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Quickview

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See Help:Quickview for help with entering information in the Quickview table.

Standard Name

lon

Gene Synonym(s)

ECK0433, b0439, JW0429, capR, deg, dir, muc, lopA[1]

Product Desc.

Lon[2][3];

Component of DNA-binding, ATP-dependent protease La[3]

DNA-binding, ATP-dependent protease LA; lon mutants form long cells[4]

Product Synonyms(s)

DNA-binding ATP-dependent protease La[1], B0439[2][1], Muc[2][1], Dir[2][1], Deg[2][1], LopA[2][1], CapR[2][1], Lon[2][1]

Function from GO

Molecular Function

Biological Process

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): lon[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(lon)    Textpresso(lon)

See Help:Quickview for help with entering information in the Quickview table.

Notes

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Lon dependent lethality is due to induction of yefM-yoeb toxin/antitoxin pair.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lon

Mnemonic

Long form

Synonyms

ECK0433, b0439, JW0429, capR, deg, dir, muc, lopA[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

9.87 minutes 

MG1655: 458112..460466
gbrowse_img.php?coord=458112..460466&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 430943..433297
gbrowse_img.php?coord=430943..433297&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 458112..460466
gbrowse_img.php?coord=458112..460466&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 397443..399797
gbrowse_img.php?coord=397443..399797&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 360871..363225
gbrowse_img.php?coord=360871..363225&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Δlon (Keio:JW0429)

deletion

deletion

PMID:16738554

Shigen
CGSC8592[5]

lon

PMID:12519969

ASAP

At position 1865 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20175

lonK362A

K362A

Loss of proteolytic activity and ATP-binding

seeded from UniProt:P0A9M0

lonS679A

S679A

Loss of proteolytic activity

seeded from UniProt:P0A9M0

lon-11

CGSC:4788

lon-21

CGSC:5770

lon-22

CGSC:6957

lon-1::IS186

CGSC:7591

lon-20

CGSC:7652

lon-10

CGSC:7752

lon-6

CGSC:9311

lon-7

CGSC:9313

lon-9

CGSC:11065

lon-201

CGSC:19616

lon-202

CGSC:19617

Δlon-725::kan

PMID:16738554

CGSC:101680


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0429

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAATCCTGAGCGTTCTGAACG

Primer 2:CCTTTTGCAGTCACAACCTGCAT

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[5]

est. P1 cotransduction: 36% [6]

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[5]

est. P1 cotransduction: 96% [6]
Synonyms:zba-3054::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10542

EchoBASE (EcoliWiki Page)

EchoBASE:EB0537

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0439

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0555

EcoGene (EcoliWiki Page)

EcoGene:EG10542

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:LON

CGSC (EcoliWiki Page)

CGSC:547

ASAP (EcoliWikiPage)

ASAP:ABE-0001521


Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Lon

Synonyms

DNA-binding ATP-dependent protease La[1], B0439[2][1], Muc[2][1], Dir[2][1], Deg[2][1], LopA[2][1], CapR[2][1], Lon[2][1]

Product description

Lon[2][3];

Component of DNA-binding, ATP-dependent protease La[3]

DNA-binding, ATP-dependent protease LA; lon mutants form long cells[4]

EC number (for enzymes)

Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0017111

nucleoside-triphosphatase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc [7]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [7]

complete

GO:0008233

peptidase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0645

F

Seeded from EcoCyc [7]

complete

GO:0006950

response to stress

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc [7]

complete

GO:0004176

ATP-dependent peptidase activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003111
InterPro:IPR004815

F

Seeded from EcoCyc [7]

complete

GO:0006508

proteolysis

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984
InterPro:IPR008268
InterPro:IPR008269

P

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003959
InterPro:IPR004815
SP_KW:KW-0067

F

Seeded from EcoCyc [7]

complete

GO:0004252

serine-type endopeptidase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001984
InterPro:IPR008268
InterPro:IPR008269
SP_KW:KW-0720

