoCyc [7]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR008927
InterPro:IPR013023
SP_KW:KW-0560

F

Seeded from EcoCyc [7]

complete

GO:0055114

oxidation reduction

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc [7]

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

rhsC

PMID:16606699

Experiment(s):EBI-1146665

edit table

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

cytoplasm


edit table

Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MANYFNTLNL RQQLAQLGKC RFMGRDEFAD GASYLQGKKV VIVGCGAQGL NQGLNMRDSG
LDISYALRKE AIAEKRASWR KATENGFKVG TYEELIPQAD LVINLTPDKQ HSDVVRTVQP
LMKDGAALGY SHGFNIVEVG EQIRKDITVV MVAPKCPGTE VREEYKRGFG VPTLIAVHPE
NDPKGEGMAI AKAWAAATGG HRAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSLLCFDKL
VEEGTDPAYA EKLIQFGWET ITEALKQGGI TLMMDRLSNP AKLRAYALSE QLKEIMAPLF
QKHMDDIISG EFSSGMMADW ANDDKKLLTW REETGKTAFE TAPQYEGKIG EQEYFDKGVL
MIAMVKAGVE LAFETMVDSG IIEESAYYES LHELPLIANT IARKRLYEMN VVISDTAEYG
NYLFSYACVP LLKPFMAELQ PGDLGKAIPE GAVDNGQLRD VNEAIRSHAI EQVGKKLRGY
MTDMKRIAVA G
Length

491

Mol. Wt

54.069 kDa

pI

5.1 (calculated)

Extinction coefficient

52,830 - 53,580 (calc based on 17 Y, 5 W, and 6 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P05793


edit table


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

edit table

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:KETOLREDUCTOISOM-MONOMER

UniProt (EcoliWiki Page)

UniProt:P05793

PFAM (EcoliWiki Page)

PFAM:PF01450

RefSeq (EcoliWiki Page)

RefSeq:NP_418222


edit table

Notes

Links

[back to top]




Expression

[back to top]



Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ilvC

edit table
Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


gbrowse_img.php?coord=3955973..3956013&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b3774 (EcoliWiki Page)

NCBI GEO profiles for ilvC

microarray

GenExpDB:b3774 (EcoliWiki Page)

Summary of data for ilvC from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (3955350..3955645) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ01; Well:C2[9]

edit table

Notes

Accessions Related to ilvC Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10495

EchoBASE (EcoliWiki Page)

EchoBASE:EB0490

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3774

EcoGene (EcoliWiki Page)

EcoGene:EG10495


edit table

Notes

Links

[back to top]



Evolution

[back to top]



Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000001059 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT3G58610 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q65XK0 (score: 1.000; bootstrap: 100%)
  • Q5VPB9 (score: 0.849)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YLR355C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC56F22 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000025332 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ILVC

From SHIGELLACYC

E. coli O157

ILVC

From ECOO157CYC

edit table


Do-It-Yourself Web Tools

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01450


edit table

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

[back to top]


Retrieved from "http://ecoliwiki.net/colipedia/index.php/ilvC:On_One_Page"
Personal tools