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mcrB:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
McrB product |
|---|---|
| Synonyms |
5-methylcytosine-specific restriction enzyme McrBC, subunit McrB product[1], B4346 product[2][1], RglB product[2][1], McrB product[2][1] |
| Product description |
McrB subunit of 5-methylcytosine restriction system[2][3]; Component of McrBC restriction endonuclease[3] McrBC restriction endonuclease, catalytic subunit; 5-methylcytosine-specific; requires GTP, inhibited by ATP[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0003677 |
DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0004518 |
nuclease activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0004519 |
endonuclease activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0005515 |
protein binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005524 |
ATP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0005525 |
GTP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0009307 |
DNA restriction-modification system |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [7] |
complete | |||
| GO:0009376 |
HslUV protease complex |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc [7] |
complete | |||
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0016887 |
ATPase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0017111 |
nucleoside-triphosphatase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0005737 |
cytoplasm |
C |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0070011 |
peptidase activity, acting on L-amino acid peptides |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [7] |
complete | |||
| GO:0006308 |
DNA catabolic process |
P |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of McrBC restriction endonuclease |
could be indirect | ||
|
Protein |
Experiment(s):EBI-891305 | |||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MRKAYLMESI QPWIEKFIKQ AQQQRSQSTK DYPTSYRNLR VKLSFGYGNF TSIPWFAFLG EGQEASNGIY PVILYYKDFD ELVLAYGISD TNEPHAQWQF SSDIPKTIAE YFQATSGVYP KKYGQSYYAC SQKVSQGIDY TRFASMLDNI INDYKLIFNS GKSVIPPMSK TESYCLEDAL NDLFIPETTI ETILKRLTIK KNIILQGPPG VGKTFVARRL AYLLTGEKAP QRVNMVQFHQ SYSYEDFIQG YRPNGVGFRR KDGIFYNFCQ QAKEQPEKKY IFIIDEINRA NLSKVFGEVM MLMEHDKRGE NWSVPLTYSE NDEERFYVPE NVYIIGLMNT ADRSLAVVDY ALRRRFSFID IEPGFDTPQF RNFLLNKKAE PSFVESLCQK MNELNQEISK EATILGKGFR IGHSYFCCGL EDGTSPDTQW LNEIVMTDIA PLLEEYFFDD PYKQQKWTNK LLGDS |
|---|---|
| Length |
465 |
| Mol. Wt |
53.922 kDa |
| pI |
5.7 (calculated) |
| Extinction coefficient |
76,210 - 76,960 (calc based on 29 Y, 6 W, and 6 C residues) |
| edit table |
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
- ↑ 6.0 6.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
