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Standard Name

kefF

Gene Synonym(s)

ECK0047, b0046, JW0045, yabF[1]

Product Desc.

regulator of KefC-mediated potassium transport[2][3]

Required for KefC-mediated K+ efflux; member of quinone oxidoreductase family, but lacks key residues needed for activity[4]

Product Synonyms(s)

flavoprotein subunit for the KefC potassium efflux system[1], B0046[2][1], KefF[2][1], YabF[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): kefF[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(kefF)    Textpresso(kefF)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

kefF

Mnemonic

K+ efflux

Synonyms

ECK0047, b0046, JW0045, yabF[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

1.02 minutes 

MG1655: 47246..47776
gbrowse_img.php?coord=47246..47776&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 51441..51971
gbrowse_img.php?coord=51441..51971&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 47246..47776
gbrowse_img.php?coord=47246..47776&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 47246..47776
gbrowse_img.php?coord=47246..47776&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 47246..47776
gbrowse_img.php?coord=47246..47776&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔkefF (Keio:JW0045)

deletion

deletion

PMID:16738554

Shigen
CGSC8358[5]

ΔkefF759::kan

PMID:16738554

CGSC:101628


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0045

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATTCTTATAATTTATGCGCA

Primer 2:CCTCCATGATGGGCCTCCTGCCA

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 59% [6]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 23% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11568

EchoBASE (EcoliWiki Page)

EchoBASE:EB1528

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0046

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0056

EcoGene (EcoliWiki Page)

EcoGene:EG11568

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YABF

ASAP (EcoliWikiPage)

ASAP:ABE-0000159


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

KefF

Synonyms

flavoprotein subunit for the KefC potassium efflux system[1], B0046[2][1], KefF[2][1], YabF[2][1]

Product description

regulator of KefC-mediated potassium transport[2][3]

Required for KefC-mediated K+ efflux; member of quinone oxidoreductase family, but lacks key residues needed for activity[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0006813

potassium ion transport

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01414

P

Seeded from EcoCyc [7]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003680

F

Seeded from EcoCyc [7]

complete

GO:0050662

coenzyme binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003680

F

Seeded from EcoCyc [7]

complete

GO:0006810

transport

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009055

electron carrier activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003680

F

Seeded from EcoCyc [7]

complete

GO:0008152

metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

fhlA

PMID:16606699

Experiment(s):EBI-1135438

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1135438

Protein

thrS

PMID:16606699

Experiment(s):EBI-1135438

Protein

groL

PMID:16606699

Experiment(s):EBI-1135438

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MILIIYAHPY PHHSHANKRM LEQARTLEGV EIRSLYQLYP DFNIDIAAEQ EALSRADLIV
WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL LWAVTTGGGE SHFEIGAHPG
FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI CDDETLEGQA RHYKQRLLEW QEAHHG
Length

176

Mol. Wt

20.169 kDa

pI

6.3 (calculated)

Extinction coefficient

50,420 - 50,795 (calc based on 8 Y, 7 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11568-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0A754

PFAM (EcoliWiki Page)

PFAM:PF02525

RefSeq (EcoliWiki Page)

RefSeq:NP_414588


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

kefF

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=47226..47266&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0046 (EcoliWiki Page)

NCBI GEO profiles for kefF

microarray

GenExpDB:b0046 (EcoliWiki Page)

Summary of data for kefF from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (47050..47270) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ18; Well:A3[8]

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Notes

Accessions Related to kefF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11568

EchoBASE (EcoliWiki Page)

EchoBASE:EB1528

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0046

EcoGene (EcoliWiki Page)

EcoGene:EG11568


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000000402 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000034446 (score: 1.000; bootstrap: 100%)
  • ENSAPMP00000022018 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000039940 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000032251 (score: 0.410)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000013713 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000030044 (score: 0.362)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030131-1226 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040425-1145 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000020892 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000337773 (score: 1.000; bootstrap: 100%)
  • ENSP00000319788 (score: 0.359)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000011126 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000025865 (score: 0.360)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000003283 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Mus musculus

  • MGI:103187 (score: 1.000; bootstrap: 100%)
  • MGI:104513 (score: 0.320)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000030178 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000014148 (score: 0.361)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000017175 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000024141 (score: 0.338)
  • ENSRNOP00000033201 (score: 0.303)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000145774 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

YABF

From SHIGELLACYC

E. coli O157

YABF

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF02525


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

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