metK:Gene Product(s)

Standard name	MetK
Synonyms	methionine adenosyltransferase 1^[1], B2942^[2]^[1], MetX^[2]^[1], MetK^[2]^[1]
Product description	MetK S-adenosylmethionine synthetase^[2]^[3]; Component of methionine adenosyltransferase I^[2]^[3] S-adenosylmethionine synthase; methionine adenosyltransferase; ethionine sensitivity; essential gene^[4]
EC number (for enzymes)	2.5.1.6^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc ^[5]	complete
	GO:0000287	magnesium ion binding	GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460 HAMAP:MF_00086	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0004478	methionine adenosyltransferase activity	GO_REF:0000002 GO_REF:0000003 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002133 EC:2.5.1.6 HAMAP:MF_00086	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005524	ATP binding	GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002133 SP_KW:KW-0067 HAMAP:MF_00086	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005737	cytoplasm	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0006730	one-carbon metabolic process	GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002133 SP_KW:KW-0554 HAMAP:MF_00086	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0016740	transferase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc ^[5]	complete
	GO:0030955	potassium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0630	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc ^[5]	complete
	GO:0050897	cobalt ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0170	F	Seeded from EcoCyc ^[5]	complete
	GO:0005829	cytosol	PMID:16858726^[7]	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc ^[5]	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of methionine adenosyltransferase I	could be indirect
Protein	fdnH		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	prs		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	ybfC		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	groL		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	nadE		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	phnN		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	speB		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	degQ		PMID:16606699^[8]	Experiment(s):EBI-1144372
Protein	dnaK		PMID:15690043^[9]	Experiment(s):EBI-881611
Protein	glyQ		PMID:15690043^[9]	Experiment(s):EBI-881611
Protein	mreB		PMID:15690043^[9]	Experiment(s):EBI-881611
Protein	pstB		PMID:15690043^[9]	Experiment(s):EBI-881611
Protein	recA		PMID:15690043^[9]	Experiment(s):EBI-881611
Protein	secA		PMID:15690043^[9]	Experiment(s):EBI-881611, EBI-880477
Protein	tufA		PMID:15690043^[9]	Experiment(s):EBI-881611, EBI-889981, EBI-889076
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Notes

Localization

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Compartment	Description	Evidence	Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MAKHLFTSES VSEGHPDKIA DQISDAVLDA ILEQDPKARV ACETYVKTGM VLVGGEITTS AWVDIEEITR NTVREIGYVH SDMGFDANSC AVLSAIGKQS PDINQGVDRA DPLEQGAGDQ GLMFGYATNE TDVLMPAPIT YAHRLVQRQA EVRKNGTLPW LRPDAKSQVT FQYDDGKIVG IDAVVLSTQH SEEIDQKSLQ EAVMEEIIKP ILPAEWLTSA TKFFINPTGR FVIGGPMGDC GLTGRKIIVD TYGGMARHGG GAFSGKDPSK VDRSAAYAAR YVAKNIVAAG LADRCEIQVS YAIGVAEPTS IMVETFGTEK VPSEQLTLLV REFFDLRPYG LIQMLDLLHP IYKETAAYGH FGREHFPWEK TDKAQLLRDA AGLK
Length	384
Mol. Wt	41.951 kDa
pI	5.0 (calculated)
Extinction coefficient	39,880 - 40,380 (calc based on 12 Y, 4 W, and 4 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A817
Modification Site	198	phosphorylation site at S198	probability greater than 75%	PMID:17938405^[10]
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Structure
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Structures

Representative Escherichia coli structure:
1FUG|A
1-383 of 384 residues (E-value: 9e-206) (Percent Identity: 100.00)
View the PDB Table for metK

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:S-ADENMETSYN-MONOMER
UniProt (EcoliWiki Page)	UniProt:P0A817
PFAM (EcoliWiki Page)	PFAM:PF00438
RefSeq (EcoliWiki Page)	RefSeq:NP_417417
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Lasserre JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27: 3306-21 PubMed EcoliWiki page
↑ ^8.0 ^8.1 ^8.2 ^8.3 ^8.4 ^8.5 ^8.6 ^8.7 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ ^9.0 ^9.1 ^9.2 ^9.3 ^9.4 ^9.5 ^9.6 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
↑ Macek B et al. (2007) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol Cell Proteomics PubMed EcoliWiki page

metK:Gene Product(s)

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Nomenclature

Notes

Function

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Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

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Accessions in Other Databases

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Links

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