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pBEn-SBP-SET1c

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At a Glance

pBEn-SBP-SET1c, Image provided by Agilent Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac operator
extended RBS
SET1 tag
SBP
MCS
lacI repressor ORF

Accession Number




Notes

Use when you want to use the VariFlex tags at the N-terminus of your protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

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Sequence

    1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
   51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
  101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
  151  CGCACGCACC CGCGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG  
  201  CTGCCACCGC TGAACAAACT AGTGACGAGA AGACCACCGG CTGGCGGGGC  
  251  GGCCACGTGG TGGAGGGCCT GGCCGGCGAG CTGGAGCAGC TGCGGGCCAG  
  301  GCTGGAGCAC CACCCTCAGG GCCAGCGGGA GCCCTCCGGC GGCTGCAAGC  
  351  TGGGCCTGCT GGTTCCGCGT GGATCTGCGG ATCCGAATTC TCTTCCCGGG  
  401  TCTTGTTCCA TGGGTCGACT CGAGCTCAAG CTTAGATCCG GCTGCTAACA  
  451  AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA  
  501  GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA  
  551  AGGAGGAACT ATATCCGGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT  
  601  AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA  
  651  TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT  
  701  AACTGTGATA AACTACCGCA TTAAAGCTAA TCGAAGATAA GCTGTCAAAC  
  751  ATGACAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG  
  801  GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG  
  851  GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA  
  901  ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT  
  951  TGAAAAAGGA ACAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC  
 1001  CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG  
 1051  TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC  
 1101  GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA  
 1151  ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT  
 1201  TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT  
 1251  TCTCAGAATG ACTTGGTTGA CGCGTCACCA GTCACAGAAA AGCATCTTAC  
 1301  GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG  
 1351  ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG  
 1401  CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG  
 1451  TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA  
 1501  CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA  
 1551  CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA  
 1601  TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA  
 1651  TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA  
 1701  GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC  
 1751  GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG  
 1801  GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT  
 1851  ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT  
 1901  GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT  
 1951  CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA  
 2001  GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC  
 2051  GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC  
 2101  CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA  
 2151  GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC  
 2201  ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA  
 2251  AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG  
 2301  CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG  
 2351  AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG  
 2401  CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG  
 2451  GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA  
 2501  TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT  
 2551  TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG  
 2601  GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT  
 2651  TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT  
 2701  GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG  
 2751  AGCGAGGAAG CGGAAGACCG CCTGATGCGG TATTTTCTCC TTACGCATCT  
 2801  GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC  
 2851  TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG  
 2901  GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC  
 2951  GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC  
 3001  GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG  
 3051  GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT  
 3101  CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT  
 3151  GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT  
 3201  GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA  
 3251  TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA  
 3301  CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT  
 3351  GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA  
 3401  ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG  
 3451  ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA  
 3501  CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG  
 3551  TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC  
 3601  TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG  
 3651  GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC  
 3701  GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA  
 3751  GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT  
 3801  TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT  
 3851  CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA  
 3901  GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC  
 3951  GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG  
 4001  GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT  
 4051  CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG  
 4101  GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA  
 4151  CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG  
 4201  CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA  
 4251  GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT  
 4301  CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG  
 4351  GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG  
 4401  ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC  
 4451  TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA  
 4501  CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG  
 4551  TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG  
 4601  TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC  
 4651  TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC  
 4701  GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG  
 4751  GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA  
 4801  TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG  
 4851  CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT  
 4901  TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT  
 4951  TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA  
 5001  GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA  
 5051  GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC  
 5101  GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA  
 5151  GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA  
 5201  TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT  
 5251  TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG  
 5301  TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT  
 5351  TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG  
 5401  GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG  
 5451  GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG  
 5501  TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA  
 5551  TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC  
 5601  ATTCACCACC CTGAATTGAC TGTCTTCCGG GCGCTATCAT GCCATACCGC  
 5651  GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT  
 5701  ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA  
 5751  GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT  
 5801  CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT  
 5851  GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT  
 5901  AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT  
 5951  CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT