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lptA:On One Page

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See Help:Quickview for help with entering information in the Quickview table.

Standard Name

lptA

Gene Synonym(s)

yhbN, ECK3189, b3200, JW3167[1][2]

Product Desc.

LptA[2][3];

Component of YhbG/YhbN ABC transporter[2]; LptA/LptB ABC transporter[3]

Periplasmic LPS-binding protein, binds Lipid A domain; LPS transport and assembly[4]

Product Synonyms(s)

predicted transporter subunit: periplasmic-binding component of ABC superfamily[1], B3200[2][1], YhbN[2][1]

Function from GO

Biological Process

Molecular Function

Knock-Out Phenotype
Regulation/Expression

transcription unit(s):

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(lptA)    Textpresso(lptA)

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Notes

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LptA depletion results in rifampicin/bile salts sensitivity, indicative of an outer membrane integrity defect. Paralogous to Imp. The first 27 aa are a type I signal peptide. RpoE regulon.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lptA

Mnemonic

LPS transport

Synonyms

yhbN, ECK3189, b3200, JW3167[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

72.02 minutes 

MG1655: 3341402..3341959
gbrowse_img.php?coord=3341402..3341959&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3278615..3279172
gbrowse_img.php?coord=3278615..3279172&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3343235..3343792
gbrowse_img.php?coord=3343235..3343792&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 3228550..3229107
gbrowse_img.php?coord=3228550..3229107&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

lptAR76D,E

R76D,E

No change in activity

seeded from UniProt:P0ADV1

lptAF95A

F95A

No change in activity

seeded from UniProt:P0ADV1

lptAI38E

I38E

No change in activity

seeded from UniProt:P0ADV1

lptAI36D,E

I36D,E

No change in activity

seeded from UniProt:P0ADV1

lptAI38D

I38D

Decrease in activity

seeded from UniProt:P0ADV1

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7665

EchoBASE (EcoliWiki Page)

EchoBASE:EB2502

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3200

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3960

EcoGene (EcoliWiki Page)

EcoGene:EG12618

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YHBN

ASAP (EcoliWikiPage)

ASAP:ABE-0010510


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YhbN

Synonyms

predicted transporter subunit: periplasmic-binding component of ABC superfamily[1], B3200[2][1], YhbN[2][1]

Product description

LptA[2][3];

Component of YhbG/YhbN ABC transporter[2]; LptA/LptB ABC transporter[3]

Periplasmic LPS-binding protein, binds Lipid A domain; LPS transport and assembly[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0042597

periplasmic space

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc 12.5 [5]

complete

GO:0015921

lipopolysaccharide export

PMID:17056748

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [6]

complete

GO:0017089

glycolipid transporter activity

PMID:17056748

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc [6]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of YhbG/YhbN ABC transporter

could be indirect

Protein

yfcS

PMID:16606699

Experiment(s):EBI-1145137

Protein

ahpC

PMID:16606699

Experiment(s):EBI-1145137

Protein

sdhB

PMID:16606699

Experiment(s):EBI-1145137

Small Molecule

LPS

PMID:18480051


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Periplasm

PMID:9298646

EchoLocation:yhbN

periplasm

From EcoCyc[3]

Periplasm

PMID:18480051


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Notes

Localization was demonstrated by identification of overexpressed protein in the soluble fraction of E. coli lysates[7].

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MKFKTNKLSL NLVLASSLLA ASIPAFAVTG DTDQPIHIES DQQSLDMQGN VVTFTGNVIV
TQGTIKINAD KVVVTRPGGE QGKEVIDGYG KPATFYQMQD NGKPVEGHAS QMHYELAKDF
VVLTGNAYLQ QVDSNIKGDK ITYLVKEQKM QAFSDKGKRV TTVLVPSQLQ DKNNKGQTPA
QKKGN
Length

185

Mol. Wt

20.126 kDa

pI

9.4 (calculated)

Extinction coefficient

7,450 (calc based on 5 Y, W, and C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

motif

1-27

UniProt Manual:Signal Peptides

UniProt:P0ADV1


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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:YHBN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0ADV1

PFAM (EcoliWiki Page)

PFAM:PF03968

RefSeq (EcoliWiki Page)

RefSeq:NP_417667


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=3341382..3341422&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b3200 (EcoliWiki Page)

NCBI GEO profiles for lptA

microarray

GenExpDB:b3200 (EcoliWiki Page)

Summary of data for lptA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to lptA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:G7665

EchoBASE (EcoliWiki Page)

EchoBASE:EB2502

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3200

EcoGene (EcoliWiki Page)

EcoGene:EG12618


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

YHBN

From SHIGELLACYC

E. coli O157

YHBN

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF03968


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ 6.0 6.1 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ Tran AX et al. (2008) The LptA protein of Escherichia coli is a periplasmic lipid A binding protein involved in the lipopolysaccharide export pathway. J Biol Chem PubMed EcoliWiki page

Categories

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