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pBiEx-2

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At a Glance

pBiEx-2, Image made with PlasMapper

Type

Expression

Origin of Replication

pUC

Copy #

High

Markers

Ampicillin

Link to Sequence

Novagen

Features

hr5 enhancer
IE1 promoter
T7 promoter
GST•Tag
His•Tag
S•Tag
Multiple cloning sites
HSV•Tag
lacI

Accession Number




Notes

The vectors feature the same hr5 enhancer and the ie1 (immediate early) promoter to direct expression in insect cells as the pIEx™ Vectors, and also allow for expression in E. coli by the tightly controlled T7lac promoter.

History

The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.

Sources

Novagen

References

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Sequence

       1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat 
      61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca 
     121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg 
     181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata 
     241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac 
     301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg 
     361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa 
     421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac 
     481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg 
     541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg 
     601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag 
     661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg 
     721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga 
     781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta 
     841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg 
     901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca 
     961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt 
    1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg 
    1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct 
    1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcccct atactaggtt 
    1201 attggaaaat taagggcctt gtgcaaccca ctcgacttct tttggaatat cttgaagaaa 
    1261 aatatgaaga gcatttgtat gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg 
    1321 aattgggttt ggagtttccc aatcttcctt attatattga tggtgatgtt aaattaacac 
    1381 agtctatggc catcatacgt tatatagctg acaagcacaa catgttgggt ggttgtccaa 
    1441 aagagcgtgc agagatttca atgcttgaag gagcggtttt ggatattaga tacggtgttt 
    1501 cgagaattgc atatagtaaa gactttgaaa ctctcaaagt tgattttctt agcaagctac 
    1561 ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa aacatattta aatggtgatc 
    1621 atgtaaccca tcctgacttc atgttgtatg acgctcttga tgttgtttta tacatggacc 
    1681 caatgtgcct ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt gaagctatcc 
    1741 cacaaattga taagtacttg aaatccagca agtatatagc atggcctttg cagggctggc 
    1801 aagccacgtt tggtggtggc gaccatcctc caaaatcgga tggttcaact agtggttctg 
    1861 gttccatggc acaccatcac caccatcact cttctggtaa agaaaccgct gctgcgaaat 
    1921 ttgaacgcca gcacatggac tcgccaccgc cttctggtct ggtcccccgg ggcagcgcag 
    1981 gttctggtac gattgatgac gacgacaaga tctctggccc gggctcggat cccaattggg 
    2041 agctcgtgta cacggcgcgc ctgcaggtcg acggtaccgg ttcgaagctt gcggccgcac 
    2101 agctgtatac acgtgcaagc cagccagaac tcgccccgga agaccccgag gatctcgagc 
    2161 actaagtgat taacctcagc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 
    2221 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag 
    2281 gaactatcct caggttatac atatattttg aatttaatta attatacata tattttatat 
    2341 tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca tagaaataac 
    2401 aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt tcatcatgta 
    2461 tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca acaaaatgat 
    2521 aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga ctaaaccttc 
    2581 atcgcccagt aaccaatcta gacgtcacga tagtcatgcc ccgcgcccac cggaaggagc 
    2641 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct 
    2701 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
    2761 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg 
    2821 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc 
    2881 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg 
    2941 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc 
    3001 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc 
    3061 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg 
    3121 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt 
    3181 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt 
    3241 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc 
    3301 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca 
    3361 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca 
    3421 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc 
    3481 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt 
    3541 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg 
    3601 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg 
    3661 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg 
    3721 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg 
    3781 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc 
    3841 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc 
    3901 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac 
    3961 cgccgccgca aggaatggtg catggacgtc aggtggcact tttcggggaa atgtgcgcgg 
    4021 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 
    4081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 
    4141 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 
    4201 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 
    4261 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 
    4321 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 
    4381 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 
    4441 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 
    4501 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 
    4561 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 
    4621 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac 
    4681 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 
    4741 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 
    4801 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 
    4861 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 
    4921 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 
    4981 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 
    5041 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 
    5101 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 
    5161 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 
    5221 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 
    5281 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 
    5341 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 
    5401 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 
    5461 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 
    5521 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 
    5581 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 
    5641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 
    5701 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 
    5761 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 
    5821 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 
    5881 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc 
    5941 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 
    6001 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 
    6061 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 
    6121 tcacacagga aacagctatg accatgatta cgaattcccg gg