PortEco logo

pBEn-SBP-SET2b

From EcoliWiki
Jump to: navigation, search

At a Glance

pBEn-SBP-SET2b Image provided by Agilent-genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac Operator
extended RBS
SET2 tag
SBP tag
MCS
lacI repressor ORF

Accession Number




Notes

Use when you want to use the VariFlex tags at the N-terminus of your protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCAGAGACC GAAGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGAGG  
 201  CTGAAACCGC TGAACAAACT AGTGACGAGA AGACCACCGG CTGGCGGGGC  
 251  GGCCACGTGG TGGAGGGCCT GGCCGGCGAG CTGGAGCAGC TGCGGGCCAG  
 301  GCTGGAGCAC CACCCTCAGG GCCAGCGGGA GCCCTCCGGC GGCTGCAAGC  
 351  TGGGCCTGCT GGTTCCGCGT GGATCTCGGA TCCGAATTCT CTTCCCGGGT  
 401  CTTGTTCCAT GGGTCGACTC GAGCTCAAGC TTAGATCCGG CTGCTAACAA  
 451  AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG  
 501  CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA  
 551  GGAGGAACTA TATCCGGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA  
 601  ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT  
 651  GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA  
 701  ACTGTGATAA ACTACCGCAT TAAAGCTAAT CGAAGATAAG CTGTCAAACA  
 751  TGACAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG  
 801  TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG  
 851  GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA  
 901  TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT  
 951  GAAAAAGGAA CAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC  
1001  TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT  
1051  GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG  
1101  AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA  
1151  CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT  
1201  ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT  
1251  CTCAGAATGA CTTGGTTGAC GCGTCACCAG TCACAGAAAA GCATCTTACG  
1301  GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA  
1351  TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC  
1401  TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT  
1451  TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC  
1501  GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC  
1551  TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT  
1601  AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT  
1651  TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG  
1701  CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG  
1751  GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG  
1801  TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA  
1851  TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG  
1901  AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC  
1951  GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG  
2001  ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG  
2051  CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC  
2101  GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG  
2151  TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA  
2201  TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA  
2251  GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC  
2301  AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA  
2351  ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC  
2401  CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG  
2451  TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT  
2501  CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT  
2551  GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG  
2601  CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT  
2651  CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG  
2701  AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA  
2751  GCGAGGAAGC GGAAGACCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG  
2801  TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT  
2851  GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG  
2901  GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG  
2951  GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG  
3001  GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG  
3051  CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC  
3101  TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG  
3151  TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG  
3201  GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT  
3251  ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC  
3301  ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG  
3351  CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA  
3401  TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA  
3451  TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC  
3501  GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT  
3551  TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT  
3601  GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG  
3651  AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG  
3701  CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG  
3751  GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT  
3801  CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC  
3851  GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG  
3901  GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG  
3951  AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG  
4001  CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC  
4051  TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG  
4101  AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC  
4151  GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC  
4201  CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG  
4251  CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC  
4301  GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG  
4351  CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA  
4401  CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT  
4451  CTCAAGGGCA TCGGTCGAGA TCCCGGTGCC TAATGAGTGA GCTAACTTAC  
4501  ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT  
4551  GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT   
4601  ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT  
4651  GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG  
4701  CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG  
4751  GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATAT  
4801  CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC  
4851  GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT  
4901  CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT  
4951  CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG  
5001  CCAGCCAGAC GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG  
5051  CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG  
5101  TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG  
5151  ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT  
5201  GGCATCCTGG TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT  
5251  GCGCGAGAAG ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT  
5301  TCTACCATCG ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT  
5351  AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG  
5401  CAACGCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG  
5451  TTGGGAATGT AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT  
5501  TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT  
5551  AAGAGACACC GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCACA  
5601  TTCACCACCC TGAATTGACT GTCTTCCGGG CGCTATCATG CCATACCGCG  
5651  AAAGGTTTTG CGCCATTCGA TGGTGTCCGG GATCTCGACG CTCTCCCTTA  
5701  TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG  
5751  CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC  
5801  CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG  
5851  AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA  
5901  GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC  
5951  CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT