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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

mdtN

Gene Synonym(s)

yjcR, ECK4075, b4082, JW4043[1][2]

Product Desc.

predicted membrane fusion protein of efflux pump[2][3]

Putative membrane fusion protein (MFP) of tripartite MdtNOP efflux pump, signal anchor; mdtP mutants are sensitive to acriflavin and puromycin[4]

Product Synonyms(s)

predicted membrane fusion protein of efflux pump[1], B4082[2][1], MdtN[2][1], YjcR[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yjcP[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(mdtN)    Textpresso(mdtN)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mdtN

Mnemonic

Multidrug transporter

Synonyms

yjcR, ECK4075, b4082, JW4043[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

92.7 minutes 

MG1655: 4302132..4301101
gbrowse_img.php?coord=4301101..4302132&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4281900..4282931
gbrowse_img.php?coord=4281900..4282931&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4307588..4306557
gbrowse_img.php?coord=4306557..4307588&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 4239836..4240867
gbrowse_img.php?coord=4239836..4240867&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔmdtN777::kan

PMID:16738554

CGSC:106240


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11954

EchoBASE (EcoliWiki Page)

EchoBASE:EB1897

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4082

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5087

EcoGene (EcoliWiki Page)

EcoGene:EG11954

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YJCR

ASAP (EcoliWikiPage)

ASAP:ABE-0013373


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YjcR

Synonyms

predicted membrane fusion protein of efflux pump[1], B4082[2][1], MdtN[2][1], YjcR[2][1]

Product description

predicted membrane fusion protein of efflux pump[2][3]

Putative membrane fusion protein (MFP) of tripartite MdtNOP efflux pump, signal anchor; mdtP mutants are sensitive to acriflavin and puromycin[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [5]

complete

GO:0006855

multidrug transport

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005694

P

Seeded from EcoCyc [5]

complete

GO:0008565

protein transporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006143

F

Seeded from EcoCyc [5]

complete

GO:0009276

Gram-negative-bacterium-type cell wall

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005694

C

Seeded from EcoCyc [5]

complete

GO:0009306

protein secretion

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006143

P

Seeded from EcoCyc [5]

complete

GO:0015238

drug transporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005694

F

Seeded from EcoCyc [5]

complete

GO:0016020

membrane

GO_REF:0000002
GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR006143
SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0016021

integral to membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc [5]

complete

GO:0046677

response to antibiotic

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc [5]

complete

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [5]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

rluC

PMID:16606699

Experiment(s):EBI-1147418

Protein

rplB

PMID:16606699

Experiment(s):EBI-1147418

Protein

rpsC

PMID:16606699

Experiment(s):EBI-1147418

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MESTPKKAPR SKFPALLVVA LALVALVFVI WRVDSAPSTN DAYASADTID VVPEVSGRIV
ELAVTDNQAV KQGDLLFRID PRPYEANLAK AEASLAALDK QIMLTQRSVD AQQFGADSVN
ATVEKARAAA KQATDTLRRT EPLLKEGFVS AEDVDRARTA QRAAEADLNA VLLQAQSAAS
AVSGVDALVA QRAAVEADIA LTKLHLEMAT VRAPFDGRVI SLKTSVGQFA SAMRPIFTLI
DTRHWYVIAN FRETDLKNIR SGTPATIRLM SDSGKTFEGK VDSIGYGVLP DDGGLVLGGL
PKVSRSINWV RVAQRFPVKI MVDKPDPEMF RIGASAVANL EPQ
Length

343

Mol. Wt

36.908 kDa

pI

7.2 (calculated)

Extinction coefficient

22,460 (calc based on 4 Y, 3 W, and C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11954-MONOMER

UniProt (EcoliWiki Page)

UniProt:P32716

PFAM (EcoliWiki Page)

PFAM:PF00529

RefSeq (EcoliWiki Page)

RefSeq:NP_418506

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YJCR-MONOMER


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yjcP

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=4302112..4302152&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b4082 (EcoliWiki Page)

NCBI GEO profiles for mdtN

microarray

GenExpDB:b4082 (EcoliWiki Page)

Summary of data for mdtN from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (4301591..4302247) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ03; Well:B8[7]

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Notes

Accessions Related to mdtN Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11954

EchoBASE (EcoliWiki Page)

EchoBASE:EB1897

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4082

EcoGene (EcoliWiki Page)

EcoGene:EG11954


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

YJCR

From SHIGELLACYC

E. coli O157

YJCR

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF00529


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  6. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

Categories

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