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mutS:On One Page

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Standard Name

mutS

Gene Synonym(s)

ECK2728, b2733, JW2703, fdv, ant, plm[1]

Product Desc.

MutS[2][3];

Component of MutHLS complex, methyl-directed mismatch repair[2][3]

Methyl-directed mismatch repair protein; dimeric/tetrameric[4]

Product Synonyms(s)

methyl-directed mismatch repair protein[1], B2733[2][1], Plm[2][1], Ant[2][1], Fdv[2][1], MutS[2][1]

Function from GO

Biological Process

Molecular Function

Cellular Component

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mutS[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(mutS)    Textpresso(mutS)

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Notes

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MutS, also known as the "mismatch recognition" enzyme, is essential for the DNA mismatch repair (MMR) biological pathway. It recognizes base-base mismatches and small nucleotide insertion/deletion mispairs generated during DNA synthesis or damage caused by various agents. After recognizing the mismatches, mutS binds to the mismatch as a homodimer. Although the mutS binding site is sequence-wise identical, the mismatch and mutS are structurally and functionally different, so it is virtually a heterodimer.

MutS also functions as an ATPase, providing energy for the MMR pathway. After binding to the mismatch, a hemi-methylated dGATC site is located and cleaved by other MMR enzymes. This dGATC binding site is either in the 3' or 5' direction from the mismatch. The entire MMR process is a highly conserved pathway with great similarities between the human pathway and E. coli pathway. The human homolog, MutSα, acts virtually the same as the E. coli form. [5]

MutS has weak ATPase activity and may be involved in recognizing the DNA mismatch. MutS C- terminus dimerization, not tetramerization, is required for mismatch repair (Mendillo, 2007).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mutS

Mnemonic

Mutator

Synonyms

ECK2728, b2733, JW2703, fdv, ant, plm[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

61.54 minutes 

MG1655: 2855115..2857676
gbrowse_img.php?coord=2855115..2857676&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2751953..2754514
gbrowse_img.php?coord=2751953..2754514&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2855749..2858310
gbrowse_img.php?coord=2855749..2858310&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2947657..2950218
gbrowse_img.php?coord=2947657..2950218&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 2740927..2743488
gbrowse_img.php?coord=2740927..2743488&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔmutS (Keio:JW2703)

deletion

deletion

PMID:16738554

Shigen
CGSC10126[6]

mutS::Tn5KAN

PMID:12519969

ASAP

At position 2340 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20917

mutS::Tn5KAN

PMID:12519969

ASAP

At position 2340 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20918

mutS201::Tn5

CGSC:7794

mutS215::Tn10

CGSC:7797

mutS3

CGSC:11134

mutS210

CGSC:85420

mutS104::mini-Tn10

CGSC:85448

ΔmutS738::kan

PMID:16738554

CGSC:101976


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2703

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTGCAATAGAAAATTTCGA

Primer 2:CCaACCAGGCTCTTCAAGCGATA

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[6]

est. P1 cotransduction: 32% [7]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

cysI95::Tn10

Linked marker

CAG12173 = CGSC7425[6]

est. P1 cotransduction: 30% [7]
Synonyms:cysC95::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10625

EchoBASE (EcoliWiki Page)

EchoBASE:EB0620

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2733

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3294

EcoGene (EcoliWiki Page)

EcoGene:EG10625

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MUTS

CGSC (EcoliWiki Page)

CGSC:469

ASAP (EcoliWikiPage)

ASAP:ABE-0008977


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MutS

Synonyms

methyl-directed mismatch repair protein[1], B2733[2][1], Plm[2][1], Ant[2][1], Fdv[2][1], MutS[2][1]

Product description

MutS[2][3];

Component of MutHLS complex, methyl-directed mismatch repair[2][3]

Methyl-directed mismatch repair protein; dimeric/tetrameric[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0006298

mismatch repair

PMID:6987663

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0030983

mismatched DNA binding

PMID:3014530

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0006298

mismatch repair

PMID:2665076

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0005515

protein binding

PMID:15690043

IPI: Inferred from Physical Interaction

UniProtKB:P0A6N1

F

Seeded from EcoCyc [9]

complete

GO:0016887

ATPase activity

PMID:10715140

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0032300

mismatch repair complex

PMID:6987663

IGI: Inferred from Genetic Interaction

UniProtKB:P06722|UniProtKB:P23367

C

Seeded from EcoCyc 12.5 [8]

complete

GO:0000166

nucleotide binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc [9]

complete

GO:0003677

DNA binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc [9]

complete

GO:0003684

damaged DNA binding

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR007695
HAMAP:MF_00096

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0005524

ATP binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR000432
InterPro:IPR005748
InterPro:IPR007695
InterPro:IPR007696
InterPro:IPR007860
InterPro:IPR007861
SP_KW:KW-0067
HAMAP:MF_00096

F

Seeded from EcoCyc 12.5 [8]

complete

GO:0006281

DNA repair

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc [9]

complete

GO:0006298

mismatch repair

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR000432
InterPro:IPR005748
InterPro:IPR007695
InterPro:IPR007696
InterPro:IPR007860
InterPro:IPR007861
HAMAP:MF_00096

