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pBiEx-1

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At a Glance

pBiEx-1, Image made with PlasMapper

Type

Expression

Origin of Replication

pUC

Copy #

High

Markers

Ampicillin

Link to Sequence

Novagen

Features

hr5 enhancer
IE1 promoter
T7 promoter
His•Tag
S•Tag
Multiple cloning sites
HSV•Tag
lacI coding sequence

Accession Number




Notes

The vectors feature the same hr5 enhancer and the ie1 (immediate early) promoter to direct expression in insect cells as the pIEx™ Vectors, and also allow for expression in E. coli by the tightly controlled T7lac promoter.

History

The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.

Sources

Novagen

References

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Sequence

       1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat 
      61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca 
     121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg 
     181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata 
     241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac 
     301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg 
     361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa 
     421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac 
     481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg 
     541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg 
     601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag 
     661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg 
     721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga 
     781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta 
     841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg 
     901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca 
     961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt 
    1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg 
    1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct 
    1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcacaccat caccaccatc 
    1201 actcttctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgccac 
    1261 cgccttctgg tctggtcccc cggggcagcg caggttctgg tacgattgat gacgacgaca 
    1321 agatctctgg cccgggctcg gatcccaatt gggagctcgt gtacacggcg cgcctgcagg 
    1381 tcgacggtac cggttcgaag cttgcggccg cacagctgta tacacgtgca agccagccag 
    1441 aactcgcccc ggaagacccc gaggatctcg agcactaagt gattaacctc agccgaaagg 
    1501 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta 
    1561 aacgggtctt gaggggtttt ttgctgaaag gaggaactat cctcaggtta tacatatatt 
    1621 ttgaatttaa ttaattatac atatatttta tattattttt gtcttttatt atcgaggggc 
    1681 cgttgttggt gtggggtttt gcatagaaat aacaatggga gttggcgacg ttgctgcgcc 
    1741 aacaccacct cccttccctc ctttcatcat gtatctgtag ataaaataaa atattaaacc 
    1801 taaaaacaag accgcgccta tcaacaaaat gataggcatt aacttgccgc tgacgctgtc 
    1861 actaacgttg gacgatttgc cgactaaacc ttcatcgccc agtaaccaat ctagacgtca 
    1921 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca 
    1981 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac 
    2041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
    2101 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg 
    2161 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg 
    2221 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat 
    2281 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg 
    2341 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca 
    2401 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc 
    2461 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag 
    2521 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc 
    2581 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa 
    2641 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg 
    2701 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca 
    2761 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt 
    2821 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg 
    2881 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac 
    2941 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc 
    3001 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa 
    3061 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca 
    3121 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg 
    3181 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa 
    3241 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatggac 
    3301 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
    3361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
    3421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
    3481 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
    3541 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
    3601 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
    3661 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
    3721 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
    3781 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
    3841 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
    3901 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
    3961 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
    4021 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
    4081 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
    4141 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
    4201 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
    4261 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
    4321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
    4381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
    4441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
    4501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
    4561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 
    4621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
    4681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
    4741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
    4801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
    4861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
    4921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
    4981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
    5041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
    5101 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 
    5161 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 
    5221 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
    5281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
    5341 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
    5401 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
    5461 ttacgaattc ccggg