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pBEn-SBPa

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At a Glance

pBEn-SBPa, image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 promoter
lac operator
SBP tag[1]
Multiple Cloning Site (MCS)
T7 terminator
lacI repressor ORF

Accession Number




Notes

The SBP tag, a synthetic sequence isolated from a random peptide library, has a high affinity for streptavidin resin (~2 × 10-9 M), and can be effectively eluted from the resin with biotin.

History

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Sources

Agilent-Genomics

References

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See Help:References for how to manage references in EcoliWiki.

  1. Keefe, AD et al. (2001) One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag. Protein Expr. Purif. 23 440-6 PubMed EcoliWiki page

[1] [2]

Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACGAGAAG  
 101  ACCACCGGCT GGCGGGGCGG CCACGTGGTG GAGGGCCTGG CCGGCGAGCT  
 151  GGAGCAGCTG CGGGCCAGGC TGGAGCACCA CCCTCAGGGC CAGCGGGAGC  
 201  CCTCCGGCGG CTGCAAGCTG GGCCTGCTGG TTCCGCGTGG ATCTGGATCC  
 251  GAATTCTCTT CCCGGGTCTT GTTCCATGGG TCGACTCGAG CTCAAGCTTA  
 301  GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC  
 351  CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA  
 401  GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC CGCAAGAGGC  
 451  CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG  
 501  GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG  
 551  ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTAATCGA  
 601  AGATAAGCTG TCAAACATGA CAATTCTTGA AGACGAAAGG GCCTCGTGAT  
 651  ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT  
 701  CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT  
 751  TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA  
 801  AATGCTTCAA TAATATTGAA AAAGGAACAG TATGAGTATT CAACATTTCC  
 851  GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT  
 901  CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC  
 951  ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA  
1001  GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG  
1051  CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG  
1101  TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGACGCG TCACCAGTCA  
1151  CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT  
1201  GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT  
1251  CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG  
1301  TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC  
1351  GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA  
1401  ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG  
1451  ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT  
1501  CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC  
1551  TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG  
1601  TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA  
1651  CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA  
1701  CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT  
1751  TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC  
1801  CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT  
1851  CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC  
1901  AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG  
1951  CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC  
2001  AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT  
2051  CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT  
2101  GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA  
2151  GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC  
2201  AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT  
2251  GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA  
2301  TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG  
2351  GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA  
2401  CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA  
2451  AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT  
2501  TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT  
2551  ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA  
2601  GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGACCGCCTG ATGCGGTATT  
2651  TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC  
2701  TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC  
2751  TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC  
2801  GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA  
2851  AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT  
2901  CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA  
2951  AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT  
3001  CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG  
3051  CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG  
3101  TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG  
3151  GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC  
3201  AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA  
3251  TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA  
3301  GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC  
3351  GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT  
3401  GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG  
3451  TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC  
3501  GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA  
3551  ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT  
3601  GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA  
3651  ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC  
3701  CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC  
3751  GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC  
3801  GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC  
3851  CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT  
3901  CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG  
3951  CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC  
4001  AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC  
4051  ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC  
4101  AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG  
4151  ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG  
4201  ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC  
4251  AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC  
4301  TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGAGATCC CGGTGCCTAA  
4351  TGAGTGAGCT AACTTACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA  
4401  GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG  
4451  GGAGAGGCGG TTTGCGTATT GGGCGCCAGG GTGGTTTTTC TTTTCACCAG  
4501  TGAGACGGGC AACAGCTGAT TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT  
4551  GCAGCAAGCG GTCCACGCTG GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG  
4601  ATGGTGGTTA ACGGCGGGAT ATAACATGAG CTGTCTTCGG TATCGTCGTA  
4651  TCCCACTACC GAGATATCCG CACCAACGCG CAGCCCGGAC TCGGTAATGG  
4701  CGCGCATTGC GCCCAGCGCC ATCTGATCGT TGGCAACCAG CATCGCAGTG  
4751  GGAACGATGC CCTCATTCAG CATTTGCATG GTTTGTTGAA AACCGGACAT  
4801  GGCACTCCAG TCGCCTTCCC GTTCCGCTAT CGGCTGAATT TGATTGCGAG  
4851  TGAGATATTT ATGCCAGCCA GCCAGACGCA GACGCGCCGA GACAGAACTT  
4901  AATGGGCCCG CTAACAGCGC GATTTGCTGG TGACCCAATG CGACCAGATG  
4951  CTCCACGCCC AGTCGCGTAC CGTCTTCATG GGAGAAAATA ATACTGTTGA  
5001  TGGGTGTCTG GTCAGAGACA TCAAGAAATA ACGCCGGAAC ATTAGTGCAG  
5051  GCAGCTTCCA CAGCAATGGC ATCCTGGTCA TCCAGCGGAT AGTTAATGAT  
5101  CAGCCCACTG ACGCGTTGCG CGAGAAGATT GTGCACCGCC GCTTTACAGG  
5151  CTTCGACGCC GCTTCGTTCT ACCATCGACA CCACCACGCT GGCACCCAGT  
5201  TGATCGGCGC GAGATTTAAT CGCCGCGACA ATTTGCGACG GCGCGTGCAG  
5251  GGCCAGACTG GAGGTGGCAA CGCCAATCAG CAACGACTGT TTGCCCGCCA  
5301  GTTGTTGTGC CACGCGGTTG GGAATGTAAT TCAGCTCCGC CATCGCCGCT  
5351  TCCACTTTTT CCCGCGTTTT CGCAGAAACG TGGCTGGCCT GGTTCACCAC  
5401  GCGGGAAACG GTCTGATAAG AGACACCGGC ATACTCTGCG ACATCGTATA  
5451  ACGTTACTGG TTTCACATTC ACCACCCTGA ATTGACTGTC TTCCGGGCGC  
5501  TATCATGCCA TACCGCGAAA GGTTTTGCGC CATTCGATGG TGTCCGGGAT  
5551  CTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT  
5601  AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA  
5651  GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC  
5701  CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG  
5751  GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT  
5801  GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA  
5851  AT


Cite error: <ref> tags exist, but no <references/> tag was found