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pBiEx-3

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At a Glance

pBiEx-3, Image made with PlasMapper

Type

Expression

Origin of Replication

pUC

Copy #

High

Markers

Ampicillin

Link to Sequence

Novagen

Features

hr5 enhancer
IE1 promoter
T7 promoter
Multiple cloning sites
S•Tag
His•Tag
lacI coding sequence

Accession Number




Notes

In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and IE1 immediate early promoter.

History

The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells.

Sources

Novagen

References

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Sequence

       1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat 
      61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca 
     121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg 
     181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata 
     241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac 
     301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg 
     361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa 
     421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac 
     481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg 
     541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg 
     601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag 
     661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg 
     721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga 
     781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta 
     841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg 
     901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca 
     961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt 
    1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg 
    1081 accgcggaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct 
    1141 agaaataatt ttgtttaact ttaagaagga gatataccat ggcatcgtta acacgtcaag 
    1201 agctcgcgga tcccaattgg cagatctcgg cgcgcctgca ggtcgacggt accggttcga 
    1261 agcttgcggc cgcatcttct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca 
    1321 tggactcgcc accgccttct ggcctcgagc accaccatca ccatcaccat cactaagtga 
    1381 ttaacctcag ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat 
    1441 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatcc 
    1501 tcaggttata catatatttt gaatttaatt aattatacat atattttata ttatttttgt 
    1561 cttttattat cgaggggccg ttgttggtgt ggggttttgc atagaaataa caatgggagt 
    1621 tggcgacgtt gctgcgccaa caccacctcc cttccctcct ttcatcatgt atctgtagat 
    1681 aaaataaaat attaaaccta aaaacaagac cgcgcctatc aacaaaatga taggcattaa 
    1741 cttgccgctg acgctgtcac taacgttgga cgatttgccg actaaacctt catcgcccag 
    1801 taaccaatct agacgtcacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt 
    1861 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat 
    1921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
    1981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt 
    2041 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt 
    2101 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt 
    2161 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc 
    2221 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg 
    2281 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga 
    2341 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga 
    2401 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc 
    2461 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta 
    2521 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat 
    2581 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga 
    2641 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag 
    2701 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg 
    2761 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca 
    2821 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa 
    2881 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc 
    2941 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat 
    3001 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc 
    3061 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg 
    3121 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc 
    3181 aaggaatggt gcatggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 
    3241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 
    3301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 
    3361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 
    3421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 
    3481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 
    3541 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 
    3601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 
    3661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 
    3721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 
    3781 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 
    3841 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 
    3901 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 
    3961 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 
    4021 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 
    4081 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 
    4141 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 
    4201 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 
    4261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 
    4321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 
    4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 
    4441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 
    4501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 
    4561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 
    4621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 
    4681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 
    4741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 
    4801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 
    4861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 
    4921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 
    4981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 
    5041 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 
    5101 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 
    5161 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 
    5221 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 
    5281 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 
    5341 aaacagctat gaccatgatt acgaattccc ggg