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mcrA:On One Page

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Quickview | Gene | Product(s) | Expression| Evolution | References |

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See Help:Quickview for help with entering information in the Quickview table.

Standard Name

mcrA

Gene Synonym(s)

ECK1145, b1159, JW1145, rglA[1]

Product Desc.

restriction of DNA at 5-methylcytosine residues[2][3]

5-methylcytosine-specific restriction endonuclease B, e14; prophage gene[4]

Product Synonyms(s)

e14 prophage; 5-methylcytosine-specific restriction endonuclease B[1], B1159[2][1], RglA[2][1], McrA[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mcrA[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(mcrA)    Textpresso(mcrA)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mcrA

Mnemonic

Modified cytosine restriction

Synonyms

ECK1145, b1159, JW1145, rglA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

26.07 minutes 

MG1655: 1209569..1210402
gbrowse_img.php?coord=1209569..1210402&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1211923..1212756
gbrowse_img.php?coord=1211923..1212756&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔmcrA (Keio:JW1145)

deletion

deletion

PMID:16738554

Shigen
CGSC9065[5]

mcrA0

CGSC:10892

ΔmcrA747::kan

PMID:16738554

CGSC:101770


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1145

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCATGTTTTTGATAATAATGG

Primer 2:CCTTTCTGTAATCGGTTTATATT

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[5]

est. P1 cotransduction: 7% [6]

zcf-117::Tn10

Linked marker

CAG18463 = CGSC7363[5]

est. P1 cotransduction: 96% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10573

EchoBASE (EcoliWiki Page)

EchoBASE:EB0568

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1159

EcoGene (EcoliWiki Page)

EcoGene:EG10573

CGSC (EcoliWiki Page)

CGSC:10961

ASAP (EcoliWikiPage)

ASAP:ABE-0003891


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

McrA

Synonyms

e14 prophage; 5-methylcytosine-specific restriction endonuclease B[1], B1159[2][1], RglA[2][1], McrA[2][1]

Product description

restriction of DNA at 5-methylcytosine residues[2][3]

5-methylcytosine-specific restriction endonuclease B, e14; prophage gene[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0003676

nucleic acid binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002711

F

Seeded from EcoCyc [7]

complete

GO:0004518

nuclease activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc [7]

complete

GO:0004519

endonuclease activity

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002711
SP_KW:KW-0255

F

Seeded from EcoCyc [7]

complete

GO:0009307

DNA restriction-modification system

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0680

P

Seeded from EcoCyc [7]

complete

GO:0016787

hydrolase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1139015

Protein

nfnB

PMID:16606699

Experiment(s):EBI-1139015

Protein

rplB

PMID:16606699

Experiment(s):EBI-1139015

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MHVFDNNGIE LKAECSIGEE DGVYGLILES WGPGDRNKDY NIALDYIIER LVDSGVSQVV
VYLASSSVRK HMHSLDERKI HPGEYFTLIG NSPRDIRLKM CGYQAYFSRT GRKEIPSGNR
TKRILINVPG IYSDSFWASI IRGELSELSQ PTDDESLLNM RVSKLIKKTL SQPEGSRKPV
EVERLQKVYV RDPMVKAWIL QQSKGICENC GKNAPFYLND GNPYLEVHHV IPLSSGGADT
TDNCVALCPN CHRELHYSKN AKELIEMLYV NINRLQK
Length

277

Mol. Wt

31.39 kDa

pI

7.5 (calculated)

Extinction coefficient

35,870 - 36,745 (calc based on 13 Y, 3 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10573-MONOMER

UniProt (EcoliWiki Page)

UniProt:P24200

PFAM (EcoliWiki Page)

PFAM:PF01844

RefSeq (EcoliWiki Page)

RefSeq:NP_415677


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mcrA

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=1209549..1209589&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b1159 (EcoliWiki Page)

NCBI GEO profiles for mcrA

microarray

GenExpDB:b1159 (EcoliWiki Page)

Summary of data for mcrA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (1209377..1209647) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ07; Well:B3[8]

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Notes

Accessions Related to mcrA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10573

EchoBASE (EcoliWiki Page)

EchoBASE:EB0568

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1159

EcoGene (EcoliWiki Page)

EcoGene:EG10573


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Notes

Links

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Evolution

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Homologs in Other Organisms

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01844


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

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