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nlpA:Gene

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nlpA

Mnemonic

New lipoprotein

Synonyms

ECK3651, b3661, JW3635[1]

Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

82.7 minutes 

MG1655: 3838016..3837198
gbrowse_img.php?coord=3837198..3838016&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3799925..3799107
gbrowse_img.php?coord=3799107..3799925&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3800422..3801240
gbrowse_img.php?coord=3800422..3801240&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3935600..3934782
gbrowse_img.php?coord=3934782..3935600&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 3725531..3726349
gbrowse_img.php?coord=3725531..3726349&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription

Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔnlpA (Keio:JW3635)

deletion

deletion

PMID:16738554[2]

Shigen

nlpA::kan

Other

nonessential

PMID:2841301[3]

nlpA3085::Tn10

PMID:9829956[4] PMID:2540407[5]

CGSC:59412


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW3635

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAACTGACAACACATCATCT

Primer 2:CCCCAGCCAGGCACCGCGCCACC

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[7]

est. P1 cotransduction: 72% [8]

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[7]

est. P1 cotransduction: 22% [8]

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10657

EchoBASE (EcoliWiki Page)

EchoBASE:EB0651

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3661

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4583

EcoGene (EcoliWiki Page)

EcoGene:EG10657

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:NLPA

CGSC (EcoliWiki Page)

CGSC:35704

ASAP (EcoliWikiPage)

ASAP:ABE-0011964


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2: 2006.0008 PubMed EcoliWiki page
  3. Yamaguchi K & Inouye M (1988) Lipoprotein 28, an inner membrane protein of Escherichia coli encoded by nlpA, is not essential for growth. J Bacteriol 170: 3747-9 PubMed EcoliWiki page
  4. Nichols BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J Bacteriol 180: 6408-11 PubMed EcoliWiki page
  5. Singer M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev 53: 1-24 PubMed EcoliWiki page
  6. Kitagawa M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (A Complete Set of E. coli K-12 ORF Archive): Unique Resources for Biological Research. DNA Res 12: 291-9 PubMed EcoliWiki page
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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