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pALTER-EX2

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At a Glance

pALTER-EX2, Key: magenta=promoter, cyan=MCS, green=Chloramphenicol acetyltransferase region, yellow=Tetracycline, Image provided by Promega

Type

Expression

Origin of Replication

p15a, pUC, M13, f1

Copy #
Markers

Chloramphenicol acetyltransferase, Tetracycline, Ampicillin

Link to Sequence

NCBI

Features

T7 RNA polymerase promoter
SP6 RNA polymerase promoter
tac promoter
multiple cloning sites
lacZ start codon
lac operon sequences
lac operator
EcoRI, HpaI, NcoI, StuI, PstI, NotI, SphI, NsiI, NdeI, BamHI, HindIII

Accession Number

U47103



Notes

This vector is tetracycline resistant and chloramphenicol sensitive. Has been discontinued by Promega.

History

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Sources

Promega

References

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Sequence

1  GGGCGAATTC GTTAACTTTA AGAAGGAGAT ATACCCATGG AGGCCTCTAG
51  ACTGCAGCGG CCGCATGCAT ATGGATCCTG TTTCCTGTGT GAAATTGTTA
101  TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG
151  GGGGATGGGG AGTAAGCTTG AGTATTCTAT AGTGTCACCT AAATAGCTTG
201  GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
251  AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG
301  CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
351  TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
401  CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
451  CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
501  TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
551  GAACATGAAT TAATTCTCAT GTTTGACAGC TTATCATCGG ATAACCCCTT
601  GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGTTCTCAT GTTTGACAGC
651  TTATCTTCGA TTAGCTTTAA TGCGGTAGTT TATCACAGTT AAATTGCTAA
701  CGCAGTCAGG CACCGTGTAT GAAATCTAAC AATGCGCTCA TCGTCATCCT
751  CGGCACCGTC ACCCTGGATG CTGTAGGCAT AGGCTTGGTT ATGCCGGTAC
801  TGCCGGGCCT CTTGCGGGAT ATCGTCCATT CCGACAGCAT CGCCAGTCAC
851  TATGGCGTGC TGCTAGCGCT ATATGCGTTG ATGCAATTTC TATGCGCACC
901  CGTTCTCGGA GCACTGTCCG ACCGCTTTGG CCGCCGCCCA GTCCTGCTCG
951  CTTCGCTACT TGGAGCCACT ATCGACTACG CGATCATGGC GACCACACCC
1001  GTCCTGTGGA TTCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC
1051  CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG
1101  ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG
1151  GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTACATGC
1201  ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT
1251  GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGCCGACC CATGCCCTTG
1301  AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT
1351  CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG
1401  TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC
1451  GCGACGATGA TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT
1501  CGCTCAAGCC TTCGTCACTG GTCCCGCCAC CAAACGTTTC GGCGAGAAGC
1551  AGGCCATTAT CGCCGGCATG GCGGCCGACG CGCTGGGCTA CGTCTTGCTG
1601  GCGTTCGCGA CGCGAGGCTG GATGGCCTTC CCCATTATGA TTCTTCTCGC
1651  TTCCGGCGGC ATCGGGATGC CCGCGTTGCA GGCCATGCTG TCCAGGCAGG
1701  TAGATGACGA CCATCAGGGA CAGCTTCAAG GATCGCTCGC GGCTCTTACC
1751  AGCCTAACTT CGATCATTGG ACCGCTGATC GTCACGGCGA TTTATGCCGC
1801  CTCGGCGAGC ACATGGAACG GGTTGGCATG GATTGTAGGC GCCGCCCTAT
1851  ACCTTGTCTG CCTCCCCGCG TTGCGTCGCG GTGCATGGAG CCGGGCCACC
1901  TCGACCTGAA TGGAAGCCGG CGGCACCTCG CTAACGGATT CACCACTCCA
1951  AGAATTGGAG CCAATCAATT CTTGCGGAGA ACTGTGAATG CGCAAACCAA
2001  CCCTTGGCAG AACATATCCA TCGCGTCCGC CATCTCCAGC AGCCGCACGC
2051  GGCGCATCTC GGGCAGCGTT GGGTCCTGGC CACGGGTGCG CATGATCGTG
2101  CTCCTGTCGT TGAGGACCCG GCTAGGCTGG CGGGGTTGCC TTACTGGTTA
2151  GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC TGCTGCTGCA
2201  AAACGTCTGC GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT
2251  TCGTAAAGTC TGGAAACGCG GAAGTCCCCT ACGTGCTGCT GAAGTTGCCC
2301  GCAACAGAGA GTGGAACCAA CCGGTGATAC CACGATACTA TGACTGAGAG
2351  TCAACGCCAT GAGCGGCCTC ATTTCTTATT CTGAGTTACA ACAGTCCGCA
2401  CCGCTGCCGG TAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC
2451  GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC
2501  AGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA
