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metH:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MetH

Synonyms

homocysteine-N5-methyltetrahydrofolate transmethylase, B12-dependent[1], B4019[2][1], MetH[2][1]

Product description

Methionine synthase, cobalamin-dependent; 5-methyltetrahydrofolate-homocysteine methyltransferase; binds Zn(II)[3]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0004156

dihydropteroate synthase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000489

F

Seeded from EcoCyc [4]

complete

GO:0005622

intracellular

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004223

C

Seeded from EcoCyc [4]

complete

GO:0008168

methyltransferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0489

F

Seeded from EcoCyc [4]

complete

GO:0008270

zinc ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc [4]

complete

GO:0008652

amino acid biosynthetic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc [4]

complete

GO:0008705

methionine synthase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR003759
InterPro:IPR004223
InterPro:IPR011822
EC:2.1.1.13

F

Seeded from EcoCyc [4]

complete

GO:0008898

homocysteine S-methyltransferase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003726

F

Seeded from EcoCyc [4]

complete

GO:0009086

methionine biosynthetic process

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003759
InterPro:IPR004223
InterPro:IPR011822
SP_KW:KW-0486

P

Seeded from EcoCyc [4]

complete

GO:0009396

folic acid and derivative biosynthetic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000489

P

Seeded from EcoCyc [4]

complete

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [4]

complete

GO:0031419

cobalamin binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003759
InterPro:IPR006158
InterPro:IPR011822
SP_KW:KW-0846

F

Seeded from EcoCyc [4]

complete

GO:0044237

cellular metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011005

P

Seeded from EcoCyc [4]

complete

GO:0046872

metal ion binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003759
InterPro:IPR006158
SP_KW:KW-0479

F

Seeded from EcoCyc [4]

complete

GO:0050897

cobalt ion binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR011822
SP_KW:KW-0170

F

Seeded from EcoCyc [4]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0006556

S-adenosylmethionine biosynthetic process

P

Seeded from EcoCyc 11.1[5].

required fields missing

GO:0009257

10-formyltetrahydrofolate biosynthetic process

P

Seeded from EcoCyc 11.1[5].

required fields missing

GO:0009236

cobalamin biosynthetic process

P

Seeded from EcoCyc 11.1[5].

required fields missing

GO:0005737

cytoplasm

PMID:9250984[6]

IDA: Inferred from Direct Assay

C

complete

GO:0005737

cytoplasm

PMID:2668277[7]

IDA: Inferred from Direct Assay

C

complete

GO:0008705

methionine synthase activity

PMID:10978155[8]

IDA: Inferred from Direct Assay

F

complete

GO:0008168

methyltransferase activity

Taylor and Weissbach (1971)[9]

IDA: Inferred from Direct Assay

F

complete

GO:0008898

homocysteine S-methyltransferase activity

Taylor and Weissbach (1971)[9]

IDA: Inferred from Direct Assay

F

complete

GO:0009257

10-formyltetrahydrofolate biosynthetic process

Taylor and Weissbach (1971)[9]

IDA: Inferred from Direct Assay

P

complete

GO:0008705

methionine synthase activity

Taylor and Weissbach (1971)[9]

IDA: Inferred from Direct Assay

F

complete

GO:0008652

amino acid biosynthetic process

Taylor and Weissbach (1971)[9]

IDA: Inferred from Direct Assay

P

complete

GO:0009086

methionine biosynthetic process

Taylor and Weissbach (1971)[9]

IDA: Inferred from Direct Assay

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

gltB

PMID:16606699[10]

Experiment(s):EBI-1147304

Protein

dnaE

PMID:16606699[10]

Experiment(s):EBI-1147304

Protein

ilvC

PMID:16606699[10]

Experiment(s):EBI-1147304

Protein

bglX

PMID:16606699[10]

Experiment(s):EBI-1147304

Small Molecule

Cobalamin

PMID:9201956[11]


