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pBEn-SBPc

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At a Glance

pBEn-SBPc, Image provided by Agilent Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac Operator
SBP tag
Multiple cloning site
lacI repressor

Accession Number




Notes

pBEn-SBPc is a plasmid with a multiple cloning site region. Contained in this plasmid are features such as T7 promoter, extended RBS, SBP tag, multiple cloning site, T7 terminator, ampicillin resistance, pBR322 origin, and lasI repressor open reading frame.

History

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Sources

Agilent-Genomics

References

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Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACGAGAAG  
 101  ACCACCGGCT GGCGGGGCGG CCACGTGGTG GAGGGCCTGG CCGGCGAGCT  
 151  GGAGCAGCTG CGGGCCAGGC TGGAGCACCA CCCTCAGGGC CAGCGGGAGC  
 201  CCTCCGGCGG CTGCAAGCTG GGCCTGCTGG TTCCGCGTGG ATCTGCGGAT  
 251  CCGAATTCTC TTCCCGGGTC TTGTTCCATG GGTCGACTCG AGCTCAAGCT  
 301  TAGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC  
 351  ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT  
 401  GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCCGCAAGAG  
 451  GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA  
 501  CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC  
 551  TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTAATC  
 601  GAAGATAAGC TGTCAAACAT GACAATTCTT GAAGACGAAA GGGCCTCGTG  
 651  ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC  
 701  GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT  
 751  TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA  
 801  TAAATGCTTC AATAATATTG AAAAAGGAAC AGTATGAGTA TTCAACATTT  
 851  CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG  
 901  CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT  
 951  GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA  
1001  GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC  
1051  TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC  
1101  GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGACG CGTCACCAGT  
1151  CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG  
1201  CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG  
1251  ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA  
1301  TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA  
1351  ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC   
1401  AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT  
1451  AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC  
1501  TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG  
1551  TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT  
1601  CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA  
1651  GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA  
1701  GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA  
1751  ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA  
1801  TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG  
1851  ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT  
1901  GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG  
1951  AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA  
2001  CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA  
2051  CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG  
2101  CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA  
2151  TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC  
2201  ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC  
2251  GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG  
2301  TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC  
2351  AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT  
2401  GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG  
2451  AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC  
2501  TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC  
2551  GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC  
2601  GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGACCGCC TGATGCGGTA  
2651  TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC  
2701  TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC  
2751  GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC  
2801  CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA  
2851  CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC  
2901  ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT  
2951  GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT  
3001  TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG  
3051  GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC  
3101  TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA  
3151  CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA  
3201  ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC  
3251  AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG  
3301  CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT  
3351  CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG  
3401  TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG  
3451  CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG  
3501  CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC  
3551  CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG  
3601  ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA  
3651  GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC  
3701  GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA  
3751  ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC  
3801  CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG  
3851  TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT  
3901  GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG  
3951  GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT  
4001  CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG  
4051  CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA  
4101  CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG  
4151  CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA  
4201  TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG  
4251  ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA  
4301  GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGAGAT CCCGGTGCCT  
4351  AATGAGTGAG CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC  
4401  CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC  
4451  GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA GGGTGGTTTT TCTTTTCACC  
4501  AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC CCTGAGAGAG  
4551  TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT  
4601  TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG  
4651  TATCCCACTA CCGAGATATC CGCACCAACG CGCAGCCCGG ACTCGGTAAT  
4701  GGCGCGCATT GCGCCCAGCG CCATCTGATC GTTGGCAACC AGCATCGCAG  
4751  TGGGAACGAT GCCCTCATTC AGCATTTGCA TGGTTTGTTG AAAACCGGAC  
4801  ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA TTTGATTGCG  
4851  AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC  
4901  TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA  
4951  TGCTCCACGC CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT  
5001  GATGGGTGTC TGGTCAGAGA CATCAAGAAA TAACGCCGGA ACATTAGTGC  
5051  AGGCAGCTTC CACAGCAATG GCATCCTGGT CATCCAGCGG ATAGTTAATG  
5101  ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG CCGCTTTACA  
5151  GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA  
5201  GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGGCGCGTGC  
5251  AGGGCCAGAC TGGAGGTGGC AACGCCAATC AGCAACGACT GTTTGCCCGC  
5301  CAGTTGTTGT GCCACGCGGT TGGGAATGTA ATTCAGCTCC GCCATCGCCG  
5351  CTTCCACTTT TTCCCGCGTT TTCGCAGAAA CGTGGCTGGC CTGGTTCACC  
5401  ACGCGGGAAA CGGTCTGATA AGAGACACCG GCATACTCTG CGACATCGTA  
5451  TAACGTTACT GGTTTCACAT TCACCACCCT GAATTGACTG TCTTCCGGGC  
5501  GCTATCATGC CATACCGCGA AAGGTTTTGC GCCATTCGAT GGTGTCCGGG  
5551  ATCTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA  
5601  GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG  
5651  GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC  
5701  GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT  
5751  CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG  
5801  ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG  
5851  AAATCatergory:PBR322 Origin