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0004176

ATP-dependent peptidase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001984
InterPro:IPR003111
InterPro:IPR004815
InterPro:IPR008268
InterPro:IPR008269

F

Seeded from EcoCyc [7]

complete

GO:0003824

catalytic activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0021

F

Seeded from EcoCyc [7]

complete

GO:0003677

DNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc [7]

complete

GO:0000166

nucleotide binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003593
SP_KW:KW-0547

F

Seeded from EcoCyc [7]

complete

GO:0008236

serine-type peptidase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0720

F

Seeded from EcoCyc [7]

complete

GO:0009050

glycopeptide catabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0000910

cytokinesis

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0004176

ATP-dependent peptidase activity

PMID:6458036

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

PMID:15037242

IPI: Inferred from Physical Interaction

F

complete

GO:0016887

ATPase activity

PMID:6458036

IDA: Inferred from Direct Assay

F

complete

GO:0008233

peptidase activity

PMID:9720920

IDA: Inferred from Direct Assay

F

complete

GO:0006508

proteolysis

PMID:6458036

IDA: Inferred from Direct Assay

P

complete

GO:0004176

ATP-dependent peptidase activity

PMID:9720920

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

PMID:8995294

IDA: Inferred from Direct Assay

C

complete

GO:0003677

DNA binding

PMID:8995294

IDA: Inferred from Direct Assay

F

complete

GO:0006950

response to stress

PMID:6330035

IEP: Inferred from Expression Pattern

P

complete

GO:0004252

serine-type endopeptidase activity

PMID:15606774

IMP: Inferred from Mutant Phenotype

F

complete

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of DNA-binding, ATP-dependent protease La

could be indirect

Protein

ssb

PMID:15690043

Experiment(s):EBI-894571

Protein

rplC

PMID:15690043

Experiment(s):EBI-889577

Protein

rplI

PMID:15690043

Experiment(s):EBI-889577, EBI-894571

Protein

rplM

PMID:15690043

Experiment(s):EBI-889577, EBI-894571

Protein

rplT

PMID:15690043

Experiment(s):EBI-889577

Protein

rpsB

PMID:15690043

Experiment(s):EBI-889577

Protein

rpsC

PMID:15690043

Experiment(s):EBI-889577

Protein

tufA

PMID:15690043

Experiment(s):EBI-889577

Protein

fsaA

PMID:15690043

Experiment(s):EBI-894571

Protein

rplU

PMID:15690043

Experiment(s):EBI-894571

Protein

rplV

PMID:15690043

Experiment(s):EBI-894571

Protein

rpsG

PMID:15690043

Experiment(s):EBI-894571

Protein

rpsH

PMID:15690043

Experiment(s):EBI-894571

Protein

yjgL

PMID:15690043

Experiment(s):EBI-894571

Protein

dnaX

PMID:16606699

Experiment(s):EBI-1136659

Protein

sapF

PMID:16606699

Experiment(s):EBI-1136659

Protein

secB

PMID:16606699

Experiment(s):EBI-1136659

Protein

dnaA

PMID:16606699

Experiment(s):EBI-1136659

Protein

nadE

PMID:16606699

Experiment(s):EBI-1136659

Small Molecule

Diisopropyl fluorophosphate

PMID:6458037

Small Molecule

Mg2+

PMID:6458037

Data was not shown, but indicated that the presence of both Mg2+ and ATP were required for proteolytic activity.

