nadE:Gene Product(s)

Standard name	NadE
Synonyms	NAD synthetase, NH3/glutamine-dependent^[1], B1740^[2]^[1], Efg^[2]^[1], NtrL^[2]^[1], NadE^[2]^[1]
Product description	NadE^[2]^[3]; Component of NAD-SYNTH-CPLX^[2] NAD synthase, ammonia dependent^[4]
EC number (for enzymes)	6.3.5.1^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P00350	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P00509	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P00935	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P04425	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P05804	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P06715	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P08204	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P09148	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A7G2	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A8M0	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A8Y8	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9A9	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9D4	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9P4	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0A9W3	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0AC41	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0ADU2	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0ADV9	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0AEA8	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0AGB0	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0AGF2	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P21363	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P24215	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P25516	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P28722	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P28903	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P36553	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P36938	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P64461	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P68206	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P68661	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P69816	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P69902	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P75823	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P75937	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P76364	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P76558	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0005515	protein binding	PMID:16606699^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P77148	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0008795	NAD+ synthase activity	PMID:10544053^[7]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0034355	NAD salvage	PMID:10544053^[7]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc ^[8]	complete
	GO:0034628	de novo NAD biosynthetic process from aspartate	PMID:10544053^[7]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc ^[8]	complete
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc ^[8]	complete
	GO:0003952	NAD+ synthase (glutamine-hydrolyzing) activity	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003694	F	Seeded from EcoCyc ^[8]	complete
	GO:0005524	ATP binding	GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR003694 SP_KW:KW-0067 HAMAP:MF_00193	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0008795	NAD+ synthase activity	GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:6.3.1.5	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0009435	NAD biosynthetic process	GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR003694 HAMAP:MF_00193	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0016874	ligase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0436	F	Seeded from EcoCyc ^[8]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of NAD-SYNTH-CPLX	could be indirect
Protein	arnA		PMID:15690043^[9]	Experiment(s):EBI-887727
Protein	ygfH		PMID:15690043^[9]	Experiment(s):EBI-887727
Protein	yhcA		PMID:16606699^[5]	Experiment(s):EBI-1140662
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Notes

Localization

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Compartment	Description	Evidence	Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG QDSTLAGKLC QMAINELRLE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA GMTSGVVVGT DHAAEAITGF FTKYGDGGTD INPLYRLNKR QGKQLLAALA CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG KNVPQQVART IENWYLKTEH KRRPPITVFD DFWKK
Length	275
Mol. Wt	30.637 kDa
pI	5.3 (calculated)
Extinction coefficient	25,900 - 26,275 (calc based on 10 Y, 2 W, and 3 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
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Structure
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Structures

Representative Escherichia coli structure:
1WXE|A
1-275 of 275 residues (E-value: 2e-145) (Percent Identity: 100.00)
View the PDB Table for nadE

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:NAD-SYNTH-MONOMER
UniProt (EcoliWiki Page)	UniProt:P18843
PFAM (EcoliWiki Page)	PFAM:PF02540
RefSeq (EcoliWiki Page)	RefSeq:NP_416254
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.00 ^5.01 ^5.02 ^5.03 ^5.04 ^5.05 ^5.06 ^5.07 ^5.08 ^5.09 ^5.10 ^5.11 ^5.12 ^5.13 ^5.14 ^5.15 ^5.16 ^5.17 ^5.18 ^5.19 ^5.20 ^5.21 ^5.22 ^5.23 ^5.24 ^5.25 ^5.26 ^5.27 ^5.28 ^5.29 ^5.30 ^5.31 ^5.32 ^5.33 ^5.34 ^5.35 ^5.36 ^5.37 ^5.38 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ ^6.00 ^6.01 ^6.02 ^6.03 ^6.04 ^6.05 ^6.06 ^6.07 ^6.08 ^6.09 ^6.10 ^6.11 ^6.12 ^6.13 ^6.14 ^6.15 ^6.16 ^6.17 ^6.18 ^6.19 ^6.20 ^6.21 ^6.22 ^6.23 ^6.24 ^6.25 ^6.26 ^6.27 ^6.28 ^6.29 ^6.30 ^6.31 ^6.32 ^6.33 ^6.34 ^6.35 ^6.36 ^6.37 ^6.38 ^6.39 ^6.40 ^6.41 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^7.0 ^7.1 ^7.2 Ozment C et al. (1999) Structural study of Escherichia coli NAD synthetase: overexpression, purification, crystallization, and preliminary crystallographic analysis. J Struct Biol 127: 279-82 PubMed EcoliWiki page
↑ ^8.0 ^8.1 ^8.2 ^8.3 ^8.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^9.0 ^9.1 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page

nadE:Gene Product(s)

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Nomenclature

Notes

Function

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Localization

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Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

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Accessions in Other Databases

Notes

Links

References

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