metB:Gene - EcoliWiki
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metB:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

metB

Mnemonic
Synonyms

ECK3931, b3939, JW3910, met-1[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

88.94 minutes, 88.94 minutes 

MG1655: 4126695..4127855
gbrowse_img.php?coord=4125695..4128855&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3508009..3506849
gbrowse_img.php?coord=3505849..3509009&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔmetB (Keio:JW3910)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10824[3]

metB1

PMID:14047235[4] PMID:16588691[5]

CGSC:4538

metB65

CGSC:6823

metB0

CGSC:9934

metB185

CGSC:10292

ΔmetB726::kan

PMID:16738554[2]

CGSC:101826

overexpression in a metC mutant

Growth Phenotype

Suppresses diaminopimelate requirement of dapA, dapB, and dapD

PMID:8262915[6]

meso-lanthionine and L-allo-cystathionine are produced and incorporated into peptidoglycan

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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW3910

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCACGCGTAAACAGGCCACCAT

Primer 2:CCgCCCTTGTTTGCAGCCCGGAA

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[3]

est. P1 cotransduction: 4% [8]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[3]

est. P1 cotransduction: 56% [8]

Synonyms:zij-501::Tn10


CAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10582

EchoBASE (EcoliWiki Page)

EchoBASE:EB0577

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3939

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4892

EcoGene (EcoliWiki Page)

EcoGene:EG10582

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:METB

CGSC (EcoliWiki Page)

CGSC:515

ASAP (EcoliWikiPage)

ASAP:ABE-0012887


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. PITTARD J et al. (1963) GENE TRANSFER BY F' STRAINS OF ESCHERICHIA COLI K-12. I. DELAY IN INITIATION OF CHROMOSOME TRANSFER. J Bacteriol 85: 1394-401 PubMed EcoliWiki page
  5. Tatum EL (1945) X-Ray Induced Mutant Strains of Escherichia Coli. Proc Natl Acad Sci U S A 31: 215-9 PubMed EcoliWiki page
  6. Richaud C et al. (1993) Directed evolution of biosynthetic pathways. Recruitment of cysteine thioethers for constructing the cell wall of Escherichia coli. J Biol Chem 268: 26827-35 PubMed EcoliWiki page
  7. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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