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pBEn-SBP-SET3a

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At a Glance

pBEn-SBP-SET3a Image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac Operator
extended RBS
SET3 tag
SBP tag
MCS
lacI repressor ORF

Accession Number




Notes

Use pBEn vectors when want to use VariFlex tags at N-terminus of protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

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Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCAGAGACC GAAGCTGCTA ACAAAGCCGA AGAGGAAGCT GAGTTGGAGG  
 201  CTGAAACCGC TGAACAAACT AGTGACGAGA AGACCACCGG CTGGCGGGGC  
 251  GGCCACGTGG TGGAGGGCCT GGCCGGCGAG CTGGAGCAGC TGCGGGCCAG  
 301  GCTGGAGCAC CACCCTCAGG GCCAGCGGGA GCCCTCCGGC GGCTGCAAGC  
 351  TGGGCCTGCT GGTTCCGCGT GGATCTGGAT CCGAATTCTC TTCCCGGGTC  
 401  TTGTTCCATG GGTCGACTCG AGCTCAAGCT TAGATCCGGC TGCTAACAAA  
 451  GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC  
 501  ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG  
 551  GAGGAACTAT ATCCGGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA  
 601  CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG  
 651  AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA  
 701  CTGTGATAAA CTACCGCATT AAAGCTAATC GAAGATAAGC TGTCAAACAT  
 751  GACAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT  
 801  TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG  
 851  GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT  
 901  ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG  
 951  AAAAAGGAAC AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT  
1001  TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG  
1051  AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA  
1101  ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC  
1151  GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA  
1201  TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC  
1251  TCAGAATGAC TTGGTTGACG CGTCACCAGT CACAGAAAAG CATCTTACGG  
1301  ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT  
1351  AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT  
1401  AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT  
1451  GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG  
1501  ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT  
1551  ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA  
1601  AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT  
1651  GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC  
1701  ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG  
1751  GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT  
1801  GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT  
1851  ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA  
1901  AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG  
1951  TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA  
2001  TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC  
2051  TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG  
2101  AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT  
2151  GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT  
2201  ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG  
2251  TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA  
2301  GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA  
2351  CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC  
2401  ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT  
2451  CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC  
2501  TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG  
2551  TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC  
2601  CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC  
2651  CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA  
2701  GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG  
2751  CGAGGAAGCG GAAGACCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT  
2801  GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG  
2851  ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG  
2901  TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG  
2951  GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG  
3001  GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC  
3051  AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT  
3101  GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT  
3151  CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG  
3201  TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA  
3251  CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA  
3301  TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC  
3351  GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT  
3401  ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT  
3451  CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG  
3501  AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT  
3551  TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG  
3601  CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA  
3651  GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC  
3701  CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG  
3751  GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC  
3801  TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG  
3851  AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG  
3901  TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA  
3951  GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC  
4001  TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT  
4051  ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA  
4101  AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG  
4151  CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC  
4201  TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC  
4251  GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG  
4301  CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC  
4351  ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC  
4401  GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC  
4451  TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG CTAACTTACA  
4501  TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG  
4551  CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA  
4601  TTGGGCGCCA GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG  
4651  ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG TTGCAGCAAG CGGTCCACGC  
4701  TGGTTTGCCC CAGCAGGCGA AAATCCTGTT TGATGGTGGT TAACGGCGGG  
4751  ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA CCGAGATATC  
4801  CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG  
4851  CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC  
4901  AGCATTTGCA TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC  
4951  CCGTTCCGCT ATCGGCTGAA TTTGATTGCG AGTGAGATAT TTATGCCAGC  
5001  CAGCCAGACG CAGACGCGCC GAGACAGAAC TTAATGGGCC CGCTAACAGC  
5051  GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC CCAGTCGCGT  
5101  ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA  
5151  CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG  
5201  GCATCCTGGT CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG  
5251  CGCGAGAAGA TTGTGCACCG CCGCTTTACA GGCTTCGACG CCGCTTCGTT  
5301  CTACCATCGA CACCACCACG CTGGCACCCA GTTGATCGGC GCGAGATTTA  
5351  ATCGCCGCGA CAATTTGCGA CGGCGCGTGC AGGGCCAGAC TGGAGGTGGC  
5401  AACGCCAATC AGCAACGACT GTTTGCCCGC CAGTTGTTGT GCCACGCGGT  
5451  TGGGAATGTA ATTCAGCTCC GCCATCGCCG CTTCCACTTT TTCCCGCGTT  
5501  TTCGCAGAAA CGTGGCTGGC CTGGTTCACC ACGCGGGAAA CGGTCTGATA  
5551  AGAGACACCG GCATACTCTG CGACATCGTA TAACGTTACT GGTTTCACAT  
5601  TCACCACCCT GAATTGACTG TCTTCCGGGC GCTATCATGC CATACCGCGA  
5651  AAGGTTTTGC GCCATTCGAT GGTGTCCGGG ATCTCGACGC TCTCCCTTAT  
5701  GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC  
5751  ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC  
5801  CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA  
5851  GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG  
5901  GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC  
5951  GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT