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mgtA:On One Page
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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| Standard Name |
mgtA |
|---|---|
| Gene Synonym(s) | |
| Product Desc. |
MgtA[2]; magnesium transporter[3] Magnesium transporter, ATP-dependent; mutant has cobalt resistance; mediates Mg(2+) influx[4] |
| Product Synonyms(s) |
magnesium transporter product[1], B4242 product[2][1], Mtg product[2][1], Mgt product[2][1], CorB product[2][1], AtmA product[2][1], MgtA product[2][1] |
| Function from GO |
Molecular Function |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links | |
| edit table |
See Help:Quickview for help with entering information in the Quickview table.
Notes
Mg(2+) stimulon-PhoPQ regulon, EvgAS-PhoPQ cascade enhanced-expression subset. Salmonella mgtA is regulated by a Mg(2+)-responsive riboswitch; the 5' UTR secondary structure is conserved in E. coli, indicating E. coli mgtA should also have riboswitch regulation. HAD superfamily. MgtA contains a putative phosphatase HAD domain: residues 367-662.[4]
Gene
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
mgtA |
|---|---|
| Mnemonic |
Magnesium transport |
| Synonyms | |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
96.25 minutes |
MG1655: 4465648..4468344 | ||
|
NC_012967: 4450902..4453598 | ||||
|
W3110 |
|
W3110: 4472305..4475001 | ||
|
DH10B: 4567456..4570152 | ||||
|
NC_012759: 4404383..4407079 | ||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature type | Location | Description |
|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔmgtA (Keio:JW4201) |
deletion |
deletion |
PMID:16738554 | ||||
|
ΔmgtA789::kan |
PMID:16738554 |
| |||||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW4201 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:gccTTTAAAGAAATTTTTACCCGGCTCATTCGC Primer 2:ccTTGCCAGCCGTAACGACGGCTATAGAAC | |
|
Linked marker |
est. P1 cotransduction: 47% [6] | ||
|
Linked marker |
est. P1 cotransduction: % [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Product(s)
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
MgtA product |
|---|---|
| Synonyms |
magnesium transporter product[1], B4242 product[2][1], Mtg product[2][1], Mgt product[2][1], CorB product[2][1], AtmA product[2][1], MgtA product[2][1] |
| Product description |
MgtA[2]; magnesium transporter[3] Magnesium transporter, ATP-dependent; mutant has cobalt resistance; mediates Mg(2+) influx[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
GO:0005515 |
protein binding |
PMID:16606699 |
IPI: Inferred from Physical Interaction |
UniProtKB:P09373 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0000166 |
nucleotide binding |
GO_REF:0000004 |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0000287 |
magnesium ion binding |
GO_REF:0000004 |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0460 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0003824 |
catalytic activity |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005834 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0005524 |
ATP binding |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
F |
Seeded from EcoCyc 12.5 [8] |
complete | |
|
GO:0006810 |
transport |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001757 |
P |
Seeded from EcoCyc 12.5 [8] |
complete | |
|
GO:0006812 |
cation transport |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004014 |
P |
Seeded from EcoCyc 12.5 [8] |
complete | |
|
GO:0008152 |
metabolic process |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005834 |
P |
Seeded from EcoCyc [7] |
complete | |
|
GO:0015444 |
magnesium-importing ATPase activity |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006415 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0015662 |
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
F |
Seeded from EcoCyc 12.5 [8] |
complete | |
|
GO:0015693 |
magnesium ion transport |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006415 |
P |
Seeded from EcoCyc [7] |
complete | |
|
GO:0015992 |
proton transport |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
P |
Seeded from EcoCyc 12.5 [8] |
complete | |
|
GO:0016020 |
membrane |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000695 |
C |
Seeded from EcoCyc 12.5 [8] |
complete | |
|
GO:0016021 |
integral to membrane |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006415 |
C |
Seeded from EcoCyc [7] |
complete | |
|
GO:0016787 |
hydrolase activity |
GO_REF:0000004 |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0378 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0016820 |
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR008250 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0046872 |
metal ion binding |
GO_REF:0000004 |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0479 |
F |
Seeded from EcoCyc [7] |
complete | |
|
GO:0005886 |
plasma membrane |
GO_REF:0000004 |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0997 |
C |
Seeded from EcoCyc [7] |
