narH:Gene Product(s)

Standard name	NarH
Synonyms	nitrate reductase 1, beta (Fe-S) subunit^[1], B1225^[2]^[1], ChlC^[2]^[1], NarH^[2]^[1]
Product description	nitrate reductase A, β subunit^[2]^[3]; Component of NarGH catalytic dimer of nitrate reductase A^[3]; nitrate reductase A^[2]^[3] Nitrate reductase I (NRA), beta subunit^[4]
EC number (for enzymes)	1.7.99.4^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:15690043^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P19319	F	Seeded from EcoCyc ^[6]	complete
	GO:0009055	electron carrier activity	PMID:10504245^[7]	IPI: Inferred from Physical Interaction		F	Seeded from EcoCyc ^[6]	complete
	GO:0009061	anaerobic respiration	PMID:7050087^[8]	IEP: Inferred from Expression Pattern		P	Seeded from EcoCyc ^[6]	complete
	GO:0017004	cytochrome complex assembly	PMID:3053688^[9]	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc ^[6]	complete
	GO:0031224	intrinsic to membrane	PMID:5996^[10]	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc ^[6]	complete
	GO:0051538	3 iron, 4 sulfur cluster binding	PMID:8664273^[11]	IPI: Inferred from Physical Interaction		F	Seeded from EcoCyc 12.5 ^[12]	complete
	GO:0051539	4 iron, 4 sulfur cluster binding	PMID:14725769^[13]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc 12.5 ^[12]	complete
	GO:0005506	iron ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc ^[6]	complete
	GO:0006810	transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc ^[6]	complete
	GO:0008940	nitrate reductase activity	GO_REF:0000002 GO_REF:0000003	IEA: Inferred from Electronic Annotation	InterPro:IPR006547 EC:1.7.99.4	F	Seeded from EcoCyc ^[6]	complete
	GO:0009325	nitrate reductase complex	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006547	C	Seeded from EcoCyc ^[6]	complete
	GO:0042128	nitrate assimilation	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0534	P	Seeded from EcoCyc ^[6]	complete
	GO:0051538	3 iron, 4 sulfur cluster binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0003	F	Seeded from EcoCyc 12.5 ^[12]	complete
	GO:0051539	4 iron, 4 sulfur cluster binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0004	F	Seeded from EcoCyc 12.5 ^[12]	complete
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	required fields missing
	GO:0005886	plasma membrane	GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003 SP_SL:SL-0039	C	Seeded from EcoCyc ^[6]	complete
	GO:0016020	membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc ^[6]	complete
	GO:0016491	oxidoreductase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc ^[6]	complete
	GO:0022900	electron transport chain	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0249	P	Seeded from EcoCyc ^[6]	complete
	GO:0042126	nitrate metabolic process	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006547	P	Seeded from EcoCyc ^[6]	complete
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc ^[6]	complete
	GO:0051536	iron-sulfur cluster binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0411	F	Seeded from EcoCyc ^[6]	complete
	GO:0055114	oxidation reduction	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc ^[6]	complete
	GO:0009054	electron acceptor activity				F	Seeded from EcoCyc 11.1^[3].	required fields missing
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of NarGH catalytic dimer of nitrate reductase A	could be indirect
Protein	ugpB		PMID:16606699^[14]	Experiment(s):EBI-1139225
Protein	nadE		PMID:16606699^[14]	Experiment(s):EBI-1139225
Protein	narG		PMID:15690043^[5]	Experiment(s):EBI-880342, EBI-885754
Protein	tufA		PMID:15690043^[5]	Experiment(s):EBI-880342
Protein	fadH		PMID:15690043^[5]	Experiment(s):EBI-885754
Protein	Subunits of nitrate reductase A	could be indirect
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Notes

