PortEco logo

pBEn-SBPb

From EcoliWiki
Jump to: navigation, search

At a Glance

pBEn-SBPb, Image provided by Stratagene

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 promoter
lac operator
SBP tag[1]
Multiple Cloning Site (MCS)
T7 terminator
lacI repressor ORF

Accession Number




Notes

The SBP tag, a synthetic sequence isolated from a random peptide library, has a high affinity for streptavidin resin (~2 × 10-9 M), and can be effectively eluted from the resin with biotin. The SBP tag has a low positive net charge (+1), making it an ideal purification tag when combined with the SET tags, since its effect on the SET tag negative charge is minimal.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Keefe, AD et al. (2001) One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag. Protein Expr. Purif. 23 440-6 PubMed EcoliWiki page

[1] [2]

Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACGAGAAG  
 101  ACCACCGGCT GGCGGGGCGG CCACGTGGTG GAGGGCCTGG CCGGCGAGCT  
 151  GGAGCAGCTG CGGGCCAGGC TGGAGCACCA CCCTCAGGGC CAGCGGGAGC  
 201  CCTCCGGCGG CTGCAAGCTG GGCCTGCTGG TTCCGCGTGG ATCTCGGATC  
 251  CGAATTCTCT TCCCGGGTCT TGTTCCATGG GTCGACTCGA GCTCAAGCTT  
 301  AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA  
 351  CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG  
 401  AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CCGCAAGAGG  
 451  CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC  
 501  GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT  
 551  GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTAATCG  
 601  AAGATAAGCT GTCAAACATG ACAATTCTTG AAGACGAAAG GGCCTCGTGA  
 651  TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG  
 701  TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT  
 751  TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT  
 801  AAATGCTTCA ATAATATTGA AAAAGGAACA GTATGAGTAT TCAACATTTC  
 851  CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC  
 901  TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG  
 951  CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG  
1001  AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT  
1051  GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG  
1101  GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGACGC GTCACCAGTC  
1151  ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC  
1201  TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA  
1251  TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT  
1301  GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA  
1351  CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA  
1401  AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA  
1451  GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT  
1501  TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT  
1551  CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC  
1601  GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG  
1651  ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG  
1701  ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA  
1751  TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT  
1801  CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA  
1851  TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG  
1901  CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA  
1951  GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC  
2001  CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC  
2051  TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC  
2101  TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT  
2151  AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA  
2201  CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG  
2251  TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT  
2301  ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA  
2351  GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG  
2401  ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA  
2451  AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT  
2501  TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG  
2551  TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG  
2601  AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGACCGCCT GATGCGGTAT  
2651  TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT  
2701  CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG  
2751  CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC  
2801  CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC  
2851  AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA  
2901  TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG  
2951  AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT  
3001  TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG  
3051  GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT  
3101  GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC  
3151  GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA  
3201  CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA  
3251  ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC  
3301  AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC  
3351  CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT  
3401  TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC  
3451  GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC  
3501  CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC  
3551  AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA  
3601  TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG  
3651  AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG  
3701  CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA  
3751  CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC  
3801  CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT  
3851  CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG  
3901  TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG  
3951  GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC  
4001  CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC  
4051  CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC  
4101  CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC  
4151  GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT  
4201  GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA  
4251  CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG  
4301  CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA  
4351  ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC  
4401  AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG  
4451  GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA  
4501  GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT  
4551  TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT  
4601  GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT  
4651  ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG  
4701  GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT  
4751  GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA  
4801  TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA  
4851  GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT  
4901  TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT  
4951  GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG  
5001  ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA  
5051  GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA  
5101  TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG  
5151  GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG  
5201  TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA  
5251  GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC  
5301  AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC  
5351  TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA  
5401  CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT  
5451  AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTGT CTTCCGGGCG  
5501  CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA  
5551  TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG  
5601  TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG  
5651  AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG  
5701  CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC  
5751  GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA  
5801  TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA  
5851  AAT 



Cite error: <ref> tags exist, but no <references/> tag was found