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moaD:On One Page

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Standard Name

moaD

Gene Synonym(s)

ECK0773, b0784, JW0767, chlM, chlA4[1]

Product Desc.

molybdopterin synthase small subunit[2][3];

Component of molybdopterin synthase[3]

Molybdopterin (MPT) synthase, small subunit; chlorate resistance; dimer of dimers with MoaE[4]

Product Synonyms(s)

molybdopterin synthase, small subunit[1], B0784[2][1], ChlM[2][1], MoaD[2][1]

Function from GO

Molecular Function

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): moaBCDE[2], moaABCDE[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(moaD)    Textpresso(moaD)

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Notes

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The C-terminus of MoaD is thiocarboxylated.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

moaD

Mnemonic

Molybdenate

Synonyms

ECK0773, b0784, JW0767, chlM, chlA4[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

17.64 minutes 

MG1655: 818271..818516
gbrowse_img.php?coord=818271..818516&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 811674..811919
gbrowse_img.php?coord=811674..811919&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 819470..819715
gbrowse_img.php?coord=819470..819715&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 872199..872444
gbrowse_img.php?coord=872199..872444&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 721239..721484
gbrowse_img.php?coord=721239..721484&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔmoaD (Keio:JW0767)

deletion

deletion

PMID:16738554

Shigen
CGSC8824[5]

moaD

PMID:12519969

ASAP

At position 107 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20238

ΔmoaD756::kan

PMID:16738554

CGSC:101904


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW0767

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCATTAAAGTTCTTTTTTTCGC

Primer 2:CCACCTCCGGTTACCGGCGGGAA

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[5]

est. P1 cotransduction: 20% [6]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[5]

est. P1 cotransduction: 91% [6]
Synonyms:zbi-29::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11597

EchoBASE (EcoliWiki Page)

EchoBASE:EB1554

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0784

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0866

EcoGene (EcoliWiki Page)

EcoGene:EG11597

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MOAD

CGSC (EcoliWiki Page)

CGSC:18475

ASAP (EcoliWikiPage)

ASAP:ABE-0002675


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MoaD

Synonyms

molybdopterin synthase, small subunit[1], B0784[2][1], ChlM[2][1], MoaD[2][1]

Product description

molybdopterin synthase small subunit[2][3];

Component of molybdopterin synthase[3]

Molybdopterin (MPT) synthase, small subunit; chlorate resistance; dimer of dimers with MoaE[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0005515

protein binding

PMID:11713534

IPI: Inferred from Physical Interaction

UniProtKB:P12282

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P0A7K2

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P30749

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0006777

Mo-molybdopterin cofactor biosynthetic process

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR010034
SP_KW:KW-0501

P

Seeded from EcoCyc [8]

complete

GO:0006790

sulfur metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003749

P

Seeded from EcoCyc [8]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of molybdopterin synthase

could be indirect

Protein

moaC

PMID:16606699

Experiment(s):EBI-1137971

Protein

rplF

PMID:16606699

Experiment(s):EBI-1137971

Protein

lon

PMID:16606699

Experiment(s):EBI-1137971

Protein

yjhQ

PMID:16606699

Experiment(s):EBI-1137971

Protein

insK

PMID:16606699

Experiment(s):EBI-1137971

Protein

nadE

PMID:16606699

Experiment(s):EBI-1137971

Protein

dadA

PMID:16606699

Experiment(s):EBI-1137971

Protein

ydcP

PMID:16606699

Experiment(s):EBI-1137971

Protein

cheA

PMID:16606699

Experiment(s):EBI-1137971

Protein

glyQ

PMID:16606699

Experiment(s):EBI-1137971

Protein

rplL

PMID:15690043

Experiment(s):EBI-889969

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MIKVLFFAQV RELVGTDATE VAADFPTVEA LRQHMAAQSD RWALALEDGK LLAAVNQTLV
SFDHPLTDGD EVAFFPPVTG G
Length

81

Mol. Wt

8.757 kDa

pI

4.3 (calculated)

Extinction coefficient

5,500 (calc based on Y, 1 W, and C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11597-MONOMER

UniProt (EcoliWiki Page)

UniProt:P30748

PFAM (EcoliWiki Page)

PFAM:PF02597

RefSeq (EcoliWiki Page)

RefSeq:NP_415305


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

moaBCDE

moaABCDE

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=818251..818291&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b0784 (EcoliWiki Page)

NCBI GEO profiles for moaD

microarray

GenExpDB:b0784 (EcoliWiki Page)

Summary of data for moaD from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to moaD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11597

EchoBASE (EcoliWiki Page)

EchoBASE:EB1554

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0784

EcoGene (EcoliWiki Page)

EcoGene:EG11597


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Shigella flexneri

MOAD

From SHIGELLACYC

E. coli O157

MOAD

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF02597


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.0 8.1 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

Categories

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