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Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

ogt

Gene Synonym(s)

ECK1331, b1335, JW1329[1]

Product Desc.

Ogt[2][3]

O6-methylguanine-DNA methyltransferase, constitutive[4]

Product Synonyms(s)

O-6-alkylguanine-DNA:cysteine-protein methyltransferase[1], O-6-alkylguanine-DNA/Cysteine-protein-methyltransferase[2][1], B1335[2][1], Ogt[2][1]

Function from GO
Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ogt[2]

Regulation/Activity
Quick Links

DNA display    Protein display    Pubmed(ogt)    Textpresso(ogt)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ogt

Mnemonic

O-methylguanine transferase

Synonyms

ECK1331, b1335, JW1329[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

StrainMap locationGenome coordinatesGenome browsersSequence links

MG1655

30.13 minutes 

MG1655: 1398260..1397745
gbrowse_img.php?coord=1397745..1398260&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1397406..1396891
gbrowse_img.php?coord=1396891..1397406&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1401950..1401435
gbrowse_img.php?coord=1401435..1401950&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1487656..1487141
gbrowse_img.php?coord=1487141..1487656&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

BW2952

NC_012759: 1289804..1290319
gbrowse_img.php?coord=1289804..1290319&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature typeLocationDescription
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

AlleleNt change(s)AA change(s)Phenotype: TypePhenotype: DescriptionReferenceAvailabilityComments

Δogt (Keio:JW1329)

deletion

deletion

PMID:16738554

Shigen
CGSC9188[5]

ogtC102G

C102G

Little effect on the activity

seeded from UniProt:P0AFH0

ogtC139G

C139G

Eliminates the activity

seeded from UniProt:P0AFH0

Δogt-772::kan

PMID:16738554

CGSC:102172


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

ResourceResource TypeSourceNotes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[5]

est. P1 cotransduction: 32% [6]
Synonyms:zci-233::Tn10

recT3061::Tn10

Linked marker

CAG12081 = CGSC7375[5]

est. P1 cotransduction: 64% [6]
Synonyms:zcj-3061::Tn10, zda-3061::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10668

EchoBASE (EcoliWiki Page)

EchoBASE:EB0662

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1335

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1808

EcoGene (EcoliWiki Page)

EcoGene:EG10668

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:OGT

CGSC (EcoliWiki Page)

CGSC:31984

ASAP (EcoliWikiPage)

ASAP:ABE-0004486


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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Ogt

Synonyms

O-6-alkylguanine-DNA:cysteine-protein methyltransferase[1], O-6-alkylguanine-DNA/Cysteine-protein-methyltransferase[2][1], B1335[2][1], Ogt[2][1]

Product description

Ogt[2][3]

O6-methylguanine-DNA methyltransferase, constitutive[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

QualifierGO IDGO term nameReference(s)Evidence Codewith/fromAspectNotesStatus

GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014048

F

Seeded from EcoCyc [7]

complete

GO:0003908

methylated-DNA-(protein)-cysteine S-methyltransferase activity

GO_REF:0000002
GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR001497
InterPro:IPR008332
EC:2.1.1.63

F

Seeded from EcoCyc [7]

complete

GO:0006281

DNA repair

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR001497
InterPro:IPR008332
InterPro:IPR014048
SP_KW:KW-0234

P

Seeded from EcoCyc [7]

complete

GO:0006974

response to DNA damage stimulus

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc [7]

complete

GO:0008168

methyltransferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0489

F

Seeded from EcoCyc [7]

complete

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [7]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0006306

DNA methylation

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009307

DNA restriction-modification system

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner TypePartnerNotesReferencesEvidence

Protein

aceF

PMID:15690043

Experiment(s):EBI-882987

Protein

lpdA

PMID:15690043

Experiment(s):EBI-882987, EBI-890521

Protein

aceE

PMID:15690043

Experiment(s):EBI-890521

Protein

gapA

PMID:15690043

Experiment(s):EBI-890521

Protein

groL

PMID:15690043

Experiment(s):EBI-890521

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

CompartmentDescriptionEvidenceSourceNotes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG YERISATNPG
GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI PCGQVMHYGQ LAEQLGRPGA
ARAVGAANGS NPISIVVPCH RVIGRNGTMT GYAGGVQRKE WLLRHEGYLL L
Length

171

Mol. Wt

19.178 kDa

pI

8.5 (calculated)

Extinction coefficient

32,430 - 32,805 (calc based on 7 Y, 4 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

TypeResiduesDescriptionNotesReferences
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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures
Models

View models at:

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Structure figures

Notes

Gene Product Resources

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Resource typeSourceNotes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10668-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AFH0

UniProt (EcoliWiki Page)

UniProt:P0AFH0

PFAM (EcoliWiki Page)

PFAM:PF02870

RefSeq (EcoliWiki Page)

RefSeq:NP_415851


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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

MoleculeOrganism or StrainCellular levelUnitsMediumTemperature °COther (e.g. anaerobic)Growth rateAssay usedNotesReference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ogt

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Figure courtesy of RegulonDB

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=1398240..1398280&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein+flip&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele NameMutationPhenotypeReference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

TypeReferenceNotes

microarray

GEO Profiles:b1335 (EcoliWiki Page)

NCBI GEO profiles for ogt

microarray

GenExpDB:b1335 (EcoliWiki Page)

Summary of data for ogt from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource NameResource TypeDescriptionSourceNotes
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Notes

Accessions Related to ogt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

DatabaseAccessionNotes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10668

EchoBASE (EcoliWiki Page)

EchoBASE:EB0662

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1335

EcoGene (EcoliWiki Page)

EcoGene:EG10668


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

OrganismHomologs (Statistics)Comments

Bos taurus

  • ENSBTAP00000012824 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000019538 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0232973 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000021537 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000131192 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00024953001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000000045 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

OGT

From SHIGELLACYC

E. coli O157

OGT

From ECOO157CYC

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Do-It-Yourself Web Tools

Notes

Families

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DatabaseAccessionNotes

PFAM (EcoliWiki Page)

PFAM:PF02870


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70

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