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pACYC184

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At a Glance

pACYC184, image BCCM/LMBP

Type

Cloning

Origin of Replication

p15A

Copy #

low, 15 copies/cell

Markers

Chloramphenicol Tetracycline

Link to Sequence

LMBP 992

Features
Accession Number

X06403



Notes

This multicopy plasmid is compatible with ColE1- or pMB1-derived plasmids.
pACYC184 can be amplified 10-fold in the presence of spectinomycin.
The chloramphenicol resistance gene is excisable as a HaeII fragment.

History

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Sources

BCCM/LMBP Plasmid Collection
Invitrogen

References

Chang et al., J. Bacteriol. 134 (1978), 1141-1156

Sequence

       1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
      61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
    1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
    1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
    1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
    1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
    1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
    1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
    1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
    1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
    1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
    1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
    1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
    1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
    1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
    1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
    1861 ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
    1921 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
    1981 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
    2041 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
    2101 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct
    2161 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
    2221 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
    2281 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
    2341 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
    2401 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
    2461 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
    2521 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
    2581 gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga
    2641 tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag
    2701 gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca
    2761 cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
    2821 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
    2881 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg
    2941 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
    3001 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
    3061 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
    3121 tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc
    3181 aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta
    3241 ttctgagtta caacagtccg caccgctgtc cggtagctcc ttccggtggg cgcggggcat
    3301 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
    3361 ggcagcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
    3421 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
    3481 acctacatct gtattaacga agcgctaacc gtttttatca ggctctggga ggcagaataa
    3541 atgatcatat cgtcaattat tacctccacg gggagagcct gagcaaactg gcctcaggca
    3601 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
    3661 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcgaa tttgctttcg
    3721 aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaccag gcgtttaagg
    3781 gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
    3841 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
    3901 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
    3961 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
    4021 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
    4081 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
    4141 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
    4201 aacactatcc catatcacca gctcaccgtc tttcattgcc atacg