Small Molecule

ATP

PMID:6458037

See evidence for Mg2+

Notes

For a review of Lon and other proteases, see Proteases and their targets in Escherichia coli. Gottesman (1996)[9]

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc

MNPERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSIRCL EAAMDHDKKI MLVAQKEAST
DEPGVNDLFT VGTVASILQM LKLPDGTVKV LVEGLQRARI SALSDNGEHF SAKAEYLESP
TIDEREQEVL VRTAISQFEG YIKLNKKIPP EVLTSLNSID DPARLADTIA AHMPLKLADK
QSVLEMSDVN ERLEYLMAMM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE
LGEMDDAPDE NEALKRKIDA AKMPKEAKEK AEAELQKLKM MSPMSAEATV VRGYIDWMVQ
VPWNARSKVK KDLRQAQEIL DTDHYGLERV KDRILEYLAV QSRVNKIKGP ILCLVGPPGV
GKTSLGQSIA KATGRKYVRM ALGGVRDEAE IRGHRRTYIG SMPGKLIQKM AKVGVKNPLF
LLDEIDKMSS DMRGDPASAL LEVLDPEQNV AFSDHYLEVD YDLSDVMFVA TSNSMNIPAP
LLDRMEVIRL SGYTEDEKLN IAKRHLLPKQ IERNALKKGE LTVDDSAIIG IIRYYTREAG
VRGLEREISK LCRKAVKQLL LDKSLKHIEI NGDNLHDYLG VQRFDYGRAD NENRVGQVTG
LAWTEVGGDL LTIETACVPG KGKLTYTGSL GEVMQESIQA ALTVVRARAE KLGINPDFYE
KRDIHVHVPE GATPKDGPSA GIAMCTALVS CLTGNPVRAD VAMTGEITLR GQVLPIGGLK
EKLLAAHRGG IKTVLIPFEN KRDLEEIPDN VIADLDIHPV KRIEEVLTLA LQNEPSGMQV VTAK
Length

784

Mol. Wt

87.44 kDa

pI

6.2 (calculated)

Extinction coefficient

46,300 - 47,050 (calc based on 20 Y, 3 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10542-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0A9M0

PFAM (EcoliWiki Page)

PFAM:PF02190

RefSeq (EcoliWiki Page)

RefSeq:NP_414973

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:LON-MONOMER


Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

lon

Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=458092..458132&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0439 (EcoliWiki Page)

NCBI GEO profiles for lon

microarray

GenExpDB:b0439 (EcoliWiki Page)

Summary of data for lon from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (457843..458176) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:G9[10]

Notes

Accessions Related to lon Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10542

EchoBASE (EcoliWiki Page)

EchoBASE:EB0537

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0439

EcoGene (EcoliWiki Page)

EcoGene:EG10542


Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000013687 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000030012 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G47040 (score: 1.000; bootstrap: 77%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000002350 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00033694 (score: 1.000; bootstrap: 86%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00016391 (score: 1.000; bootstrap: 90%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000014765 (score: 1.000; bootstrap: 54%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000018656 (score: 1.000; bootstrap: 83%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-041212-1 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-041212-2 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0204395 (score: 1.000; bootstrap: 100%)
  • DDB0217928 (score: 0.072)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036892 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21329-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000006273 (score: 1.000; bootstrap: 69%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000285737 (score: 1.000; bootstrap: 51%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000009707 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000001578 (score: 1.000; bootstrap: 57%)

From Inparanoid:20070104

Mus musculus

  • MGI:1914137 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Oryza gramene

  • P93647 (score: 1.000; bootstrap: 64%)
  • Q94F60 (score: 0.940)
  • Q8GV57 (score: 0.916)
  • Q69SH2 (score: 0.902)
  • Q6RS97 (score: 0.896)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000013818 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000020770 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YBL022C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC22F36c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000129607 (score: 1.000; bootstrap: 92%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00005077001 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000017719 (score: 1.000; bootstrap: 64%)

From Inparanoid:20070104

Shigella flexneri

LON

From SHIGELLACYC

E. coli O157

LON

From ECOO157CYC


Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF02190


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. 8.0 8.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. Gottesman S (1996) Proteases and their targets in Escherichia coli. Annu Rev Genet 30: 465-506 PubMed EcoliWiki page
  10. Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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