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pBAC-2cp

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At a Glance

pBAC-2cp, Image made with PlasMapper

Type

Cloning, Expression

Origin of Replication

f1

Copy #
Markers

Ampicillin

Link to Sequence

Novagen

Features

polh Promoter
His Tag
S Tag
Enterokinase Protease Cleavage Site
ATG Start Codon
MCS

Accession Number




Notes

The pBAC-2cp Ek/LIC vector is an Ek/LIC version of pBAC-2cp. Ligation-independent cloning (LIC) was developed for directional cloning of PCR products without restriction enzyme digestion or ligation reactions[1][2]. The pBAC-2cp Ek/LIC vector is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. A Ligation-Independent Cloning (LIC) version of the vector is available for rapid, directional cloning of PCR products adjacent to the enterokinase cleavage site.

History

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Sources

Novagen

References

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See Help:References for how to manage references in EcoliWiki.

  1. Aslanidis, C & de Jong, PJ (1990) Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18 6069-74 PubMed EcoliWiki page
  2. Haun, RS et al. (1992) Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors. BioTechniques 13 515-8 PubMed EcoliWiki page

Sequence

        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aaccatctcg 
     1201 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata 
     1261 tgcccatggt ccatcatcac caccatcact ccgcgggttt ggtgcccaga ggcagcggca 
     1321 aagaaacggc ggcggcgaaa tttgaacgcc aacacatgga cagcgctagc ggtggaggtg 
     1381 atgacgacga caagagcccg ggcttctcct caaaaggcct ggatccgaat tcgagctcga 
     1441 agcttgcggc cgcactcgag caccaccacc accaccacta acctaggtag ctgagcgcat 
     1501 gcaagctgat ccgggttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt 
     1561 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta 
     1621 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc 
     1681 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc 
     1741 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag 
     1801 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc 
     1861 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact 
     1921 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt 
     1981 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt 
     2041 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt 
     2101 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac 
     2161 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat 
     2221 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg 
     2281 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga 
     2341 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact 
     2401 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct 
     2461 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg 
     2521 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg 
     2581 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt 
     2641 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg 
     2701 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa 
     2761 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa 
     2821 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg gatcgatccc 
     2881 gcacgccgat aacaagcctt ttcattttta ctacagcatt gtagtggcga gacacttcgc 
     2941 tgtcgtcgag gtttaaacgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 
     3001 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 
     3061 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 
     3121 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 
     3181 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 
     3241 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 
     3301 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 
     3361 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 
     3421 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 
     3481 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 
     3541 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 
     3601 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 
     3661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 
     3721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 
     3781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 
     3841 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 
     3901 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 
     3961 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 
     4021 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 
     4081 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 
     4141 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 
     4201 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 
     4261 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 
     4321 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 
     4381 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 
     4441 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 
     4501 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 
     4561 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 
     4621 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 
     4681 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 
     4741 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 
     4801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 
     4861 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     4921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     4981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     5041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     5101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     5161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     5221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     5281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 
     5341 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 
     5401 ctattacgcc a