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nhaA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NhaA

Synonyms

sodium-proton antiporter[1], B0019[2][1], Hyc[2][1], Ant[2][1], AntA[2][1], NhaA[2][1]

Product description

sodium/proton NhaA transporter[2][3]

Na+/H+ antiporter 1, strongly pH-dependent; helps regulate intracellular pH and extrude lithium; nhaA_P1 activated by NhaR, repressed by H-NS and stimulated by Na(+)[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0006814

sodium ion transport

PMID:1645730[5]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0006883

cellular sodium ion homeostasis

PMID:1332943[7]

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc [8]

complete

GO:0015491

cation:cation antiporter activity

PMID:1645730[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [8]

complete

GO:0015992

proton transport

PMID:1645730[5]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc [8]

complete

GO:0006814

sodium ion transport

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004670
SP_KW:KW-0739

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0006885

regulation of pH

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004670

P

Seeded from EcoCyc [8]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004670
SP_KW:KW-0812

C

Seeded from EcoCyc [8]

complete

GO:0031402

sodium ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0915

F

Seeded from EcoCyc [8]

complete

GO:0015292

uniporter activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000020
GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

HAMAP:MF_01844
SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [8]

complete

GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [8]

complete

GO:0006811

ion transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0406

P

Seeded from EcoCyc [8]

complete

GO:0015081

sodium ion transmembrane transporter activity

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01844

F

Seeded from EcoCyc [8]

complete

GO:0015297

antiporter activity

GO_REF:0000020
GO_REF:0000004

IEA: Inferred from Electronic Annotation

HAMAP:MF_01844
SP_KW:KW-0050

F

Seeded from EcoCyc [8]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

plasma membrane

C-terminus localized in the cytoplasm with 11 predicted transmembrane domains

Daley et al. (2005) [9]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MKHLHRFFSS DASGGIILII AAILAMIMAN SGATSGWYHD FLETPVQLRV GSLEINKNML
LWINDALMAV FFLLVGLEVK RELMQGSLAS LRQAAFPVIA AIGGMIVPAL LYLAFNYADP
ITREGWAIPA ATDIAFALGV LALLGSRVPL ALKIFLMALA IIDDLGAIII IALFYTNDLS
MASLGVAAVA IAVLAVLNLC GARRTGVYIL VGVVLWTAVL KSGVHATLAG VIVGFFIPLK
EKHGRSPAKR LEHVLHPWVA YLILPLFAFA NAGVSLQGVT LDGLTSILPL GIIAGLLIGK
PLGISLFCWL ALRLKLAHLP EGTTYQQIMV VGILCGIGFT MSIFIASLAF GSVDPELINW
AKLGILVGSI SSAVIGYSWL RVRLRPSV
Length

388

Mol. Wt

41.357 kDa

pI

9.6 (calculated)

Extinction coefficient

55,920 - 56,295 (calc based on 8 Y, 8 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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tmhmm.php?gene=nhaA&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:NHAA-MONOMER

UniProt (EcoliWiki Page)

UniProt:P13738

PFAM (EcoliWiki Page)

PFAM:PF06965

RefSeq (EcoliWiki Page)

RefSeq:NP_414560


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Taglicht D et al. (1991) Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. J Biol Chem 266: 11289-94 PubMed EcoliWiki page
  6. ↑ 6.0 6.1 6.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ Ohyama T et al. (1992) Escherichia coli is able to grow with negligible sodium ion extrusion activity at alkaline pH. J Bacteriol 174: 7743-9 PubMed EcoliWiki page
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  9. ↑ Daley DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308: 1321-3 PubMed EcoliWiki page

Categories

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