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nupG:On One Page

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Standard Name

nupG

Gene Synonym(s)

ECK2959, b2964, JW2932[1]

Product Desc.

NupG nucleoside MFS transporter[2][3]

Nucleoside transporter[4]

Product Synonyms(s)

nucleoside transporter[1], B2964[2][1], NupG[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yggX-mltC-nupG[2], mutY-yggX-mltC-nupG[2], nupG[2], OP00172

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(nupG)    Textpresso(nupG)

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Notes

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NupG, XapB and YegT are paralogs. Major Facilitator Superfamily (MFS). CytR regulon. DeoR regulon.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nupG

Mnemonic

Nucleoside permease

Synonyms

ECK2959, b2964, JW2932[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

66.9 minutes 

MG1655: 3103736..3104992
gbrowse_img.php?coord=3103736..3104992&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2991375..2992631
gbrowse_img.php?coord=2991375..2992631&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3104370..3105626
gbrowse_img.php?coord=3104370..3105626&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

BW2952

NC_012759: 2990884..2992140
gbrowse_img.php?coord=2990884..2992140&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

ΔnupG (Keio:JW2932)

deletion

deletion

PMID:16738554

Shigen
CGSC10267[5]

nupG::Tn5KAN

PMID:12519969

ASAP

At position 723 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20994

nupG::Tn5KAN

PMID:12519969

ASAP

At position 723 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20995

nupG511::Tn10

Insertion at 3,104,399 bp in MG1655 (NC_000913)

adapted from Nichols et al.[6]

CAG18472 = CGSC7433[5]

Synonyms:

nupG511::Tn10

CGSC:8684

nupG3157::Tn10kan

PMID:2540407

CGSC:40115

ΔnupG739::kan

PMID:16738554

CGSC:102166


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Notes

Mutants containing the nupG system were obtained by mating and recombination in a transport negative strain, SΦ587. [7] The nupG system determines high-affinity nucleoside transport across the cell membrane. nupG+ results in nucleoside transport and these imported nucleosides serve as antecedents of DNA and RNA. nupG- is a mutant and does not allow nucleoside transport across the membrane. [8]

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

JW2932

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAATCTTAAGCTGCAGCTGAA

Primer 2:CCGTGGCTAACCGTCTGTGTGCC

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[5]

est. P1 cotransduction: 52% [6]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

metC162::Tn10

Linked marker

CAG18475 = CGSC7435[5]

est. P1 cotransduction: 12% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10664

EchoBASE (EcoliWiki Page)

EchoBASE:EB0658

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2964

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3552

EcoGene (EcoliWiki Page)

EcoGene:EG10664

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:NUPG

CGSC (EcoliWiki Page)

CGSC:442

ASAP (EcoliWikiPage)

ASAP:ABE-0009728


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NupG

Synonyms

nucleoside transporter[1], B2964[2][1], NupG[2][1]

Product description

NupG nucleoside MFS transporter[2][3]

Nucleoside transporter[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0005215

transporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007114

F

Seeded from EcoCyc [9]

complete

GO:0005337

nucleoside transmembrane transporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004740

F

Seeded from EcoCyc [9]

complete

GO:0006810

transport

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR007114
SP_KW:KW-0813

P

Seeded from EcoCyc [9]

complete

GO:0015858

nucleoside transport

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004740

P

Seeded from EcoCyc [9]

complete

GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [10]

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004740
InterPro:IPR007114
SP_KW:KW-0812

C

Seeded from EcoCyc [9]

complete

GO:0015292

uniporter activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [9]

complete

GO:0015949

nucleobase, nucleoside and nucleotide interconversion

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [11]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MCFFKHSSAS RCEEINMNLK LQLKILSFLQ FCLWGSWLTT LGSYMFVTLK FDGASIGAVY
SSLGIAAVFM PALLGIVADK WLSAKWVYAI CHTIGAITLF MAAQVTTPEA MFLVILINSF
AYMPTLGLIN TISYYRLQNA GMDIVTDFPP IRIWGTIGFI MAMWVVSLSG FELSHMQLYI
GAALSAILVL FTLTLPHIPV AKQQANQSWT TLLGLDAFAL FKNKRMAIFF IFSMLLGAEL
QITNMFGNTF LHSFDKDPMF ASSFIVQHAS IIMSISQISE TLFILTIPFF LSRYGIKNVM
MISIVAWILR FALFAYGDPT PFGTVLLVLS MIVYGCAFDF FNISGSVFVE KEVSPAIRAS
AQGMFLMMTN GFGCILGGIV SGKVVEMYTQ NGITDWQTVW LIFAGYSVVL AFAFMAMFKY
KHVRVPTGTQ TVSH
Length

434

Mol. Wt

48.262 kDa

pI

8.6 (calculated)

Extinction coefficient

74,370 - 75,120 (calc based on 13 Y, 10 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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tmhmm.php?gene=nupG&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:NUPG-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AFF4

PFAM (EcoliWiki Page)

PFAM:PF03825

RefSeq (EcoliWiki Page)

RefSeq:NP_417439


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yggX-mltC-nupG

mutY-yggX-mltC-nupG

nupG

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=3103716..3103756&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b2964 (EcoliWiki Page)

NCBI GEO profiles for nupG

microarray

GenExpDB:b2964 (EcoliWiki Page)

Summary of data for nupG from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes

GFP Fusion

Intergenic region (3103439..3103701) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ16; Well:E8[12]

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Notes

Accessions Related to nupG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10664

EchoBASE (EcoliWiki Page)

EchoBASE:EB0658

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2964

EcoGene (EcoliWiki Page)

EcoGene:EG10664


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Anopheles gambiae

  • ENSANGP00000010355 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036191 (score: 1.000; bootstrap: 100%)
  • FBgn0034093 (score: 0.149)

From Inparanoid:20070104

Shigella flexneri

NUPG

From SHIGELLACYC

E. coli O157

NUPG

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF03825


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Munch-Petersen A & Mygind B (1976) Nucleoside transport systems in Escherichia coli K12: specificity and regulation. J Cell Physiol 89: 551-9 PubMed EcoliWiki page
  8. ↑ Westh Hansen SE et al. (1987) Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system. Eur J Biochem 168: 385-91 PubMed EcoliWiki page
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  10. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  11. ↑ Daley DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308: 1321-3 PubMed EcoliWiki page
  12. ↑ Zaslaver A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat Methods 3: 623-8 PubMed EcoliWiki page

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