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nuoB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NuoB

Synonyms

NADH:ubiquinone oxidoreductase, chain B[1], B2287[2][1], NuoB[2][1]

Product description

NuoB[2][3];

Component of connecting fragment of NADH dehydrogenase I[3]; NADH dehydrogenase I[2][3]

NADH:ubiquinone oxidoreductase subunit B, complex I; NADH dehydrogenase I[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus
GO:0005886

plasma membrane

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0008137

NADH dehydrogenase (ubiquinone) activity

PMID:9495756[7]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0009060

aerobic respiration

PMID:9495756[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [8]

complete

GO:0009061

anaerobic respiration

PMID:9063459[9]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [8]

complete

GO:0015453

oxidoreduction-driven active transmembrane transporter activity

PMID:3122832[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [8]

complete

GO:0015990

electron transport coupled proton transport

PMID:16807239[11]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [8]

complete

GO:0030964

NADH dehydrogenase complex

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [8]

complete

GO:0045272

plasma membrane respiratory chain complex I

PMID:11997136[12]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [8]

complete

GO:0051539

4 iron, 4 sulfur cluster binding

PMID:12975362[13]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0005506

iron ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0006120

mitochondrial electron transport, NADH to ubiquinone

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006137
InterPro:IPR006138

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0008137

NADH dehydrogenase (ubiquinone) activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006137
InterPro:IPR006138

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0046872

metal ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc [8]

complete

GO:0048038

quinone binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0874

F

Seeded from EcoCyc [8]

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR014406
SP_KW:KW-0004

F

Seeded from EcoCyc 12.5 [6]

complete

NOT

GO:0005737

cytoplasm

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

See discussion page for explanation of the 'NOT' qualifier.

complete

GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [8]

complete

GO:0016020

membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc [8]

complete

GO:0016491

oxidoreductase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc [8]

complete

GO:0016651

oxidoreductase activity, acting on NADH or NADPH

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014406

F

Seeded from EcoCyc [8]

complete

GO:0019684

photosynthesis, light reaction

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01356

P

Seeded from EcoCyc [8]

complete

GO:0050136

NADH dehydrogenase (quinone) activity

GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.6.99.5

F

Seeded from EcoCyc [8]

complete

GO:0051536

iron-sulfur cluster binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0411

F

Seeded from EcoCyc [8]

complete

GO:0055114

oxidation reduction

GO_REF:0000020
GO_REF:0000004

IEA: Inferred from Electronic Annotation

HAMAP:MF_01356
SP_KW:KW-0560

P

Seeded from EcoCyc [8]

complete

GO:0009061

anaerobic respiration

PMID:9063459[9]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 11.1[3].

Growth with fumarate as the terminal electron acceptor requires NADH dehydrogenase I.

complete

GO:0009053

electron donor activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0015453

oxidoreduction-driven active transmembrane transporter activity

PMID:3122832[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 11.1[3].

NADH dehydrogenase I in membrane vesicles can generate a H+ electrochemical gradient in the presence of deamino-NADH and ubiquinone-1.

complete

GO:0030964

NADH dehydrogenase complex

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

The E. coli NADH dehydrogenase complex was purified and the subunits separated by SDS-PAGE. The N-termini of these 6 polypeptides were sequenced after deblocking with methanolic HCl.

complete

GO:0005886

plasma membrane

PMID:7607227[5]

IDA: Inferred from Direct Assay

C

The NADH dehydrogenase I complex fractionates with cytoplasmic membranes.

complete

Contributes to

GO:0003954

NADH dehydrogenase activity

PMID:3122832[10]

IDA: Inferred from Direct Assay

F

Purified NADH dehydrogenase I in membrane vesicles can oxidize NADH.

complete

GO:0051539

4 iron, 4 sulfur cluster binding

PMID:12975362[13]

IMP: Inferred from Mutant Phenotype

F

Mutation of cysteine residues in nuoB results in loss of the EPR spectral signal characteristic of the N2 [4Fe-4S] cluster in NADH dehydrogenase I.

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

Subunits of connecting fragment of NADH dehydrogenase I

could be indirect

Protein

Subunits of NADH dehydrogenase I

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

Cytoplasm

PMID:7607227[5]

EchoLocation:nuoB


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR KNSIWPYNFG
LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG TCFTKMAPVI QRLYDQMLEP
KWVISMGACA NSGGMYDIYS VVQGVDKFIP VDVYIPGCPP RPEAYMQALM LLQESIGKER
RPLSWVVGDQ GVYRANMQSE RERKRGERIA VTNLRTPDEI
Length

220

Mol. Wt

25.055 kDa

pI

5.6 (calculated)

Extinction coefficient

36,900 - 37,525 (calc based on 10 Y, 4 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:NUOB-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AFC7

UniProt (EcoliWiki Page)

UniProt:P0AFC7

PFAM (EcoliWiki Page)

PFAM:PF01058

RefSeq (EcoliWiki Page)

RefSeq:NP_416790


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Leif H et al. (1995) Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli. Eur J Biochem 230: 538-48 PubMed EcoliWiki page
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ 7.0 7.1 Falk-Krzesinski HJ & Wolfe AJ (1998) Genetic analysis of the nuo locus, which encodes the proton-translocating NADH dehydrogenase in Escherichia coli. J Bacteriol 180: 1174-84 PubMed EcoliWiki page
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  9. ↑ 9.0 9.1 Tran QH et al. (1997) Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications. Eur J Biochem 244: 155-60 PubMed EcoliWiki page
  10. ↑ 10.0 10.1 10.2 Matsushita K et al. (1987) NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain. Biochemistry 26: 7732-7 PubMed EcoliWiki page
  11. ↑ Flemming D et al. (2006) Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I). J Biol Chem 281: 24781-9 PubMed EcoliWiki page
  12. ↑ David P et al. (2002) Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues. Biochim Biophys Acta 1553: 268-78 PubMed EcoliWiki page
  13. ↑ 13.0 13.1 Flemming D et al. (2003) Iron-sulfur cluster N2 of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I) is located on subunit NuoB. J Biol Chem 278: 47602-9 PubMed EcoliWiki page

Categories

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