P

Seeded from EcoCyc 12.5 [8]

complete

GO:0006974

response to DNA damage stimulus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc [9]

complete

GO:0016887

ATPase activity

PMID:10715140

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [9]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006298

mismatch repair

PMID:6987663

IMP: Inferred from Mutant Phenotype

P

complete

GO:0030983

mismatched DNA binding

PMID:3014530

IDA: Inferred from Direct Assay

F

complete

GO:0006298

mismatch repair

PMID:2665076

IDA: Inferred from Direct Assay

P

complete

GO:0016887

ATPase activity

PMID:10715140

IDA: Inferred from Direct Assay

F

complete

GO:0032300

mismatch repair complex

PMID:6987663

IGI: Inferred from Genetic Interaction

UniProtKB:P06722|UniProtKB:P23367

C

P06722 (MutH) P23367 (MutL)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of MutHLS complex, methyl-directed mismatch repair

PMID:6987663

Inferred from genetic interaction

Protein

tufA

PMID:15690043

Experiment(s):EBI-886447

Protein

galF

PMID:16606699

Experiment(s):EBI-1143773

Protein

baeS

PMID:16606699

Experiment(s):EBI-1143773

Protein

envZ

PMID:16606699

Experiment(s):EBI-1143773

Protein

DnaN: beta sliding clamp subunit of DNA pol III

PMID:11459978

Protein mobility shift during native gel electrophoresis and kinase protection assay.

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL LDISLTKRGA
SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA TSKGPVERKV VRIVTPGTIS
DEALLQERQD NLLAAIWQDS KGFGYATLDI SSGRFRLSEP ADRETMAAEL QRTNPAELLY
AEDFAEMSLI EGRRGLRRRP LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL
LQYAKDTQRT TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT
PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER ILARLALRTA
RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF AELRDLLERA IIDTPPVLVR
DGGVIASGYN EELDEWRALA DGATDYLERL EVRERERTGL DTLKVGFNAV HGYYIQISRG
QSHLAPINYM RRQTLKNAER YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE
ALQQSASALA ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP
LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI DRIFTRVGAA
DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS TYDGLSLAWA CAENLANKIK
ALTLFATHYF ELTQLPEKME GVANVHLDAL EHGDTIAFMH SVQDGAASKS YGLAVAALAG
VPKEVIKRAR QKLRELESIS PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR
QALEWIYRLK SLV
Length

853

Mol. Wt

95.249 kDa

pI

5.4 (calculated)

Extinction coefficient

73,230 - 73,980 (calc based on 27 Y, 6 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10625-MONOMER

UniProt (EcoliWiki Page)

UniProt:P23909

PFAM (EcoliWiki Page)

PFAM:PF01624

RefSeq (EcoliWiki Page)

RefSeq:NP_417213


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mutS

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=2855095..2855135&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2733 (EcoliWiki Page)

NCBI GEO profiles for mutS

microarray

GenExpDB:b2733 (EcoliWiki Page)

Summary of data for mutS from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (2854755..2855144) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ12; Well:E1[10]

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Notes

Accessions Related to mutS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10625

EchoBASE (EcoliWiki Page)

EchoBASE:EB0620

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2733

EcoGene (EcoliWiki Page)

EcoGene:EG10625


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000014707 (score: 1.000; bootstrap: 62%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000013289 (score: 1.000; bootstrap: 85%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT3G24495 (score: 1.000; bootstrap: 62%)
  • AT4G02070 (score: 0.103)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000001867 (score: 1.000; bootstrap: 89%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00035331 (score: 1.000; bootstrap: 67%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00003422 (score: 1.000; bootstrap: 51%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000003882 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000008912 (score: 1.000; bootstrap: 73%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-020905-3 (score: 1.000; bootstrap: 68%)
  • ZDB-CDNA-040425-552 (score: 1.000; bootstrap: 69%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0232360 (score: 1.000; bootstrap: 98%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036486 (score: 1.000; bootstrap: 61%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA20021-PA (score: 1.000; bootstrap: 64%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000014557 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000234420 (score: 1.000; bootstrap: 55%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000024039 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000001344 (score: 1.000; bootstrap: 62%)

From Inparanoid:20070104

Mus musculus

  • MGI:1343961 (score: 1.000; bootstrap: 50%)

From Inparanoid:20070104

Oryza gramene

  • Q5VR41 (score: 1.000; bootstrap: 80%)
  • Q8RVT1 (score: 0.676)
  • Q9XGD0 (score: 0.608)
  • Q9T0N6 (score: 0.510)
  • Q69MX6 (score: 0.075)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000020434 (score: 1.000; bootstrap: 78%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000018449 (score: 1.000; bootstrap: 56%)
  • ENSRNOP00000021923 (score: 1.000; bootstrap: 54%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YHR120W (score: 1.000; bootstrap: 78%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC2856c (score: 1.000; bootstrap: 50%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000166199 (score: 1.000; bootstrap: 67%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00028603001 (score: 1.000; bootstrap: 84%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000042811 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

MUTS

From SHIGELLACYC

E. coli O157

MUTS

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF01624


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Li GM (2008) Mechanisms and functions of DNA mismatch repair. Cell Res 18: 85-98 PubMed EcoliWiki page
  6. ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 8.7 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  10. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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