2551  AACAAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC AGGATGCTGC
2601  TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTAACCGTT
2651  TTTATCAGGC TCTGGGAGGC AGAATAAATG ATCATATCGT CAATTATTAC
2701  CTCCACGGGG AGAGCCTGAG CAAACTGGCC TCAGGCATTT GAGAAGCACA
2751  CGGTCACACT GCTTCCGGTA GTCAATAAAC CGGTAAACCA GCAATAGACA
2801  TAAGCGGCTA TTTAACGACC CTGCCCTGAA CCGACGACCG GGTCGAATTT
2851  GCTTTCGAAT TTCTGCCATT CATCCGCTTA TTATCACTTA TTCAGGCGTA
2901  GCAACCAGGC GTTTAAGGGC ACCAATAACT GCCTTAAAAA AATTACGCCC
2951  CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG CATTCTGCCG
3001  ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT
3051  CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATCGTG AAAACGGGGG
3101  CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC
3151  ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG
3201  GAAATAGGCC AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT
3251  GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG CGATGAAAAC
3301  GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA
3351  TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACCGG ATGAGCATTC
3401  ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT
3451  TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT
3501  TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA
3551  TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT
3601  TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG
3651  GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCGA
3701  TCAACGTCTC ATTTTCGCCA AAAGTTGGCC CAGGGCTTCC CGGTATCAAC
3751  AGGGACACCA GGATTTATTT ATTCTGCGAA GTGATCTTCC GTCACAGGTA
3801  TTTATTCGGC GCAAAGTGCG TCGGGTGATG CTGCCAACTT ACTGATTTAG
3851  TGTATGATGG TGTTTTTGAG GTGCTCCAGT GGCTTCTGTT TCTATCAGCT
3901  GTCCCTCCTG TTCAGCTACT GACGGGGTGG TGCGTAACGG CAAAAGCACC
3951  GCCGGACATC AGCGCTAGCG GAGTGTATAC TGGCTTACTA TGTTGGCACT
4001  GATGAGGGTG TCAGTGAAGT GCTTCATGTG GCAGGAGAAA AAAGGCTGCA
4051  CCGGTGCGTC AGCAGAATAT GTGATACAGG ATATATTCCG CTTCCTCGCT
4101  CACTGACTCG CTACGCTCGG TCGTTCGACT GCGGCGAGCG GAAATGGCTT
4151  ACGAACGGGG CGGAGATTTC CTGGAAGATG CCAGGAAGAT ACTTAACAGG
4201  GAAGTGAGAG GGCCGCGGCA AAGCCGTTTT TCCATAGGCT CCGCCCCCCT
4251  GACAAGCATC ACGAAATCTG ACGCTCAAAT CAGTGGTGGC GAAACCCGAC
4301  AGGACTATAA AGATACCAGG CGTTTCCCCC TGGCGGCTCC CTCGTGCGCT
4351  CTCCTGTTCC TGCCTTTCGG TTTACCGGTG TCATTCCGCT GTTATGGCCG
4401  CGTTTGTCTC ATTCCACGCC TGACACTCAG TTCCGGGTAG GCAGTTCGCT
4451  CCAAGCTGGA CTGTATGCAC GAACCCCCCG TTCAGTCCGA CCGCTGCGCC
4501  TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGAAAGAC ATGCAAAAGC
4551  ACCACTGGCA GCAGCCACTG GTAATTGATT TAGAGGAGTT AGTCTTGAAG
4601  TCATGCGCCG GTTAAGGCTA AACTGAAAGG ACAAGTTTTG GTGACTGCGC
4651  TCCTCCAAGC CAGTTACCTC GGTTCAAAGA GTTGGTAGCT CAGAGAACCT
4701  TCGAAAAACC GCCCTGCAAG GCGGTTTTTT CGTTTTCAGA GCAAGAGATT
4751  ACGCGCAGAC CAAAACGATC TCAAGAAGAT CATCTTATTA ATCAGATAAA
4801  ATATTTCTAG ATTTCAGTGC AATTTATCTC TTCAAATGTA GCACCTGAAG
4851  TCAGCCCCAT ACGATATAAG TTGTAATTCT CATGTTTGAC AGCTTATCAT
4901  CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
4951  CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA
5001  CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
5051  GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG
5101  GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTAGAGAG TGCACCCTAT
5151  GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGA
5201  AATTGTAAGC GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA
5251  TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT CCCTTATAAA
5301  TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA
5351  GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG CGAAAAACCG
5401  TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT
5451  TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AAGGGAGCCC
5501  CCGATTTAGA GCTTGACGGG GAAAGCCGGC GAACGTGGCG AGAAAGGAAG
5551  GGAAGAAAGC GAAAGGAGCG GGCGCTAGGG CGCTGGCAAG TGTAGCGGTC
5601  ACGCTGCGCG TAACCACCAC ACCCGCCGCG CTTAATGCGC CGCTACAGGG
5651  CGCGTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT
5701  GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA
5751  GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA
5801  ACGACGGCCA GTGAATTGTA ATACGACTCA CTATA