napA:Gene - EcoliWiki
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napA:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

napA

Mnemonic
Synonyms

ECK2198, b2206, JW2194, yojC, yojD, yojE[1]

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Notes

Location(s) and DNA Sequence

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StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

49.54 minutes 

MG1655: 2300775..2298289
gbrowse_img.php?coord=2297289..2301775&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2306087..2303601
gbrowse_img.php?coord=2302601..2307087&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔnapA (Keio:JW2194)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9774[3]

napA722::Tn10

Insertion at 2,299,612 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12098 = CGSC7400[3]

Synonyms: zeg-722, zei-722::Tn10

napA722::Tn10

PMID:2540407[5] PMID:9829956[6]

CGSC:40001

napA3130::Tn10kan

PMID:2540407[5] PMID:9829956[6]

CGSC:40004

ΔnapA759::kan

PMID:16738554[2]

CGSC:102010


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Notes

Genetic Resources

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ResourceResource TypeSourceNotes/Reference

JW2194

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAACTCAGTCGTCGTAGCTT

Primer 2:CCaACCTTCTCCAGTTTGACCGC

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[3]

est. P1 cotransduction: % [4]

Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

atoS298::Tn10

Linked marker

CAG12177 gyrA+ = CGSC7525[3]

est. P1 cotransduction: 45% [4]

Synonyms:zeh-298::Tn10, zej-298::Tn10


The original CAG12177 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

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Notes

Accessions in Other Databases

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DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12067

EchoBASE (EcoliWiki Page)

EchoBASE:EB1994

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2206

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2745

EcoGene (EcoliWiki Page)

EcoGene:EG12067

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:NAPA

CGSC (EcoliWiki Page)

CGSC:36550

ASAP (EcoliWikiPage)

ASAP:ABE-0007289


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Notes

Links

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NameURLComments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. 5.0 5.1 Singer M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev 53: 1-24 PubMed EcoliWiki page
  6. 6.0 6.1 Nichols BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J Bacteriol 180: 6408-11 PubMed EcoliWiki page
  7. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page

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