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mprA:On One Page

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Standard Name

mprA

Gene Synonym(s)

ECK2678, b2684, JW2659, emrR[1][2]

Product Desc.

EmrR transcriptional repressor[3]; MprA transcriptional repressor[2]

emrAB repressor, also regulates microcin synthesis; binds uncouplers DNP, CCCP, FCCP[4]

Product Synonyms(s)

DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux[1], B2684[3][1], MprA[3][1], EmrR[3][1]

Function from GO

Biological Process

Molecular Function

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): emrRAB[3]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(mprA)    Textpresso(mprA)

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Notes

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Gene

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Nomenclature

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Standard name

mprA

Mnemonic

Microcin peptide regulation

Synonyms

ECK2678, b2684, JW2659, emrR[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

60.54 minutes 

MG1655: 2808792..2809322
gbrowse_img.php?coord=2808792..2809322&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2809426..2809956
gbrowse_img.php?coord=2809426..2809956&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 2694604..2695134
gbrowse_img.php?coord=2694604..2695134&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔmprA (Keio:JW2659)

deletion

deletion

PMID:16738554

Shigen
CGSC10097[5]

emrR::Tn5KAN

PMID:12519969

ASAP

At position 422 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20880

emrR::Tn5KAN

PMID:12519969

ASAP

At position 422 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20881

ΔmprA765::kan

PMID:16738554

CGSC:101938


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW2659

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGATAGTTCGTTTACGCCCAT

Primer 2:CCGCTCATCGCTTCGAGAACCAC

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[5]

est. P1 cotransduction: 1% [6]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: 55% [6]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10603

EchoBASE (EcoliWiki Page)

EchoBASE:EB0598

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2684

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3237

EcoGene (EcoliWiki Page)

EcoGene:EG10603

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:EMRR

CGSC (EcoliWiki Page)

CGSC:33255

ASAP (EcoliWikiPage)

ASAP:ABE-0008834


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MprA

Synonyms

DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux[1], B2684[3][1], MprA[3][1], EmrR[3][1]

Product description

EmrR transcriptional repressor[3]; MprA transcriptional repressor[2]

emrAB repressor, also regulates microcin synthesis; binds uncouplers DNP, CCCP, FCCP[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0006350

transcription

PMID:10438794

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0006350

transcription

PMID:10991887

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0016564

transcription repressor activity

PMID:10438794

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0016564

transcription repressor activity

PMID:10991887

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [7]

complete

GO:0003677

DNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc [8]

complete

GO:0003700

transcription factor activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000835

F

Seeded from EcoCyc [8]

complete

GO:0005622

intracellular

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000835

C

Seeded from EcoCyc [8]

complete

GO:0006350

transcription

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc 12.5 [7]

complete

GO:0006355

regulation of transcription, DNA-dependent

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000835
SP_KW:KW-0805

P

Seeded from EcoCyc [8]

complete

GO:0045892

negative regulation of transcription, DNA-dependent

P

Seeded from EcoCyc 11.1[2].

required fields missing

GO:0046677

response to antibiotic

P

Seeded from EcoCyc 11.1[2].

required fields missing

GO:0046678

response to bacteriocin

P

Seeded from EcoCyc 11.1[2].

required fields missing

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[2].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

lacZ

PMID:16606699

Experiment(s):EBI-1143661

Protein

glgB

PMID:16606699

Experiment(s):EBI-1143661

Protein

hchA

PMID:16606699

Experiment(s):EBI-1143661

Protein

narZ

PMID:16606699

Experiment(s):EBI-1143661

Protein

perR

PMID:16606699

Experiment(s):EBI-1143661

Protein

fusA

PMID:16606699

Experiment(s):EBI-1143661

Protein

yedZ

PMID:16606699

Experiment(s):EBI-1143661

Protein

hofH

PMID:16606699

Experiment(s):EBI-1143661

Protein

nuoG

PMID:16606699

Experiment(s):EBI-1143661

Protein

alaS

PMID:16606699

Experiment(s):EBI-1143661

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MDSSFTPIEQ MLKFRASRHE DFPYQEILLT RLCMHMQSKL LENRNKMLKA QGINETLFMA
LITLESQENH SIQPSELSCA LGSSRTNATR IADELEKRGW IERRESDNDR RCLHLQLTEK
GHEFLREVLP PQHNCLHQLW SALSTTEKDQ LEQITRKLLS RLDQMEQDGV VLEAMS
Length

176

Mol. Wt

20.563 kDa

pI

6.2 (calculated)

Extinction coefficient

12,490 - 12,990 (calc based on 1 Y, 2 W, and 4 C residues)

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Domains/Motifs/Modification Sites

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TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10603-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0ACR9

PFAM (EcoliWiki Page)

PFAM:PF01047

RefSeq (EcoliWiki Page)

RefSeq:NP_417169


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

emrRAB

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File:.jpg

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2808772..2808812&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2684 (EcoliWiki Page)

NCBI GEO profiles for mprA

microarray

GenExpDB:b2684 (EcoliWiki Page)

Summary of data for mprA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (2808626..2808839) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ02; Well:D8[9]

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Notes

Accessions Related to mprA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10603

EchoBASE (EcoliWiki Page)

EchoBASE:EB0598

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2684

EcoGene (EcoliWiki Page)

EcoGene:EG10603


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Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Xenopus tropicalis

  • ENSXETP00000000552 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

EMRR

From SHIGELLACYC

E. coli O157

EMRR

From ECOO157CYC

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Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01047


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  8. ↑ 8.0 8.1 8.2 8.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  9. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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