Localization

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Compartment	Description	Evidence	Source	Notes
Cytoplasm		PMID:3053688^[9], PMID:2233673^[15], PMID:2674654^[16]	EchoLocation:narH
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TSREGVEYAW FNNVETKPGQ GFPTDWENQE KYKGGWIRKI NGKLQPRMGN RAMLLGKIFA NPHLPGIDDY YEPFDFDYQN LHTAPEGSKS QPIARPRSLI TGERMAKIEK GPNWEDDLGG EFDKLAKDKN FDNIQKAMYS QFENTFMMYL PRLCEHCLNP ACVATCPSGA IYKREEDGIV LIDQDKCRGW RMCITGCPYK KIYFNWKSGK SEKCIFCYPR IEAGQPTVCS ETCVGRIRYL GVLLYDADAI ERAASTENEK DLYQRQLDVF LDPNDPKVIE QAIKDGIPLS VIEAAQQSPV YKMAMEWKLA LPLHPEYRTL PMVWYVPPLS PIQSAADAGE LGSNGILPDV ESLRIPVQYL ANLLTAGDTK PVLRALKRML AMRHYKRAET VDGKVDTRAL EEVGLTEAQA QEMYRYLAIA NYEDRFVVPS SHRELAREAF PEKNGCGFTF GDGCHGSDTK FNLFNSRRID AIDVTSKTEP HP
Length	512
Mol. Wt	58.067 kDa
pI	6.8 (calculated)
Extinction coefficient	82,280 - 84,405 (calc based on 22 Y, 9 W, and 17 C residues)
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Domains/Motifs/Modification Sites

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Type	Residues	Description	Notes	References
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Structure
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Structures

Representative Escherichia coli structure:
1Q16|B
1-512 of 512 residues (E-value: 2e-289) (Percent Identity: 100.00)
View the PDB Table for narH

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:NARH-MONOMER
UniProt (EcoliWiki Page)	UniProt:P11349
PFAM (EcoliWiki Page)	PFAM:PF00037
RefSeq (EcoliWiki Page)	RefSeq:NP_415743
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 Butland G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-7 PubMed EcoliWiki page
↑ ^6.00 ^6.01 ^6.02 ^6.03 ^6.04 ^6.05 ^6.06 ^6.07 ^6.08 ^6.09 ^6.10 ^6.11 ^6.12 ^6.13 ^6.14 ^6.15 ^6.16 ^6.17 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ Rothery RA et al. (1999) The hemes of Escherichia coli nitrate reductase A (NarGHI): potentiometric effects of inhibitor binding to narI. Biochemistry 38: 12747-57 PubMed EcoliWiki page
↑ Stewart V (1982) Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12. J Bacteriol 151: 1320-5 PubMed EcoliWiki page
↑ ^9.0 ^9.1 Sodergren EJ et al. (1988) Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli. J Biol Chem 263: 16156-62 PubMed EcoliWiki page
↑ Garland PB et al. (1975) Proton translocation and the respiratory nitrate reductase of Escherichia coli. Biochem J 152: 547-59 PubMed EcoliWiki page
↑ Guigliarelli B et al. (1996) Complete coordination of the four Fe-S centers of the beta subunit from Escherichia coli nitrate reductase. Physiological, biochemical, and EPR characterization of site-directed mutants lacking the highest or lowest potential [4Fe-4S] clusters. Biochemistry 35: 4828-36 PubMed EcoliWiki page
↑ ^12.0 ^12.1 ^12.2 ^12.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Jormakka M et al. (2004) Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes. Structure 12: 95-104 PubMed EcoliWiki page
↑ ^14.0 ^14.1 Arifuzzaman M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res 16: 686-91 PubMed EcoliWiki page
↑ Blasco F et al. (1990) Nitrate reductases of Escherichia coli: sequence of the second nitrate reductase and comparison with that encoded by the narGHJI operon. Mol Gen Genet 222: 104-11 PubMed EcoliWiki page
↑ Blasco F et al. (1989) Nitrate reductase of Escherichia coli: completion of the nucleotide sequence of the nar operon and reassessment of the role of the alpha and beta subunits in iron binding and electron transfer. Mol Gen Genet 218: 249-56 PubMed EcoliWiki page

narH:Gene Product(s)

From EcoliWiki

Nomenclature

Notes

Function

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Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

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Accessions in Other Databases

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Links

References

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