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pACYCDuet-1

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At a Glance

Image of pACYCDuet-1. Novagen

Type

Expression

Origin of Replication

P15A

Copy #

10-12

Markers

chloramphenicol

Link to Sequence

Novagen

Features

T7 promoter
His•Tag
Multiple cloning sites
S•Tag
lacI coding sequence

Accession Number




Notes

This vector can be transformed into the same cell with pETDuet-1, pCDFDuet-1, and pRSFDuet-1 or pCOLADuet-1 in the appropriate host strain for the coexpression of up to eight target genes.[1][2].

History

pACYCDuet-1 is designed for the coexpression of two target genes [3].

Sources

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

  1. Novy, R., et all. 2002. inNovations 15,2.
  2. Held, D., et al. 2003. inNovations 18, 4.
  3. Tolia, NH & Joshua-Tor, L (2006) Strategies for protein coexpression in Escherichia coli. Nat. Methods 3 55-64 PubMed EcoliWiki page

Sequence

       1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag 
      61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag 
     121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag 
     181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt 
     241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata 
     301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt 
     361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg 
     421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg 
     481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg 
     541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt 
     601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat 
     661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct 
     721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt 
     781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc 
     841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc 
     901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa 
     961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca 
    1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat 
    1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc 
    1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca 
    1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag 
    1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc 
    1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt 
    1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt 
    1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat 
    1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat 
    1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct 
    1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc 
    1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc 
    1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc 
    1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt 
    1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc 
    1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac 
    1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga 
    2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag 
    2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt 
    2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc 
    2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg 
    2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc 
    2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt 
    2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt 
    2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt 
    2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc 
    2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca 
    2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga 
    2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag 
    2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 
    2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca 
    2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc 
    2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt 
    3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta 
    3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg 
    3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca 
    3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa 
    3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac 
    3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc 
    3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga 
    3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt 
    3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg 
    3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca 
    3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac 
    3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt 
    3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa 
    3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg 
    3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc 
    3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc 
    3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata