PortEco logo

pBEn-SET1a

From EcoliWiki
Jump to: navigation, search

At a Glance

pBEn-SET1a, Image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac Operator
extended RBS
MCS
lacI repressor ORF

Accession Number




Notes

Use when want to use the VariFlex tags at the N-terminus of your protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

[back to top]


See Help:References for how to manage references in EcoliWiki.


Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCACGCACC CGCGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG  
 201  CTGCCACCGC TGAGCAACTG CTGGTTCCGC GTGGATCTGG ATCCGAATTC  
 251  TCTTCCCGGG TCTTGTTCCA TGGGTCGACT CGAGCTCAAG CTTAGATCCG  
 301  GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA  
 351  GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT  
 401  TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCCGCAAG AGGCCCGGCA  
 451  GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG  
 501  AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG  
 551  TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTAA TCGAAGATAA  
 601  GCTGTCAAAC ATGACAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT  
 651  ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG  
 701  GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA  
 751  ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT  
 801  TCAATAATAT TGAAAAAGGA ACAGTATGAG TATTCAACAT TTCCGTGTCG  
 851  CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA  
 901  GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT  
 951  GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC  
1001  GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT  
1051  GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG  
1101  CATACACTAT TCTCAGAATG ACTTGGTTGA CGCGTCACCA GTCACAGAAA  
1151  AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA  
1201  ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG  
1251  ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC  
1301  GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG  
1351  CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT  
1401  AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA  
1451  TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT  
1501  GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG  
1551  TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA  
1601  TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC  
1651  GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT  
1701  TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA  
1751  GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA  
1801  CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG  
1851  ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA  
1901  AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA  
1951  ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC  
2001  TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG  
2051  CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC  
2101  AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC  
2151  GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA  
2201  GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA  
2251  TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT  
2301  AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA  
2351  ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG  
2401  CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC  
2451  CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC  
2501  ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC  
2551  GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG  
2601  CGAGTCAGTG AGCGAGGAAG CGGAAGACCG CCTGATGCGG TATTTTCTCC  
2651  TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA  
2701  CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC  
2751  TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC  
2801  GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT  
2851  GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA  
2901  AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT  
2951  TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG  
3001  AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT  
3051  TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG  
3101  GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC  
3151  TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG  
3201  CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG  
3251  CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC  
3301  TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT  
3351  CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG  
3401  GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG  
3451  TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC  
3501  TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG  
3551  CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT  
3601  GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT  
3651  TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT  
3701  CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG  
3751  AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA  
3801  TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA  
3851  TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA  
3901  TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC  
3951  GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC  
4001  GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG  
4051  GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC  
4101  GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC  
4151  CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG  
4201  AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT  
4251  AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG  
4301  GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG  
4351  AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG  
4401  AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG  
4451  GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC  
4501  GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA  
4551  AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG  
4601  GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC  
4651  TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA  
4701  TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG  
4751  ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT  
4801  CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT  
4851  ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG  
4901  CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC  
4951  GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG  
5001  TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT  
5051  TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC  
5101  ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA  
5151  CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG  
5201  GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG  
5251  ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT  
5301  GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT  
5351  TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA  
5401  AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA  
5451  CTGGTTTCAC ATTCACCACC CTGAATTGAC TGTCTTCCGG GCGCTATCAT  
5501  GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC  
5551  GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG  
5601  AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC  
5651  GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC  
5701  AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG  
5751  TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC  
5801  CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT