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pBEn-SET1b

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At a Glance

pBEn-SET1b, Image provided by Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Promoter
lac Operator
extended RBS
SET1 tag
MCS
lacI repressor ORF

Accession Number




Notes

Use pBEn vectors when want to use VariFlex tags at N-terminus of protein of interest.

History

You can help EcoliWiki by writing about the history of this plasmid.

Sources

Agilent-Genomics

References

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Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCACGCACC CGCGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG  
 201  CTGCCACCGC TGAGCAACTG CTGGTTCCGC GTGGATCTCG GATCCGAATT  
 251  CTCTTCCCGG GTCTTGTTCC ATGGGTCGAC TCGAGCTCAA GCTTAGATCC  
 301  GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG  
 351  AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT  
 401  TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCCGCAA GAGGCCCGGC  
 451  AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC  
 501  GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC  
 551  GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTA ATCGAAGATA  
 601  AGCTGTCAAA CATGACAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC  
 651  TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT  
 701  GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA  
 751  AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC  
 801  TTCAATAATA TTGAAAAAGG AACAGTATGA GTATTCAACA TTTCCGTGTC  
 851  GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC  
 901  AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG  
 951  TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT  
1001  CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG  
1051  TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC  
1101  GCATACACTA TTCTCAGAAT GACTTGGTTG ACGCGTCACC AGTCACAGAA  
1151  AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT  
1201  AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG  
1251  GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT  
1301  CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA  
1351  GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT  
1401  TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG  
1451  ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC  
1501  TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG  
1551  GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT  
1601  ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT  
1651  CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG  
1701  TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA  
1751  AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA  
1801  ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG  
1851  GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA  
1901  AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC  
1951  AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA  
2001  CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA  
2051  GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC  
2101  CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC  
2151  CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC  
2201  AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT  
2251  ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG  
2301  TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA  
2351  AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA  
2401  GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG  
2451  CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT  
2501  CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC  
2551  CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA  
2601  GCGAGTCAGT GAGCGAGGAA GCGGAAGACC GCCTGATGCG GTATTTTCTC  
2651  CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT  
2701  ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG  
2751  CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA  
2801  CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG  
2851  TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG  
2901  AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA  
2951  TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA  
3001  GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT  
3051  TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT  
3101  GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA  
3151  CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG  
3201  GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA  
3251  GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC  
3301  CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT  
3351  TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA  
3401  GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG  
3451  GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC  
3501  CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT  
3551  GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA  
3601  TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA  
3651  TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT  
3701  TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG  
3751  GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC  
3801  ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG  
3851  ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG  
3901  ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC  
3951  CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT  
4001  CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC  
4051  GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA  
4101  CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG  
4151  CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA  
4201  GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA  
4251  TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT  
4301  GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT  
4351  GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG  
4401  GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA  
4451  GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA  
4501  CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC  
4551  AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT  
4601  GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA  
4651  CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC  
4701  ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC  
4751  GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC  
4801  TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA  
4851  TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG  
4901  GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA  
4951  CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT  
5001  GTCTGGTCAG AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC  
5051  TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA ATGATCAGCC  
5101  CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG  
5151  ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC  
5201  GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA  
5251  GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT  
5301  TGTGCCACGC GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC  
5351  TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC ACCACGCGGG  
5401  AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT  
5451  ACTGGTTTCA CATTCACCAC CCTGAATTGA CTGTCTTCCG GGCGCTATCA  
5501  TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA  
5551  CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT  
5601  GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG  
5651  CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA  
5701  CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT  
5751  GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG  
5801  CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT