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kefC:On One Page

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Standard Name

kefC

Gene Synonym(s)

ECK0048, b0047, JW0046, trkC[1]

Product Desc.

KefC potassium CPA2 transporter[2][3]

NEM-activatable K+/H+ antiporter[4]

Product Synonyms(s)

potassium:proton antiporter[1], B0047[2][1], TrkC[2][1], KefC[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): kefF[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(kefC)    Textpresso(kefC)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

kefC

Mnemonic

K+ efflux

Synonyms

ECK0048, b0047, JW0046, trkC[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.03 minutes 

MG1655: 47769..49631
gbrowse_img.php?coord=47769..49631&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 51964..53826
gbrowse_img.php?coord=51964..53826&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 47769..49631
gbrowse_img.php?coord=47769..49631&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 47769..49631
gbrowse_img.php?coord=47769..49631&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 47769..49631
gbrowse_img.php?coord=47769..49631&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔkefC (Keio:JW0046)

deletion

deletion

PMID:16738554

Shigen
CGSC8359[5]

kefC121

CGSC:8149

ΔkefC760::kan

PMID:16738554

CGSC:101626


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0046

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGATAGCCATACGCTGATTCA

Primer 2:CCGGATGAGGGTTTCGTTTCCGG

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 58% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 23% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10521

EchoBASE (EcoliWiki Page)

EchoBASE:EB0516

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0047

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0057

EcoGene (EcoliWiki Page)

EcoGene:EG10521

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:KEFC

CGSC (EcoliWiki Page)

CGSC:82

ASAP (EcoliWikiPage)

ASAP:ABE-0000161


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

KefC

Synonyms

potassium:proton antiporter[1], B0047[2][1], TrkC[2][1], KefC[2][1]

Product description

KefC potassium CPA2 transporter[2][3]

NEM-activatable K+/H+ antiporter[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [7]

complete

GO:0006811

ion transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0406

P

Seeded from EcoCyc [7]

complete

GO:0006812

cation transport

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004771

P

Seeded from EcoCyc [7]

complete

GO:0006813

potassium ion transport

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR003148
InterPro:IPR006036
SP_KW:KW-0633
HAMAP:MF_01413

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0006885

regulation of pH

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006153

P

Seeded from EcoCyc [7]

complete

GO:0008324

cation transmembrane transporter activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004771
InterPro:IPR006036

F

Seeded from EcoCyc [7]

complete

GO:0015297

antiporter activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0050

F

Seeded from EcoCyc [7]

complete

GO:0015299

solute:hydrogen antiporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006153

F

Seeded from EcoCyc [7]

complete

GO:0015503

glutathione-regulated potassium exporter activity

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01413

F

Seeded from EcoCyc [7]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004771
InterPro:IPR006153
SP_KW:KW-0812

C

Seeded from EcoCyc [7]

complete

GO:0030955

potassium ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0630

F

Seeded from EcoCyc [7]

complete

GO:0015292

uniporter activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [7]

complete

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [7]

complete

GO:0005886

plasma membrane

GO_REF:0000020
GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

HAMAP:MF_01413
SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [7]

complete

GO:0008152

metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc [7]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

plasma membrane

C-terminus localized in the cytoplasm with 13 predicted transmembrane domains

Daley et al. (2005) [9]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MDSHTLIQAL IYLGSAALIV PIAVRLGLGS VLGYLIAGCI IGPWGLRLVT DAESILHFAE
IGVVLMLFII GLELDPQRLW KLRAAVFGCG ALQMVICGGL LGLFCMLLGL RWQVAELIGM
TLALSSTAIA MQAMNERNLM VTQMGRSAFA VLLFQDIAAI PLVAMIPLLA TSSASTTMGA
FALSALKVAG ALVLVVLLGR YVTRPALRFV ARSGLREVFS AVALFLVFGF GLLLEEVGLS
MAMGAFLAGV LLASSEYRHA LESDIEPFKG LLLGLFFIGV GMSIDFGTLL ENPLRIVILL
LGFLIIKIAM LWLIARPLQV PNKQRRWFAV LLGQGSEFAF VVFGAAQMAN VLEPEWAKSL
TLAVALSMAA TPILLVILNR LEQSSTEEAR EADEIDEEQP RVIIAGFGRF GQITGRLLLS
SGVKMVVLDH DPDHIETLRK FGMKVFYGDA TRMDLLESAG AAKAEVLINA IDDPQTNLQL
TEMVKEHFPH LQIIARARDV DHYIRLRQAG VEKPERETFE GALKTGRLAL ESLGLGPYEA
RERADVFRRF NIQMVEEMAM VENDTKARAA VYKRTSAMLS EIITEDREHL SLIQRHGWQG
TEEGKHTGNM ADEPETKPSS
Length

620

Mol. Wt

67.798 kDa

pI

6.0 (calculated)

Extinction coefficient

50,420 - 50,920 (calc based on 8 Y, 7 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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tmhmm.php?gene=kefC&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:KEFC-MONOMER

UniProt (EcoliWiki Page)

UniProt:P03819

PFAM (EcoliWiki Page)

PFAM:PF02254

RefSeq (EcoliWiki Page)

RefSeq:NP_414589


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

kefF

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=47749..47789&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0047 (EcoliWiki Page)

NCBI GEO profiles for kefC

microarray

GenExpDB:b0047 (EcoliWiki Page)

Summary of data for kefC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to kefC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10521

EchoBASE (EcoliWiki Page)

EchoBASE:EB0516

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0047

EcoGene (EcoliWiki Page)

EcoGene:EG10521


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Arabidopsis thaliana

  • AT1G01790 (score: 1.000; bootstrap: 99%)
  • AT4G00630 (score: 0.833)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000020850 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00009617 (score: 1.000; bootstrap: 100%)
  • WBGene00010214 (score: 0.678)
  • WBGene00009618 (score: 0.274)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000009546 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005318 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0219541 (score: 1.000; bootstrap: 100%)
  • DDB0184575 (score: 0.148)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000027112 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000279934 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000004640 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000016169 (score: 0.217)

From Inparanoid:20070104

Mus musculus

  • MGI:2444946 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000010303 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000026281 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YJL094C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC1051c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00017938001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000038550 (score: 1.000; bootstrap: 74%)

From Inparanoid:20070104

Shigella flexneri

KEFC

From SHIGELLACYC

E. coli O157

KEFC

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF02254


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ 8.0 8.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Daley DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308: 1321-3 PubMed EcoliWiki page

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