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pALTER-EX1

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At a Glance

pALTER-EX1, Key: red=ampicillin marker, magenta=promoter, cyan=MCS, Image provided by Promega

Type

Expression

Origin of Replication

f1, pUC, M13

Copy #
Markers

Chloramphenicol acetyltransferase, Ampicillin, Tetracycline

Link to Sequence

NCBI

Features

T7 RNA polymerase promoter
SP6 RNA polymerase promoter
tac promoter
multiple cloning site
lacZ start codon
lac operon sequences
lac operator
TetR gene coding region
EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, PstI, SphI, HindIII

Accession Number

U47102



Notes

This vector is tetracyline resistant and ampicillin sensitive as supplied by Promega. Contains a novel polylinker that allows protein expression in vivo and in vitro.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Promega Discontinued

References

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Sequence

1  GGGCGAATTC GTTAACTTTA AGAAGGAGAT ATACCCATGG AGGCCTCTAG
51  ACTGCAGCGG CCGCATGCAT ATGGATCCTG TTTCCTGTGT GAAATTGTTA
101  TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG
151  GGGGATGGGG AGTAAGCTTG AGTATTCTAT AGTGTCACCT AAATAGCTTG
201  GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
251  AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG
301  CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
351  TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
401  CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
451  CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
501  TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
551  GAACATGAAT TAATTCTCAT GTTTGACAGC TTATCATCGG ATAACCCCTT
601  GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGTTCTCAT GTTTGACAGC
651  TTATCTTCGA TTAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA
701  CGCAGTCAGG CACCGTGTAT GAAATCTAAC AATGCGCTCA TCGTCATCCT
751  CGGCACCGTC ACCCTGGATG CTGTAGGCAT AGGCTTGGTT ATGCCGGTAC
801  TGCCGGGCCT CTTGCGGGAT ATCGTCCATT CCGACAGCAT CGCCAGTCAC
851  TATGGCGTGC TGCTAGCGCT ATATGCGTTG ATGCAATTTC TATGCGCACC
901  CGTTCTCGGA GCACTGTCCG ACCGCTTTGG CCGCCGCCCA GTCCTGCTCG
951  CTTCGCTACT TGGAGCCACT ATCGACTACG CGATCATGGC GACCACACCC
1001  GTCCTGTGGA TTCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC
1051  CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG
1101  ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG
1151  GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTACATGC
1201  ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT
1251  GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGCCGACC CATGCCCTTG
1301  AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT
1351  CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG
1401  TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC
1451  GCGACGATGA TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT
1501  CGCTCAAGCC TTCGTCACTG GTCCCGCCAC CAAACGTTTC GGCGAGAAGC
1551  AGGCCATTAT CGCCGGCATG GCGGCCGACG CGCTGGGCTA CGTCTTGCTG
1601  GCGTTCGCGA CGCGAGGCTG GATGGCCTTC CCCATTATGA TTCTTCTCGC
1651  TTCCGGCGGC ATCGGGATGC CCGCGTTGCA GGCCATGCTG TCCAGGCAGG
1701  TAGATGACGA CCATCAGGGA CAGCTTCAAG GATCGCTCGC GGCTCTTACC
1751  AGCCTAACTT CGATCATTGG ACCGCTGATC GTCACGGCGA TTTATGCCGC
1801  CTCGGCGAGC ACATGGAACG GGTTGGCATG GATTGTAGGC GCCGCCCTAT
1851  ACCTTGTCTG CCTCCCCGCG TTGCGTCGCG GTGCATGGAG CCGGGCCACC
1901  TCGACCTGAA TGGAAGCCGG CGGCACCTCG CTAACGGATT CACCACTCCA
1951  AGAATTGGAG CCAATCAATT CTTGCGGAGA ACTGTGAATG CGCAAACCAA
2001  CCCTTGGCAG AACATATCCA TCGCGTCCGC CATCTCCAGC AGCCGCACGC
2051  GGCGCATCTC GGGCAGCGTT GGGTCCTGGC CACGGGTGCG CATGATCGTG
2101  CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA
2151  GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA
2201  AAACGTCTGC GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT
2251  TCGTAAAGTC TGGAAACGCG GAAGTCAGCG CCCTGCACCA TTATGTTCCG
2301  GATCTGCATC GCAGGATGCT GCTGGCTACC CTGTGGAACA CCTACATCTG
2351  TATTAACGAA GCGCTGGCAT TGACCCTGAG TGATTTTTCT CTGGTCCCGC
2401  CGCATCCATA CCGCCAGTTG TTTACCCTCA CAACGTTCCA GTAACCGGGC
2451  ATGTTCATCA TCAGTAACCC GTATCGTGAG CATCCTCTCT CGTTTCATCG
2501  GTATCATTAC CCCCATGAAC AGAAATCCCC CTTACACGGA GGCATCAGTG
2551  ACCAAACAGG AAAAAACCGC CCTTAACATG GCCCGCTTTA TCAGAAGCCA
2601  GACATTAACG CTTCTGGAGA AACTCAACGA GCTGGACGCG GATGAACAGG
2651  CAGACATCTG TGAATCGCTT CACGACCACG CTGATGAGCT TTACCGCAGC
2701  TGCCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT
2751  CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG
2801  CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG
2851  ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA
2901  TCAGAGCAGA TTGTACTGAG AGTGCACCCT ATGCGGTGTG AAATACCGCA
2951  CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC
3001  TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
3051  CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG
3101  AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG
3151  CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
3201  AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
3251  ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
3301  CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
3351  GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT
3401  TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
3451  GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
3501  TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
3551  TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
3601  CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
3651  GGAAAAAGCG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG
3701  CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT
3751  CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
3801  GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
3851  CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
3901  TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
3951  CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC
4001  CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG
4051  CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
4101  ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
4151  ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
4201  GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC GGCATCGTGG
4251  TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
4301  TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
4351  CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC
4401  TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
4451  AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
4501  GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA
4551  CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
4601  TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
4651  GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA
4701  GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
4751  ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
4801  TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
4851  AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
4901  AAAGTGCCAC CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA
4951  TAAAAATAGG CGTATCACGA GGCCCTTTCG TCTCGCGCGT TTCGGTGATG
5001  ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT
5051  CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG
5101  TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG
5151  TACTGAGAGT GCACCCTATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG
5201  AGAAAATACC GCATCAGGAA ATTGTAAGCG TTAATATTTT GTTAAAATTC
5251  GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
5301  CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG
5351  TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC
5401  GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC
5451  ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC
5501  GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG
5551  AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
5601  GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC
5651  TTAATGCGCC GCTACAGGGC GCGTCCATTC GCCATTCAGG CTGCGCAACT
5701  GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
5751  AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC
5801  CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGTAA TACGACTCAC
5851  TATA