Standard name	LeuS
Synonyms	leucyl-tRNA synthetase^[1], B0642^[2]^[1], Syl^[2]^[1], LeuS^[2]^[1], LeuRS^[2]^[1]
Product description	LEUS^[2]^[3]
EC number (for enzymes)	6.1.1.4^[1]
edit table

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Notes

Function

[back to top]

Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004823	leucine-tRNA ligase activity	PMID:10024478^[4]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0006429	leucyl-tRNA aminoacylation	PMID:10024478^[4]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0000166	nucleotide binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001412 InterPro:IPR002300 InterPro:IPR002302 InterPro:IPR009008 InterPro:IPR009080 InterPro:IPR013155 SP_KW:KW-0547	F	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0004823	leucine-tRNA ligase activity	GO_REF:0000002 GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002302 EC:6.1.1.4 HAMAP:MF_00049	F	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0005524	ATP binding	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR001412 InterPro:IPR002300 InterPro:IPR002302 InterPro:IPR009008 InterPro:IPR009080 InterPro:IPR013155 SP_KW:KW-0067 HAMAP:MF_00049	F	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0005737	cytoplasm	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001412 InterPro:IPR002300 InterPro:IPR002302 InterPro:IPR009008 InterPro:IPR009080 InterPro:IPR013155 SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0006412	translation	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001412 InterPro:IPR002300 InterPro:IPR002302 InterPro:IPR009008 InterPro:IPR009080 InterPro:IPR013155 SP_KW:KW-0648	P	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0006429	leucyl-tRNA aminoacylation	GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002302 HAMAP:MF_00049	P	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0006418	tRNA aminoacylation for protein translation				P	Seeded from EcoCyc 10.6^[2].	required fields missing
	GO:0004823	leucine-tRNA ligase activity	PMID:10024478^[4]	IDA		F	Seeded from EcoCyc 11.1^[3].	required fields missing
edit table

Interactions

Partner Type	Partner	Notes	References
edit table

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Notes

Localization

Compartment	Description	Evidence	Notes
edit table

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Notes

Structure and Physical Properties

[back to top]

Physical Properties

Sequence	at EcoCyc MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD NTLTVYTTRP DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS KYGLNIKPVI LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA DKLTAMGVGE RKVNYRLRDW GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG MPALRETDTF DTFMESSWYY ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY FRFFHKLMRD AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PADMTLEWQE SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA LRRDVHKTIA KVTDDIGRRQ TFNTAIAAIM ELMNKLAKAP TDGEQDRALM QEALLAVVRM LNPFTPHICF TLWQELKGEG DIDNAPWPVA DEKAMVEDST LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD GVTVRKVIYV PGKLLNLVVG
Mol. Wt	97.23 kDa (calc) ^[2]
pI
Extinction coefficient	169140 - 170515 (calc based on 36 Y, 21 W, and 11 C residues)
edit table

See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Domains/Motifs/Modification Sites

Type	Residues	Description	Notes
edit table

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Structure

Structures	Representative Escherichia coli structure: 2AJG\|A 11-196 of 860 residues (E-value: 2.1e-98) (Percent Identity: 100.00) Representative homolog: 1H3N\|A (Thermus thermophilus) 273-812 of 860 residues (E-value: 1.1e-99) (Percent Identity: 41.73) View all other structures.
Models	View models at: EcoliPredict ModBase PSI-PRED Protein Quaternary Structure (PQS) Genomes TO Protein (GTOP)
edit table

Structures

Representative Escherichia coli structure:
2AJG|A
11-196 of 860 residues (E-value: 2.1e-98) (Percent Identity: 100.00)
Representative homolog:
1H3N|A (Thermus thermophilus)
273-812 of 860 residues (E-value: 1.1e-99) (Percent Identity: 41.73)
View all other structures.

Models

View models at:

edit table

See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Notes

Gene Product Resources

[back to top]

Resource type	Source	Notes/Reference
edit table

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Notes

Accessions in Other Databases

[back to top]

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:LEUS-MONOMER
UniProt (EcoliWiki Page)	UniProt:P07813
PFAM (EcoliWiki Page)	PFAM:PF00133
RefSeq (EcoliWiki Page)	RefSeq:NP_415175
ModBase (EcoliWiki Page)	ModBase:P07813
PDB (EcoliWiki Page)	PDB:2AJI
edit table

See Help:Product_accessions for help entering or editing information in this section of EcoliWiki.

Notes

Links

[back to top]

Name	URL	Comments
edit table

See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 Chen J et al. (1999) High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli. Protein Expr Purif 15: 115-20 PubMed EcoliWiki page
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 ^5.6 ^5.7 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

leuS:Gene Product(s)

From EcoliWiki

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

Views

Personal tools

Navigation

Search

Toolbox