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pBEn-SBP-SET2c

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At a Glance

pBEn-SBP-SET2c Image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac Operator
extended RBS
SET2 tag
SBP tag
MCS
lacI repressor ORF

Accession Number




Notes

Use when you want to use the VariFlex tags at N-terminus of protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

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Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCAGAGACC GAAGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGAGG  
 201  CTGAAACCGC TGAACAAACT AGTGACGAGA AGACCACCGG CTGGCGGGGC  
 251  GGCCACGTGG TGGAGGGCCT GGCCGGCGAG CTGGAGCAGC TGCGGGCCAG  
 301  GCTGGAGCAC CACCCTCAGG GCCAGCGGGA GCCCTCCGGC GGCTGCAAGC  
 351  TGGGCCTGCT GGTTCCGCGT GGATCTGCGG ATCCGAATTC TCTTCCCGGG  
 401  TCTTGTTCCA TGGGTCGACT CGAGCTCAAG CTTAGATCCG GCTGCTAACA  
 451  AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA  
 501  GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA  
 551  AGGAGGAACT ATATCCGGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT  
 601  AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA  
 651  TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT  
 701  AACTGTGATA AACTACCGCA TTAAAGCTAA TCGAAGATAA GCTGTCAAAC  
 751  ATGACAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG  
 801  GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG  
 851  GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA  
 901  ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT  
 951  TGAAAAAGGA ACAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC  
1001  CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG  
1051  TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC  
1101  GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA  
1151  ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT  
1201  TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT  
1251  TCTCAGAATG ACTTGGTTGA CGCGTCACCA GTCACAGAAA AGCATCTTAC  
1301  GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG  
1351  ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG  
1401  CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG  
1451  TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA  
1501  CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA  
1551  CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA  
1601  TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA  
1651  TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA  
1701  GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC  
1751  GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG  
1801  GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT  
1851  ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT  
1901  GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT  
1951  CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA  
2001  GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC  
2051  GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC  
2101  CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA  
2151  GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC  
2201  ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA  
2251  AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG  
2301  CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG  
2351  AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG  
2401  CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG  
2451  GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA  
2501  TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT  
2551  TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG  
2601  GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT  
2651  TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT  
2701  GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG  
2751  AGCGAGGAAG CGGAAGACCG CCTGATGCGG TATTTTCTCC TTACGCATCT  
2801  GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC  
2851  TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG  
2901  GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC  
2951  GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC  
3001  GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG  
3051  GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT  
3101  CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT  
3151  GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT  
3201  GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA  
3251  TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA  
3301  CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT  
3351  GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA  
3401  ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG  
3451  ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA  
3501  CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG  
3551  TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC  
3601  TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG  
3651  GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC  
3701  GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA  
3751  GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT  
3801  TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT  
3851  CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA  
3901  GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC  
3951  GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG  
4001  GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT  
4051  CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG  
4101  GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA  
4151  CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG  
4201  CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA  
4251  GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT  
4301  CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG  
4351  GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG  
4401  ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC  
4451  TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA  
4501  CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG  
4551  TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG  
4601  TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC  
4651  TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC  
4701  GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG  
4751  GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA  
4801  TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG  
4851  CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT  
4901  TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT  
4951  TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA  
5001  GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA  
5051  GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC  
5101  GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA  
5151  GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA  
5201  TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT  
5251  TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG  
5301  TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT  
5351  TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG  
5401  GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG  
5451  GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG  
5501  TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA  
5551  TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC  
5601  ATTCACCACC CTGAATTGAC TGTCTTCCGG GCGCTATCAT GCCATACCGC  
5651  GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT  
5701  ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA  
5751  GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT  
5801  CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT  
5851  GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT  
5901  AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT  
5951  CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT