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mutS:Gene Product(s)
From EcoliWiki
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
MutS |
|---|---|
| Synonyms |
methyl-directed mismatch repair protein[1], B2733[2][1], Plm[2][1], Ant[2][1], Fdv[2][1], MutS[2][1] |
| Product description |
Component of MutHLS complex, methyl-directed mismatch repair[2][3] Methyl-directed mismatch repair protein; dimeric/tetrameric[4] |
| EC number (for enzymes) |
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| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006298 |
mismatch repair |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0030983 |
mismatched DNA binding |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc [10] |
complete | |||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0032300 |
mismatch repair complex |
IGI: Inferred from Genetic Interaction |
C |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [10] |
complete | |||
| GO:0003677 |
DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc [10] |
complete | |||
| GO:0003684 |
damaged DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |||
| GO:0005524 |
ATP binding |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000432 |
F |
Seeded from EcoCyc 12.5 [6] |
complete | |
| GO:0006281 |
DNA repair |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [10] |
complete | |||
| GO:0006298 |
mismatch repair |
GO_REF:0000002 |
IEA: Inferred from Electronic Annotation |
InterPro:IPR000432 |
P |
Seeded from EcoCyc 12.5 [6] |
complete | |
| GO:0006974 |
response to DNA damage stimulus |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc [10] |
complete | |||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc [10] |
complete | |||
| GO:0005737 |
cytoplasm |
C |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0006298 |
mismatch repair |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0030983 |
mismatched DNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006298 |
mismatch repair |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0016887 |
ATPase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0032300 |
mismatch repair complex |
IGI: Inferred from Genetic Interaction |
C |
P06722 (MutH) P23367 (MutL) |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of MutHLS complex, methyl-directed mismatch repair |
Inferred from genetic interaction | ||
|
Protein |
Experiment(s):EBI-886447 | |||
|
Protein |
Experiment(s):EBI-1143773 | |||
|
Protein |
Experiment(s):EBI-1143773 | |||
|
Protein |
Experiment(s):EBI-1143773 | |||
|
Protein |
DnaN: beta sliding clamp subunit of DNA pol III |
Protein mobility shift during native gel electrophoresis and kinase protection assay. | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL LDISLTKRGA SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA TSKGPVERKV VRIVTPGTIS DEALLQERQD NLLAAIWQDS KGFGYATLDI SSGRFRLSEP ADRETMAAEL QRTNPAELLY AEDFAEMSLI EGRRGLRRRP LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL LQYAKDTQRT TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER ILARLALRTA RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF AELRDLLERA IIDTPPVLVR DGGVIASGYN EELDEWRALA DGATDYLERL EVRERERTGL DTLKVGFNAV HGYYIQISRG QSHLAPINYM RRQTLKNAER YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE ALQQSASALA ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI DRIFTRVGAA DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS TYDGLSLAWA CAENLANKIK ALTLFATHYF ELTQLPEKME GVANVHLDAL EHGDTIAFMH SVQDGAASKS YGLAVAALAG VPKEVIKRAR QKLRELESIS PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR QALEWIYRLK SLV |
|---|---|
| Length |
853 |
| Mol. Wt |
95.249 kDa |
| pI |
5.4 (calculated) |
| Extinction coefficient |
73,230 - 73,980 (calc based on 27 Y, 6 W, and 6 C residues) |
| edit table |
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures
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| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 Glickman BW & Radman M (1980) Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc Natl Acad Sci U S A 77: 1063-7 PubMed EcoliWiki page
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 7.0 7.1 Su SS & Modrich P (1986) Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs. Proc Natl Acad Sci U S A 83: 5057-61 PubMed EcoliWiki page
- ↑ 8.0 8.1 Lahue RS et al. (1989) DNA mismatch correction in a defined system. Science 245: 160-4 PubMed EcoliWiki page
- ↑ 9.0 9.1 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
- ↑ 10.0 10.1 10.2 10.3 10.4 10.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
- ↑ 11.0 11.1 11.2 Bjornson KP et al. (2000) Modulation of MutS ATP hydrolysis by DNA cofactors. Biochemistry 39: 3176-83 PubMed EcoliWiki page
- ↑ 12.0 12.1 12.2 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
- ↑ López de Saro FJ & O'Donnell M (2001) Interaction of the beta sliding clamp with MutS, ligase, and DNA polymerase I. Proc Natl Acad Sci U S A 98: 8376-80 PubMed EcoliWiki page
