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pBEn-SET3c

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At a Glance

pBEn-SET3c From Agilent-Genomics

Type

Expression

Origin of Replication

pBR322

Copy #
Markers

Ampicillin

Link to Sequence

Agilent-Genomics

Features

T7 Operator
lac Operator
extended RBS
MCS
lacI repressor ORF

Accession Number




Notes

Use pBEn vectors when want to use VariFlex tags at N-terminus of protein of interest.

History

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Sources

Agilent-Genomics

References

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Sequence

   1  TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA  
  51  GAAATAATTT TGTTTAACTT TAAGAAGGAG ATATACATAT GGACCCCGAA  
 101  GAGGCGAGTG TTACTTCAAC AGAAGAAACC TTAACGCCAG CACAGGAGGC  
 151  CGCAGAGACC GAAGCTGCTA ACAAAGCCGA AGAGGAAGCT GAGTTGGAGG  
 201  CTGAAACCGC TGAGCAACTG CTGGTTCCGC GTGGATCTGC GGATCCGAAT  
 251  TCTCTTCCCG GGTCTTGTTC CATGGGTCGA CTCGAGCTCA AGCTTAGATC  
 301  CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT  
 351  GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG  
 401  TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCCGCA AGAGGCCCGG   
 451  CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC  
 501  CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG  
 551  CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT AATCGAAGAT  
 601  AAGCTGTCAA ACATGACAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC  
 651  CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG  
 701  TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT  
 751  AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG  
 801  CTTCAATAAT ATTGAAAAAG GAACAGTATG AGTATTCAAC ATTTCCGTGT  
 851  CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC  
 901  CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA  
 951  GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT  
1001  TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT  
1051  GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC  
1101  CGCATACACT ATTCTCAGAA TGACTTGGTT GACGCGTCAC CAGTCACAGA  
1151  AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA  
1201  TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA  
1251  GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC  
1301  TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG  
1351  AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA  
1401  TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG  
1451  GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG  
1501  CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC  
1551  GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT  
1601  TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA  
1651  TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA  
1701  GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA  
1751  AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT  
1801  AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA  
1851  GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC  
1901  AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC  
1951  CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT  
2001  ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT  
2051  AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG  
2101  CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA  
2151  CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC  
2201  CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC  
2251  TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG  
2301  GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG  
2351  AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG  
2401  AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC  
2451  GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC  
2501  TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA  
2551  CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC  
2601  AGCGAGTCAG TGAGCGAGGA AGCGGAAGAC CGCCTGATGC GGTATTTTCT  
2651  CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG  
2701  TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC  
2751  GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG  
2801  ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT  
2851  GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC  
2901  GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG  
2951  ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC  
3001  AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT  
3051  TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA  
3101  TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT  
3151  ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT  
3201  GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC  
3251  AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT  
3301  CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT  
3351  TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC  
3401  AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC  
3451  GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT  
3501  CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC  
3551  TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT  
3601  ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG  
3651  ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC  
3701  TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG  
3751  GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC  
3801  CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT  
3851  GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT  
3901  GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC  
3951  CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC  
4001  TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC  
4051  CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG  
4101  ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG  
4151  GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC  
4201  AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC  
4251  ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC  
4301  TGGGTTGAAG GCTCTCAAGG GCATCGGTCG AGATCCCGGT GCCTAATGAG  
4351  TGAGCTAACT TACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG  
4401  GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG  
4451  AGGCGGTTTG CGTATTGGGC GCCAGGGTGG TTTTTCTTTT CACCAGTGAG  
4501  ACGGGCAACA GCTGATTGCC CTTCACCGCC TGGCCCTGAG AGAGTTGCAG  
4551  CAAGCGGTCC ACGCTGGTTT GCCCCAGCAG GCGAAAATCC TGTTTGATGG  
4601  TGGTTAACGG CGGGATATAA CATGAGCTGT CTTCGGTATC GTCGTATCCC  
4651  ACTACCGAGA TATCCGCACC AACGCGCAGC CCGGACTCGG TAATGGCGCG  
4701  CATTGCGCCC AGCGCCATCT GATCGTTGGC AACCAGCATC GCAGTGGGAA  
4751  CGATGCCCTC ATTCAGCATT TGCATGGTTT GTTGAAAACC GGACATGGCA  
4801  CTCCAGTCGC CTTCCCGTTC CGCTATCGGC TGAATTTGAT TGCGAGTGAG  
4851  ATATTTATGC CAGCCAGCCA GACGCAGACG CGCCGAGACA GAACTTAATG  
4901  GGCCCGCTAA CAGCGCGATT TGCTGGTGAC CCAATGCGAC CAGATGCTCC  
4951  ACGCCCAGTC GCGTACCGTC TTCATGGGAG AAAATAATAC TGTTGATGGG  
5001  TGTCTGGTCA GAGACATCAA GAAATAACGC CGGAACATTA GTGCAGGCAG  
5051  CTTCCACAGC AATGGCATCC TGGTCATCCA GCGGATAGTT AATGATCAGC  
5101  CCACTGACGC GTTGCGCGAG AAGATTGTGC ACCGCCGCTT TACAGGCTTC  
5151  GACGCCGCTT CGTTCTACCA TCGACACCAC CACGCTGGCA CCCAGTTGAT  
5201  CGGCGCGAGA TTTAATCGCC GCGACAATTT GCGACGGCGC GTGCAGGGCC  
5251  AGACTGGAGG TGGCAACGCC AATCAGCAAC GACTGTTTGC CCGCCAGTTG  
5301  TTGTGCCACG CGGTTGGGAA TGTAATTCAG CTCCGCCATC GCCGCTTCCA  
5351  CTTTTTCCCG CGTTTTCGCA GAAACGTGGC TGGCCTGGTT CACCACGCGG  
5401  GAAACGGTCT GATAAGAGAC ACCGGCATAC TCTGCGACAT CGTATAACGT  
5451  TACTGGTTTC ACATTCACCA CCCTGAATTG ACTGTCTTCC GGGCGCTATC  
5501  ATGCCATACC GCGAAAGGTT TTGCGCCATT CGATGGTGTC CGGGATCTCG  
5551  ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT  
5601  TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG  
5651  GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA  
5701  ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA  
5751  TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG  
5801  GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT