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narH:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

narH

Mnemonic

Nitrate reductase, nitrate regulation

Synonyms

ECK1219, b1225, JW1216, chlC[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

27.65 minutes 

MG1655: 1282827..1284365
gbrowse_img.php?coord=1282827..1284365&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1283754..1285292
gbrowse_img.php?coord=1283754..1285292&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1285181..1286719
gbrowse_img.php?coord=1285181..1286719&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1323068..1324606
gbrowse_img.php?coord=1323068..1324606&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1171548..1173086
gbrowse_img.php?coord=1171548..1173086&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔnarH (Keio:JW1216)

deletion

deletion

PMID:16738554[2]

Shigen


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW1216

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAAAATTCGTTCACAAGTCGG

Primer 2:CCTGGATGCGGCTCCGTTTTGCT

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[4]

est. P1 cotransduction: 11% [5]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[4]

est. P1 cotransduction: 46% [5]
Synonyms:zch-506::Tn10, zci-506::Tn10

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10639

EchoBASE (EcoliWiki Page)

EchoBASE:EB0633

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1225

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1686

EcoGene (EcoliWiki Page)

EcoGene:EG10639

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:NARH

CGSC (EcoliWiki Page)

CGSC:18118

ASAP (EcoliWikiPage)

ASAP:ABE-0004121


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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