complete | |
|
GO:0006754 |
ATP biosynthetic process |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001757 |
P |
Seeded from EcoCyc [7] |
complete | |
|
GO:0015986 |
ATP synthesis coupled proton transport |
P |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
pflB |
PMID:16606699 |
Experiment(s):EBI-1147754 | |
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
|
plasma membrane |
From EcoCyc[3] |
| ||
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MFKEIFTRLI RHLPSRLVHR DPLPGAQQTV NTVVPPSLSA HCLKMAVMPE EELWKTFDTH PEGLNQAEVE SAREQHGENK LPAQQPSPWW VHLWVCYRNP FNILLTILGA ISYATEDLFA AGVIALMVAI STLLNFIQEA RSTKAADALK AMVSNTATVL RVINDKGENG WLEIPIDQLV PGDIIKLAAG DMIPADLRIL QARDLFVAQA SLTGESLPVE KAATTRQPEH SNPLECDTLC FMGTTVVSGT AQAMVIATGA NTWFGQLAGR VSEQESEPNA FQQGISRVSM LLIRFMLVMA PVVLLINGYT KGDWWEAALF ALSVAVGLTP EMLPMIVTST LARGAVKLSK QKVIVKHLDA IQNFGAMDIL CTDKTGTLTQ DKIVLENHTD ISGKTSERVL HSAWLNSHYQ TGLKNLLDTA VLEGTDEESA RSLASRWQKI DEIPFDFERR RMSVVVAENT EHHQLVCKGA LQEILNVCSQ VRHNGEIVPL DDIMLRKIKR VTDTLNRQGL RVVAVATKYL PAREGDYQRA DESDLILEGY IAFLDPPKET TAPALKALKA SGITVKILTG DSELVAAKVC HEVGLDAGEV VIGSDIETLS DDELANLAQR TTLFARLTPM HKERIVTLLK REGHVVGFMG DGINDAPALR AADIGISVDG AVDIAREAAD IILLEKSLMV LEEGVIEGRR TFANMLKYIK MTASSNFGNV FSVLVASAFL PFLPMLPLHL LIQNLLYDVS QVAIPFDNVD DEQIQKPQRW NPADLGRFMI FFGPISSIFD ILTFCLMWWV FHANTPETQT LFQSGWFVVG LLSQTLIVHM IRTRRVPFIQ SCASWPLMIM TVIVMIVGIA LPFSPLASYL QLQALPLSYF PWLVAILAGY MTLTQLVKGF YSRRYGWQ |
|---|---|
| Length |
898 |
| Mol. Wt |
99.467 kDa |
| pI |
5.9 (calculated) |
| Extinction coefficient |
114,360 - 115,610 (calc based on 14 Y, 17 W, and 10 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Expression
Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Cellular level | Units | Medium | Temperature °C | Other (e.g. anaerobic) | Growth rate | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|---|---|---|
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Notes
Transcription and Transcriptional Regulation
|
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
| Figure courtesy of RegulonDB |
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
This picture shows the sequence around the N-terminus.
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for mgtA | |
|
microarray |
Summary of data for mgtA from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Accessions Related to mgtA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Evolution
Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
| Organism | Homologs (Statistics) | Comments |
|---|---|---|
|
Anopheles gambiae |
|
From Inparanoid:20070104 |
|
Apis mellifera |
|
From Inparanoid:20070104 |
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Arabidopsis thaliana |
|
From Inparanoid:20070104 |
|
Bos taurus |
|
From Inparanoid:20070104 |
|
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
|
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
|
Canis familiaris |
|
From Inparanoid:20070104 |
|
Ciona intestinalis |
|
From Inparanoid:20070104 |
|
Danio rerio |
|
From Inparanoid:20070104 |
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Dictyostelium discoideum |
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From Inparanoid:20070104 |
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Drosophila melanogaster |
|
From Inparanoid:20070104 |
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Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
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Gallus gallus |
|
From Inparanoid:20070104 |
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Homo sapiens |
|
From Inparanoid:20070104 |
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Macaca mulatta |
|
From Inparanoid:20070104 |
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Monodelphis domestica |
|
From Inparanoid:20070104 |
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Mus musculus |
|
From Inparanoid:20070104 |
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Oryza gramene |
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From Inparanoid:20070104 |
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Pan troglodytes |
|
From Inparanoid:20070104 |
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Rattus norvegicus |
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From Inparanoid:20070104 |
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Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
|
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
|
Takifugu rubripes |
|
From Inparanoid:20070104 |
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Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
|
Xenopus tropicalis |
|
From Inparanoid:20070104 |
|
Shigella flexneri |
MGTA |
From SHIGELLACYC |
|
E. coli O157 |
MGTA |
From ECOO157CYC |
| edit table |
Do-It-Yourself Web Tools
Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 8.0 8.1 8.2 8.3 8.4 8.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
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