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MSSKVEQLRA QLNERILVLD GGMGTMIQSY RLNEADFRGE RFADWPCDLK GNNDLLVLSK
PEVIAAIHNA YFEAGADIIE TNTFNSTTIA MADYQMESLS AEINFAAAKL ARACADEWTA
RTPEKPRYVA GVLGPTNRTA SISPDVNDPA FRNITFDGLV AAYRESTKAL VEGGADLILI
ETVFDTLNAK AAVFAVKTEF EALGVELPIM ISGTITDASG RTLSGQTTEA FYNSLRHAEA
LTFGLNCALG PDELRQYVQE LSRIAECYVT AHPNAGLPNA FGEYDLDADT MAKQIREWAQ
AGFLNIVGGC CGTTPQHIAA MSRAVEGLAP RKLPEIPVAC RLSGLEPLNI GEDSLFVNVG
ERTNVTGSAK FKRLIKEEKY SEALDVARQQ VENGAQIIDI NMDEGMLDAE AAMVRFLNLI
AGEPDIARVP IMIDSSKWDV IEKGLKCIQG KGIVNSISMK EGVDAFIHHA KLLRRYGAAV
VVMAFDEQGQ ADTRARKIEI CRRAYKILTE EVGFPPEDII FDPNIFAVAT GIEEHNNYAQ
DFIGACEDIK RELPHALISG GVSNVSFSFR GNDPVREAIH AVFLYYAIRN GMDMGIVNAG
QLAIYDDLPA ELRDAVEDVI LNRRDDGTER LLELAEKYRG SKTDDTANAQ QAEWRSWEVN
KRLEYSLVKG ITEFIEQDTE EARQQATRPI EVIEGPLMDG MNVVGDLFGE GKMFLPQVVK
SARVMKQAVA YLEPFIEASK EQGKTNGKMV IATVKGDVHD IGKNIVGVVL QCNNYEIVDL
GVMVPAEKIL RTAKEVNADL IGLSGLITPS LDEMVNVAKE MERQGFTIPL LIGGATTSKA
HTAVKIEQNY SGPTVYVQNA SRTVGVVAAL LSDTQRDDFV ARTRKEYETV RIQHGRKKPR
TPPVTLEAAR DNDFAFDWQA YTPPVAHRLG VQEVEASIET LRNYIDWTPF FMTWSLAGKY
PRILEDEVVG VEAQRLFKDA NDMLDKLSAE KTLNPRGVVG LFPANRVGDD IEIYRDETRT
HVINVSHHLR QQTEKTGFAN YCLADFVAPK LSGKADYIGA FAVTGGLEED ALADAFEAQH
DDYNKIMVKA LADRLAEAFA EYLHERVRKV YWGYAPNENL SNEELIRENY QGIRPAPGYP
ACPEHTEKAT IWELLEVEKH TGMKLTESFA MWPGASVSGW YFSHPDSKYY AVAQIQRDQV
EDYARRKGMS VTEVERWLAP NLGYDAD
Length

1,227

Mol. Wt

135.998 kDa

pI

4.8 (calculated)

Extinction coefficient

136,600 - 138,225 (calc based on 40 Y, 14 W, and 13 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences

Initiator Methionine

1

Removed

UniProt:P13009


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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:HOMOCYSMETB12-MONOMER

UniProt (EcoliWiki Page)

UniProt:P13009

PFAM (EcoliWiki Page)

PFAM:PF02574

RefSeq (EcoliWiki Page)

RefSeq:NP_418443


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  4. ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  5. ↑ 5.0 5.1 5.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ Jarrett JT et al. (1997) Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli. Methods Enzymol 281: 196-213 PubMed EcoliWiki page
  7. ↑ Banerjee RV et al. (1989) Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain. J Biol Chem 264: 13888-95 PubMed EcoliWiki page
  8. ↑ Hall DA et al. (2000) Interaction of flavodoxin with cobalamin-dependent methionine synthase. Biochemistry 39: 10711-9 PubMed EcoliWiki page
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 Taylor, R.T. and Weissbach, H. NS-methyltetrahydrofolate-homocysteine (Vitamin B12) methyltransferase (Escherichia coli B). Methods Enzymol. 17B (1971) 379-388.Taylor & Weissbach
  10. ↑ 10.0 10.1 10.2 10.3 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
  11. ↑ Goulding CW et al. (1997) Cobalamin-dependent methionine synthase is a modular protein with distinct regions for binding homocysteine, methyltetrahydrofolate, cobalamin, and adenosylmethionine. Biochemistry 36: 8082-91 PubMed EcoliWiki page

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