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leuS:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LeuS

Synonyms

leucyl-tRNA synthetase[1], B0642[2][1], Syl[2][1], LeuS[2][1], LeuRS[2][1]

Product description

LEUS[2][3]

Leucine--tRNA ligase[4]

EC number (for enzymes)

Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0004823

leucine-tRNA ligase activity

PMID:10024478[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0006429

leucyl-tRNA aminoacylation

PMID:10024478[5]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002302
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0547

F

Seeded from EcoCyc [7]

complete

GO:0004823

leucine-tRNA ligase activity

GO_REF:0000002
GO_REF:0000003
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002302
EC:6.1.1.4
HAMAP:MF_00049

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002302
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0067
HAMAP:MF_00049

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005737

cytoplasm

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002302
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0006412

translation

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR002302
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0648

P

Seeded from EcoCyc [7]

complete

GO:0006429

leucyl-tRNA aminoacylation

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002302
HAMAP:MF_00049

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0004812

aminoacyl-tRNA ligase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001412
InterPro:IPR002300
InterPro:IPR009008
InterPro:IPR009080
InterPro:IPR013155
SP_KW:KW-0030

F

Seeded from EcoCyc [7]

complete

GO:0016874

ligase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc [7]

complete

GO:0004823

leucine-tRNA ligase activity

PMID:10024478[5]

IDA

F

Seeded from EcoCyc 11.1[3].

required fields missing

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

htrE

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

map

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rplC

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rplD

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rplK

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rplL

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rpsB

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rpsJ

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rpsP

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

yeaP

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

rplW

PMID:15690043[8]

Experiment(s):EBI-891845

Protein

yjbH

PMID:16606699[9]

Experiment(s):EBI-1137534

Protein

hycE

PMID:16606699[9]

Experiment(s):EBI-1137534

Protein

groL

PMID:16606699[9]

Experiment(s):EBI-1137534

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc

MQEQYRPEEI ESKVQLHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD
VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKMLGFGYD
WSRELATCTP EYYRWEQKFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD
TKVERKEIPQ WFIKITAYAD ELLNDLDKLD HWPDTVKTMQ RNWIGRSEGV EITFNVNDYD
NTLTVYTTRP DTFMGCTYLA VAAGHPLAQK AAENNPELAA FIDECRNTKV AEAEMATMEK
KGVDTGFKAV HPLTGEEIPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAS KYGLNIKPVI
LAADGSEPDL SQQALTEKGV LFNSGEFNGL DHEAAFNAIA DKLTAMGVGE RKVNYRLRDW
GVSRQRYWGA PIPMVTLEDG TVMPTPDDQL PVILPEDVVM DGITSPIKAD PEWAKTTVNG
MPALRETDTF DTFMESSWYY ARYTCPQYKE GMLDSEAANY WLPVDIYIGG IEHAIMHLLY
FRFFHKLMRD AGMVNSDEPA KQLLCQGMVL ADAFYYVGEN GERNWVSPVD AIVERDEKGR
IVKAKDAAGH ELVYTGMSKM SKSKNNGIDP QVMVERYGAD TVRLFMMFAS PADMTLEWQE
SGVEGANRFL KRVWKLVYEH TAKGDVAALN VDALTENQKA LRRDVHKTIA KVTDDIGRRQ
TFNTAIAAIM ELMNKLAKAP TDGEQDRALM QEALLAVVRM LNPFTPHICF TLWQELKGEG
DIDNAPWPVA DEKAMVEDST LVVVQVNGKV RAKITVPVDA TEEQVRERAG QEHLVAKYLD
GVTVRKVIYV PGKLLNLVVG
Length

860

Mol. Wt

97.233 kDa

pI

5.1 (calculated)

Extinction coefficient

169,140 - 170,515 (calc based on 36 Y, 21 W, and 11 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:LEUS-MONOMER

UniProt (EcoliWiki Page)

UniProt:P07813

PFAM (EcoliWiki Page)

PFAM:PF00133

RefSeq (EcoliWiki Page)

RefSeq:NP_415175


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 Chen J et al. (1999) High-level expression and single-step purification of leucyl-tRNA synthetase from Escherichia coli. Protein Expr Purif 15: 115-20 PubMed EcoliWiki page
  6. 6.0 6.1 6.2 6.3 6.4 6.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. 7.0 7.1 7.2 7.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
  9. 9.0 9.1 9.2 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page

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